FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_29.3410000000aeb0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_29.3410000000aeb0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3753
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC3729.91207034372502No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT481.2789768185451638No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.6128430588862244No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA200.5329070077271516No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG180.4796163069544364No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.4796163069544364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.4263256061817213No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA150.3996802557953637No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.37303490540900613No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.3463895550226485No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA100.2664535038635758No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA90.2398081534772182No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.2398081534772182No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.18651745270450307No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA70.18651745270450307No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA70.18651745270450307No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG60.15987210231814547No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC50.1332267519317879RNA PCR Primer, Index 23 (95% over 21bp)
GCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT50.1332267519317879No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA50.1332267519317879No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA50.1332267519317879No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA40.10658140154543032No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10658140154543032No Hit
GAGTACGGGGAATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACG40.10658140154543032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT40.10658140154543032No Hit
GGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAGATAC40.10658140154543032No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT40.10658140154543032No Hit
GTATCAACGCAGAGTACGGGCAGCTGTCTCTTATACACATCTCCGAGCCCA40.10658140154543032No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.10658140154543032No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC358.385177E-844.98666445
GGATGTG456.044156E-734.98962830
AGCCCAC456.044156E-734.98962817
GTATGCC456.044156E-734.98962842
ATCTCGT456.044156E-734.98962837
GCCCACG456.044156E-734.98962818
GACAGGA456.044156E-734.98962826
AGGATGT456.044156E-734.98962829
CCACGAG456.044156E-734.98962820
TGATCTC456.044156E-734.98962835
TATGCCG456.044156E-734.98962843
GATGTGA456.044156E-734.98962831
GAGCCCA456.044156E-734.98962816
CACGAGA456.044156E-734.98962821
CCCACGA456.044156E-734.98962819
ATGTGAT456.044156E-734.98962832
TCTCGTA456.044156E-734.98962838
ACGAGAC456.044156E-734.98962822
GATCTCG456.044156E-734.98962836
ATGCCGT456.044156E-734.98962844
Produced by FastQC (version 0.11.2)