Basic Statistics
Measure | Value |
---|---|
Filename | H23V3BCXX l02n01 kb2_4_26.3410000000ae8a.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 241771 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 37 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC | 322 | 0.1331838806143003 | RNA PCR Primer, Index 45 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 35 | 6.2284325E-6 | 38.570896 | 41 |
TGCCGTC | 30 | 1.1373496E-4 | 37.49948 | 45 |
GTATGCC | 40 | 1.5556407E-5 | 33.749535 | 42 |
AGCCCAC | 35 | 2.814064E-4 | 32.142414 | 17 |
TATGCCG | 35 | 2.814064E-4 | 32.142414 | 43 |
CGTTCAT | 35 | 2.814064E-4 | 32.142414 | 28 |
CGAGCCC | 35 | 2.814064E-4 | 32.142414 | 15 |
GCCCACG | 40 | 6.15245E-4 | 28.124613 | 18 |
CTCGTAT | 40 | 6.15245E-4 | 28.124613 | 39 |
CCCACGA | 40 | 6.15245E-4 | 28.124613 | 19 |
CCGAGCC | 40 | 6.15245E-4 | 28.124613 | 14 |
CGAGACG | 40 | 6.15245E-4 | 28.124613 | 23 |
TCGTATG | 50 | 7.1361035E-5 | 26.999628 | 40 |
GATCGTA | 45 | 0.0012238524 | 24.999655 | 26 |
TCCGAGC | 45 | 0.0012238524 | 24.999655 | 13 |
GAGCCCA | 45 | 0.0012238524 | 24.999655 | 16 |
CCACGAG | 45 | 0.0012238524 | 24.999655 | 20 |
ACGTTCA | 50 | 0.00225966 | 22.499691 | 27 |
GACGTTC | 50 | 0.00225966 | 22.499691 | 26 |
GAGACGT | 50 | 0.00225966 | 22.499691 | 24 |