FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_27.3410000000ae97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_27.3410000000ae97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1942076
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC20180.1039094247598961RNA PCR Primer, Index 23 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGT552.752131E-936.8166920
TGCCGTC2600.036.34467345
CGAGCCC3000.031.49872415
CCGAGCC3050.030.98234614
GCCCACG3100.030.48263418
TATGCCG3300.028.63520243
ATGCCGT3550.027.8861944
CCCACGA3550.027.25241519
CGTATGC3650.026.50577441
CGAGACG3500.026.35607123
AGCCCAC3600.026.24893417
GTATGCC3650.025.8893642
ACGGACG801.05499566E-725.31147219
TCCGAGC3850.025.12884913
GAGCCCA3850.024.54445616
CCACGAG3900.024.22978420
TGCTGGC1152.6557245E-1023.47730612
GTCCCCT500.002241741922.5402891
TCGTATG4850.020.87543940
ACGTCCC550.003938426320.4537147
Produced by FastQC (version 0.11.2)