FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_24.3410000000ae60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_24.3410000000ae60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152525
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA12740.8352729060809703No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9150.599901655466317No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8750.5736764464841829No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA7420.4864776266185871No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA5550.3638747746271103No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC5380.35272906080970334No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5380.35272906080970334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5140.3369939354204229No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.33043763317488933No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3990.2615964595967874No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3500.22947057859367317No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2940.19275528601868547No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2670.17505326995574497No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2610.17111948860842482No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2510.16456318636289133No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2160.141616128503524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2040.1337485658088838No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1780.11670217997049664No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1540.1009670545812162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC501.0695658E-940.49793242
TTGTCGG301.1356259E-437.4980922
TCCGAGC552.7175702E-936.81630313
CCCACGA552.7175702E-936.81630319
ATGCCGT552.7175702E-936.81630344
GCAACTT250.002101921235.9981621
CGGCATC702.0008883E-1135.3553433
ACGAGAC653.619789E-1034.6136222
TCTCGTA606.357368E-933.7482838
CGAGACT708.021743E-1032.14121623
CGTATGC651.3873432E-831.15225841
CTCGTAT651.3873432E-831.15225839
CACGAGA651.3873432E-831.15225821
TCGTATG651.3873432E-831.15225840
TGCCGTC651.3873432E-831.15225845
TATGCCG702.8530849E-828.92709543
GACGGCA702.8530849E-828.92709531
CTCGACG702.8530849E-828.92709528
CCACGAG702.8530849E-828.92709520
TCGACGG702.8530849E-828.92709529
Produced by FastQC (version 0.11.2)