FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_28.3410000000aea4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_28.3410000000aea4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC60.336322869955157RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC50.2802690582959641No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC50.2802690582959641No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC40.2242152466367713No Hit
CTCCAACAGTTGTGGAGGAGGAGAAGAAAGCTGAAGAAGTGACTGAAACTC20.11210762331838565No Hit
GTCAGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCGCCGCGTGC20.11210762331838565No Hit
GGGTTCGTCCTTCTTCTCCTCCTTAGCAGGGGCAGCAGCAGCAGCTCCTCC20.11210762331838565No Hit
GTTGCGACTGGAGGGGGAGTTGCGGGAGGAGGAGTGGCGGTGGGAGTAGGA20.11210762331838565No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA20.11210762331838565No Hit
GCTCATTCTCTACGGACTCTACAAGCAAGCCAAGTTTGGGCCTGTGGACAC20.11210762331838565No Hit
CCATTATGGAGGAAGAACAAATAGATCGGATCCATCACGTCAATGAATGAA20.11210762331838565No Hit
GATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAGC20.11210762331838565No Hit
CTTCAGTTTTGGGAAGTTTGTGATTATGGACGACCCTGTGACAATGGACCA20.11210762331838565No Hit
ATCTTATCCTGATTGTACTTACGAGCAAGCATCATCAAGGAAGGCTCAATA20.11210762331838565No Hit
GGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGCG20.11210762331838565No Hit
GTTTCAACTTGAGCTTCCTCTGTTTCTTCGATTGGTTTCTCTGTCTCTTCC20.11210762331838565No Hit
GTCATATAGAGCTTCTTCCTTCCTCTTTACTCCTTGGCCTTCTCGACGGCG20.11210762331838565No Hit
CTTCGAAAGGGGATCCGGTTAAAATTCCGGAACCGGGACGTGGCGGTTGAC20.11210762331838565No Hit
CTCTAATGGTAACATTCGTAGAGCTTAATATCAAGCTTGACGCGGAAATAC20.11210762331838565No Hit
ATCTAACGTCGGAGGTAGACGGATCTCACCGGCTCCTTCACCTAAGAAGAT20.11210762331838565No Hit
CTGCAACATGAATGTATTGTAAAACGTTTTTGCTCCAAAATACTACAACTG20.11210762331838565No Hit
CCTTCACTTTGTGGAGACACCAGCGCTTGCTCCACCAGAGGTTCACATTGA20.11210762331838565No Hit
AAAGAGCACAAGCCAGATTCCTTAAGTTTTAAGACAACACTATAAGGACCC20.11210762331838565No Hit
ACATATATTCAAATGGCCACCATCGAGGTTGAACAAGTGACTCCAGTAGCA20.11210762331838565No Hit
GCCGTGATCGTGGTCTGCAGCACGCGCCTAACGGCGTGCCTCGGCATCAGC20.11210762331838565No Hit
GCTTACGACCCCATCCCCAACTTCGTCTTTTTTGAAGAGGGTACAAAACCT20.11210762331838565No Hit
CTCAAGAACCACCTAGAGATAACCCAATGGAGGCAATGAAGATGAGACTCT20.11210762331838565No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)