FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_26.3410000000ae8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_26.3410000000ae8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241771
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC3220.1331838806143003RNA PCR Primer, Index 45 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC356.2284325E-638.57089641
TGCCGTC301.1373496E-437.4994845
GTATGCC401.5556407E-533.74953542
AGCCCAC352.814064E-432.14241417
TATGCCG352.814064E-432.14241443
CGTTCAT352.814064E-432.14241428
CGAGCCC352.814064E-432.14241415
GCCCACG406.15245E-428.12461318
CTCGTAT406.15245E-428.12461339
CCCACGA406.15245E-428.12461319
CCGAGCC406.15245E-428.12461314
CGAGACG406.15245E-428.12461323
TCGTATG507.1361035E-526.99962840
GATCGTA450.001223852424.99965526
TCCGAGC450.001223852424.99965513
GAGCCCA450.001223852424.99965516
CCACGAG450.001223852424.99965520
ACGTTCA500.0022596622.49969127
GACGTTC500.0022596622.49969126
GAGACGT500.0022596622.49969124