FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_22.3410000000ae47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_22.3410000000ae47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902510
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC31200.3457025406920699No Hit
AGATTGTACCGATCAGGAAAGCAAAAAAAAATGTTATCTGAATTCTCCCTT20210.22393103677521578No Hit
AAATAAGATTGTACCGATCAGGAAAGCAAAAAAAAATGTTATCTGAATTCT19950.22105018226944856No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA9510.10537279365325591No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4150.040.660945
GTGCCTC751.8189894E-1235.9984511
ATGCCGT4700.035.42400444
CCACGAG5000.034.19852420
CCCACGA5100.033.52796619
GCCCACG5250.032.57002618
AGCCCAC5300.032.26276417
CCCTCGA352.820613E-432.1414723
CGTATGC5200.032.0178541
CTGGACC5150.031.89182930
ACGAGAC5450.031.78762222
GGACCAT5200.031.15250232
TATGCCG5350.031.12015343
GTATGCC5450.030.9619742
CACGAGA5650.030.66239521
GAGCCCA5600.030.53439916
CGAGACT5550.030.40409523
TCGTATG5500.030.27142540
TCACGAT300.00514776429.99870720
GAGACTC5600.029.73086424
Produced by FastQC (version 0.11.2)