FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_23.3410000000ae54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_23.3410000000ae54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC32.054794520547945No Hit
ATGTAATTCCAGAATTCCGAAAACACAATCCACACTGAAGATATACCCTGT10.684931506849315No Hit
CGCTATGACTATGTTGGAACTAAGATATTCTTGAAGCACAGGTATTTCCCT10.684931506849315No Hit
TTTCTACTTTGAGAACTACTGTTTTGAGTCTTAAACAGATCACATTTTGTT10.684931506849315No Hit
CTTGGTTTCTCCACTGCTTCCTGCAGTGGCAGGTGTCCCCCGGTCCTTGGT10.684931506849315No Hit
GAGGATTCCGATTGAGCTAAATGTTTAAGGGTAGTAGAGTTTTTGATGTCT10.684931506849315No Hit
GGCATTACCCCTTTCGGAAGGTTATGATAAACTGCTTCTTGTGTGTTCATT10.684931506849315No Hit
GAATGGATTCAACTTGAATGGAATGGAAAGAATGGAATCAACACTGTCTCT10.684931506849315No Hit
CACTAAGTGTGCGGAAAACAAGGGAATAGTTGAACACTTTTAAGAGTAGAA10.684931506849315No Hit
AGACAAGAAAGATCGCATTTTTTTGACTTCGTAAAAATAAGAGTTGTACCC10.684931506849315No Hit
ACATCAGCCAGGGAAGAGTTTTAAGAGATGGCATCAAAGTTGCTACAATTG10.684931506849315No Hit
CCATTTGATGTTGATTCCATTCTATTTCATTCGATGATTCTATTCGAATCC10.684931506849315No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.684931506849315No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA10.684931506849315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT10.684931506849315No Hit
GCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGTAGTCTGGAGAAGCGTC10.684931506849315No Hit
CACTGTAATTAAGTAGTGTGGTTAGAGCACAGAGCTTAGCTAATAACCTGT10.684931506849315No Hit
CAAGTAGGATATAGTGATCCGATGATTCAGAATGGAATGATCCTGTCTCTT10.684931506849315No Hit
GAAGGTAACTAGATTGTGTTCTTCAAGAACTGTACAACAACTGTCTCTTAT10.684931506849315No Hit
TTGTAAGACCACGACTGATCCTGAAAGGAATGAACGGAAAAAATAGCATGT10.684931506849315No Hit
GGTTAATCAAAGAGGGTAATAATCCCGTAGACGAAATCTGTCTCTTATAAC10.684931506849315No Hit
CAAAAATGACAGAAGGGAAAAGACAAATTGTATCTGTCTCTTATAAACAAC10.684931506849315No Hit
GAGAGGTATGATAAGGAAGAAGATGAAGTAGAGGAGCTGTCTCTTATACAC10.684931506849315No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCTGTCTCTTATACACATC10.684931506849315No Hit
GAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAA10.684931506849315No Hit
GCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTC10.684931506849315No Hit
ATAGAAACGAGGAAGAAGATTTCAATACACCCTGTCTCTTATACACATCTC10.684931506849315No Hit
ATGTTATAGATATAAGCATTTGACTCATATCACACCTCCTGTCTCTTATAC10.684931506849315No Hit
AAATAGAGGAAGGAAAAAAGGAAAAACACAAGAGAGTGACTGTCTCTTATA10.684931506849315No Hit
GAGGAAAAGACATCAATAGAGATTCCCCTAGTAGCTGTCTCTTATACACAT10.684931506849315No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCTGTATTACCGCGGCTGCTGGCA10.684931506849315No Hit
CATTAGTACTCACATGCTAGAGGTGAAATTCTTGGATTTTGTGAAGACTAA10.684931506849315No Hit
ATCAACGCAGAGTACGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGAG10.684931506849315No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACG10.684931506849315No Hit
ATCCACAACATCCAAAAACAACTTCCAAATTCCAAAAAAAAAGCAGTATTC10.684931506849315No Hit
GTACGGGCCATGATCGGCGGCGTTTCCGGGATCGCCGCATCGCCTCCTGCC10.684931506849315No Hit
GTCTACACCTTTCGTTTCTGGGTTCTTTTGAGAGTTGAAGCTTCAATCAAA10.684931506849315No Hit
CTACATAGGACTGTGGGTCTGCGTCGTGCTGTGCTGTGCCAGGCTATGCTG10.684931506849315No Hit
GATCTATCCCCATCACGATGAAATTTCAAAGATTACCCCTGTCTCTTATAC10.684931506849315No Hit
ATTCCATTCCATTCCATTTCATTCCATTGCAATCCATACCTGTCTCTTATA10.684931506849315No Hit
CCATCATCAAAGACCAAAGGTAGATAAAATCACAAAGATGGGGAGAAACCA10.684931506849315No Hit
ATCTATTCCATTGAATTTCATTCCGTTCTGTTCCATTCCATTCCATTGCAT10.684931506849315No Hit
CATAAATTCAGACCGAGAAAACATAAAAATTTGAGTCTTTTGATGATCGGA10.684931506849315No Hit
GTCTAAGGGAAGAAAATGACAGAAGAGAAAGAGTAGCTGTTGAGACTGTCT10.684931506849315No Hit
TTATCCTCCCTTACAACACTCTACTTTTACTCTGTCTCTTATACACATCTC10.684931506849315No Hit
GCTTTATCCATGTATGGAAGATTGATATGGGAAACGAAGAGAGATGAGACT10.684931506849315No Hit
GGTGTAGGCGCTTTCCAAAGGTGGAATCTTCTAGTTCTTCCTATTTGACCC10.684931506849315No Hit
GAGTCCTGTAGGCGAACGAGCGGTTGACTGTACTGCTGTCTCTTAAAATCT10.684931506849315No Hit
GAAATAAACGCCATGAATAAGGTTATGTGTTGTACGCTATACCCCCTGTCT10.684931506849315No Hit
CTTTTATGGTTCATATTCAGGATTAGGTTCATCCCTGTAGAAATTGGATGA10.684931506849315No Hit
CAAGAGAACAGGGAAAAACCAAAAATACCTCAAGGTCAAGGATATAGATAA10.684931506849315No Hit
GGTATTCAACACAGAGTAACCAAGGATGATGCAATGAAATCTGTCTCTTAT10.684931506849315No Hit
ATATAAAACAGTAAGTATCTTGCAACAACAGTGATGTTTCTGTCTCTTATA10.684931506849315No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC10.684931506849315RNA PCR Primer, Index 23 (95% over 21bp)
GTGGTGTAGAACTTAAAAAAGATGTTCCAATACCATTCAAATACATGAAAA10.684931506849315No Hit
GTATTGGGTTGGCTCAAAACATGCCAAGACAAACTACTACTGACAAGCTCC10.684931506849315No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACCTGTCTCT10.684931506849315No Hit
GTACATAGACTATGGTATCATCATTATTAACAGTGCGTGTAGCAACTGTCT10.684931506849315No Hit
ATATCTGAATGGGGAAACCCACTTGTCATAAGACAGGTATCGTAACTGTCT10.684931506849315No Hit
TACTACTTCTACTGTGTCCCCTACTTTTACTACTCCTTGTTCTACTCTTCC10.684931506849315No Hit
GGTACATATTAATAAAAGGAATCTCAGCACCCGTACTCTGCGTTGATACTG10.684931506849315No Hit
GTCACAATGTATAGCATGATCTCCATGATTCATACCACGATAACTTATGTA10.684931506849315No Hit
GATTATAATTTAGAGGAATGACTTGGAAAGGTCAATCGTAGAGCTGTCTCT10.684931506849315No Hit
GTGTGTGTCATCCAAACAAACAAAATTTAAACATAGGTATCGATCAATGAC10.684931506849315No Hit
CTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTGGG10.684931506849315No Hit
ATTCCATTCCATTCCATTCCATTCCGGATGATTCCATTCCATTGCATTCCG10.684931506849315No Hit
AGGGAGAGAGAATTCTGAGTTTGTTGTTCACAAGATTACTACTCTTACTGT10.684931506849315No Hit
GGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCG10.684931506849315No Hit
ATTCGCAGTTTCACAGTACAAAGTTGTTTATACTTAAACATGCATGGCTTA10.684931506849315No Hit
TCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTAC10.684931506849315No Hit
AAACAAAACTCAAGCTTTTGTAATGTCTAATATTCAGTCAACAAACTTATA10.684931506849315No Hit
TCCTTATCCTTGATATCCTTATCTTTGCTATGATTGCTGTCTCTTATAACA10.684931506849315No Hit
TCTCTGTTCTATTGTCAATATGTACAATACCCATGTACATTCTGTTGTGCC10.684931506849315No Hit
CTTTAAAAGCAAGAGGAAGTGATCTGATTCGATAACTCTCAGCAACACAGC10.684931506849315No Hit
AAGCAATGACAGGTGATGTGAACATTTACATGAATGACTGTCTCTTATACA10.684931506849315No Hit
CATCTCGCCCGCCCGACTCATAGCTGAAAGAGTGGAGTTCGCTCGTTTATT10.684931506849315No Hit
GCTCTTCATGATGATCTCTTCCATGACTTCCTTCTTACTCTTACGCCTCTC10.684931506849315No Hit
CTTATGCATGGTTAAGCCATAATACTGATGTGGCTGTCTCTTATACACATC10.684931506849315No Hit
ACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGA10.684931506849315No Hit
TTCATAAGGCATCATATTTAATTATCGATGAAGCAGATTCTGTCTCTTATA10.684931506849315No Hit
TCTGAATAGTGCTTCTGGTATTCACAGAGATCTGTTAGATTCTGTTGTGTC10.684931506849315No Hit
AGTGATCTACCGAAGCTATGGATTCAACTCGCGAAGCGAGTTGTCTGGTAG10.684931506849315No Hit
CATCCCTAATTCCAATCATACATCTTCCGCATACGGTGTATCAAAGTACTG10.684931506849315No Hit
CTTCTAAGCCAACCTCCTGGCTGTCTATGCGACGACACATCCTTAACCACT10.684931506849315No Hit
ATGGAATGGAATGGAATGGAATCAACCCAAGTTGAATGGATTCGAATGGAG10.684931506849315No Hit
ATCCTGATTCCATTCGTGTCCATTCGATGATTCCATTCTACTCCATTCGAT10.684931506849315No Hit
CTGTTTTAGGCCGAAATATAGAGCAAACTATTTTCTCACTCATTTCTGTCT10.684931506849315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTCTGG10.684931506849315No Hit
CTACAATGATTTCAAATTCTTTCCCAAGTTGAAGAGAAAAATAACAATAAA10.684931506849315No Hit
TAGCAAGAATGATCTTAAAAACGTCGGAACCAGTCAAAGGCTGTCTCTTAT10.684931506849315No Hit
CATACAGATTGAACAGCTAAACAGAACGAAAAAGCATGCACATCTTACAGA10.684931506849315No Hit
GTTGGAGAAGCCACCTGTTGCCAGTGGGCAACCCAAGAGAGAGAGTAACCT10.684931506849315No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTCTGTCTCTTATACACA10.684931506849315No Hit
GGAAAGACACTGTACAGTCAAAGATCCAAGATTCACTACATAGTACCTGTC10.684931506849315No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCCT10.684931506849315No Hit
CATGTTGGCCACGATGGCCGGCGCCAGGCCCTTGCTGGGCTCGTCGATGAC10.684931506849315No Hit
CATCTGTTTCCAGCTTCTGGCTTTTATAATTAAGGCTGCTATTGGAACATG10.684931506849315No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGCTGTCTCTTA10.684931506849315No Hit
CTCCTGACCACAATGACAATACAGTTATCAATAGCTATAAACTGTCAGAGG10.684931506849315No Hit
GTATTAAACCTTCTTACTCATATTCATGTGACTCGGCCATGTATCATTTGG10.684931506849315No Hit
CTATTATGATGCATCAAAAAGAATGCCACAAGAACTAATTGAGGCTTCAAA10.684931506849315No Hit
ACATAAAACAGAACGGACGCTTAATTGTATGAAATTAATGCTGTCTCTTAT10.684931506849315No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCTGT10.684931506849315No Hit
CCCTGGGAGGATTAGCCTAGCCCAGGAACCCTTGGTCATCCGGAGGACATG10.684931506849315No Hit
CTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCTGAGGACGC10.684931506849315No Hit
AGCATATGCAGATCAATGATCAGTCAAATCATCTTCTCCAAGAACGAGATT10.684931506849315No Hit
AATGGAATGGAATGAAATCAACCCGAATGGAATGGAATGGAATGGAATGGA10.684931506849315No Hit
CCCCAGGGCCACGCCCAGCGCCCTCACCTCGTCCTTGAACCTGTCTCTTAT10.684931506849315No Hit
GTCATATTCACCACTGGTAGTGCTACAAATCATGTTTGTTCTTGTATTTTA10.684931506849315No Hit
GTCGTATCAAGGGAGAATTCAGATAACATTTTTTTTGCTTTCCTGATCCTG10.684931506849315No Hit
GATAACAGGCTGATAACCCCCAAGAGCTCATATCGACGGGGTTGTTTGGCA10.684931506849315No Hit
GAGTAACGATAAAAGAAGTGAGAATCTTCTTCGCCGTAAGGACTGTCTCTT10.684931506849315No Hit
TGTCTCGCCGGCCGCCTCAAGCGCCTTCTTAGCTGCCTTCTTCTCCTTCTT10.684931506849315No Hit
ATATTGTTGCAGCTGTCTTAATGAATGCCTCCTCAACATTCTGAGCAGTCT10.684931506849315No Hit
AAGCTTTGTAATATGATGAATATCATGTTGGTAGGAGAGCGTTCTGTAAGC10.684931506849315No Hit
ACACAAGAGATATTATAAATCCTTACAATCAAGAAACAATCTTCACTGTAG10.684931506849315No Hit
AAAGTCTTCAAGTGCCAAAACTGAAGACCTGCGACCGATAAACAAACTGCA10.684931506849315No Hit
GTCTTACTGTCTAGCCGTTGGTACTCCGCATCTTCACGGAGAATTCAATTT10.684931506849315No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACTCTCCGAG10.684931506849315No Hit
CCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCC10.684931506849315No Hit
GTTGTAAGCTGCAAAGAAAAGAAAAATCAGGATACGATTCAGGATGCAGAA10.684931506849315No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACGCTTT10.684931506849315No Hit
TATGCGTACCAACTCGTTTATTTGTCTTCAATTTTAAATACCTGTCTCTTA10.684931506849315No Hit
ATACATAGACTTGGATTATAGCTACTGCAAACTCTAGAATTGTTAGTAGGA10.684931506849315No Hit
CTTCCTTTTTTCTTTGTGAGTTTCGAGAATATCCCTATTCATAGGTCCTGT10.684931506849315No Hit
ATACTAAGTTGAGATGATATCATTTACGGGGGAAAGCGCTTTGTGAAGTAG10.684931506849315No Hit
TCTCCGCTGTTACAACGCTCTCCTACCATCCTAAAAAGGATCCACAGCTTC10.684931506849315No Hit
GGGTAATCCCGCCTGACCTGGGGTCGCCCCGTACTCTGCGTTGATACTGTC10.684931506849315No Hit
ATATTTCACTTCACCGAGTATTGTCAACTTTGTATTCTCCCTGTCTCTTAT10.684931506849315No Hit
TTATGTAACTAGCAACTCTGGCAAAGGAAAGATTTGATTGAGAAATAGTAT10.684931506849315No Hit
AAAAATAGCATGTCGTATCAAGGGAGAATTCAGATAACACTGTCTCTTATA10.684931506849315No Hit
GTTGTATGTTATATGACAACACACAACAAAGGGGTGTACTCCTTTCCTTCA10.684931506849315No Hit
GTTGTGTTTAGGAGTGAAAAGCTAACCGAACCTGGAGATAGCTAGTTCTCC10.684931506849315No Hit
GTAAAATGGGACTGTCAAAATGGCAGCCTTAGTCGTCTTCTAAGTAGTGAT10.684931506849315No Hit
GTGCTGCGAGGCGCGTGCGCTGCGCGACCATCGCACGCGACTGTCTCTTAT10.684931506849315No Hit
AAACGTTGAAACATGATTGCCCGTACTCTGCGTTGATACCTGTCTCTTATA10.684931506849315No Hit
ATATTCGTCTGAATAAAGTTGATCGGAAGATGTATCTTCATCGGGTTGATT10.684931506849315No Hit
TTCTAAAACCTGTTCCGAGAACTTGTATAGAGAAGGATCTGTCTCTTATAA10.684931506849315No Hit
GCCTTAAATCTACTAGTGTTGCCTTGTATTTTTCACTTGTCTTCTATCCTG10.684931506849315No Hit
TCTCAGTACCTGAAGGAGAAGAAAGAGAATTCTGTCTCTTATAACATCTCC10.684931506849315No Hit
CTTAAAGTAAGATAACCAACAAGAGTTAACTGGAACAAAACTAAATAACAT10.684931506849315No Hit
AGTTAATTCTCTGCTTTAAAAACTCAAAACAGTAAGCAGAACAAATCCTGT10.684931506849315No Hit
CTATATTCCATGCCATTGCTTTCCATTGCATTCCATTCCTTTCTATTACCT10.684931506849315No Hit
CTCTATCATTTTGAATTGAATACTGCTTTTTTTTTGGAATTTGGAAGTTGT10.684931506849315No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers