FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_20.3410000000ae2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_20.3410000000ae2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC21.9047619047619049RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC21.9047619047619049No Hit
GTGCATAAGTTAACTTCCAATTTACATACTCTGCTTAGAATCTGTCTCTTA10.9523809523809524No Hit
ATGTTACAAAAAGTGATAGGATCTCTCTGTCTCTTTAAGGGTATCTCTGTC10.9523809523809524No Hit
GACTAGAAGGTAAACACTACGATATGGTAGATGGCAAACCAGTTCCTAAAA10.9523809523809524No Hit
GTCCTCGTCAGCATCGCCGTCCTCGCCCAGTTCTCGAGAAGAAAAAGAGAG10.9523809523809524No Hit
TCTTATACACACTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC10.9523809523809524No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG10.9523809523809524No Hit
AAACATCTCAGTAACCCGAGGAAAAGACATCAATAGCTGTCTCTTATACAC10.9523809523809524No Hit
TCCATACGCTATGTATTTACGTATGGATACTAGAGTGTGTCTAGTAGGTTT10.9523809523809524No Hit
TTATTTTACTTTTCAAGGCGATGCCTAAGTTAACAACAATGTGTTTAAAAG10.9523809523809524No Hit
ACCCAACACTACAAAATGCGTTAAAGTTCTCTCATAAGAAGCAGAACTTCA10.9523809523809524No Hit
TCCTAAGGCTAAATACTCTCTAGTGACCGATAGTGAACCAGTACCTGTCTC10.9523809523809524No Hit
GCATTGGACAGAGTTCACAACTTATACAAATACTTTTAGTCCTGTCTCTTA10.9523809523809524No Hit
GAATGGTACAGTGATGATCAACATTTACAGAGACGTATGCCAGTATGAATA10.9523809523809524No Hit
TACTAAGATCGACTGGAACAAGATCCTTAGCTACAAGATTGGCAAAGAGAT10.9523809523809524No Hit
GAGTGAGGCTGCGAGAGCTAACTTCCGTAGCCGAGAGGGAAACAACCCAGA10.9523809523809524No Hit
GAATTAGGGTACTTGAAATATCTTTAGTTATGGTTGATTGATTCTATCATC10.9523809523809524No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG10.9523809523809524No Hit
TGATATGACATTGTAAACTCCAATTGCTTCGCGCAATGTTATCTATTCACA10.9523809523809524No Hit
GTTTTATTCGTCTTTTCAAAGTTGGAGCAGAGTTGTTATTTTTAGCCAAAG10.9523809523809524No Hit
TTTTTGTACAATTTACAAACACATAGTATACAAGCAAACTCTGATTGGTAT10.9523809523809524No Hit
TGCTTTCTCTTCTTCTTATTCTTCTTCTTCTCCTCCTTTGCAACCTCTACT10.9523809523809524No Hit
GATTCTTGCAGTTACAATTGTGTGGAGAAACTAAACCACATTGATCAAAAG10.9523809523809524No Hit
CCTATGTATTGTCATCTTCCATTTAACTGTCAATACCTAAAGTCTAAATGC10.9523809523809524No Hit
TCATCATACCTTGCTTTTCCTAATTTATCTGTCTCCTGTCTCTTATACACA10.9523809523809524No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA10.9523809523809524No Hit
GTTCTTTTTAGTGCTGAATAATATCCAGTATCTGACATCTGTCTCTTATAC10.9523809523809524No Hit
GAGTACGGGCTGGTAACCAAAAAAAATTGCTATTTTTGTCATTAATCTGTC10.9523809523809524No Hit
CTTTTGTTACAATAGAAACAGAAGTGATGTGGATTATTCAAGAATCGAAGT10.9523809523809524No Hit
TTTCAAGACTGGGAAGAACAGGTGGTTCTTTACTAAGCTCAGGTTCTGAAG10.9523809523809524No Hit
GACTATGCACGAAAGGGTAACAAGAGCCAGAGACATGTCGCTAAACCTAGC10.9523809523809524No Hit
GGAGAACATTGATTAAGGAAGCATTTTCCTGATTGATAAAAAAAATAACCT10.9523809523809524No Hit
GTTTAATACGACGTGAGTCAGTATAGATTCTATCCTCGGAAGAAAACAAGA10.9523809523809524No Hit
TTCCAGTAGATGGACAAATTGAATTGTCTTGGAGAAGTGGCTCAACTATTG10.9523809523809524No Hit
TCTTATACCATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC10.9523809523809524No Hit
CTCCTTATAGAGTTAAAACAGAACTTTCTTATCACTGTCTCTTATACACAT10.9523809523809524No Hit
ATATATGGCCAAGAACGAAGGAACAAAATCATTTCAGAGCTTAAACCTGTC10.9523809523809524No Hit
GTATGAGATCAATCTGATCCTTTATTGGTTATAAAAATGGCAAAGCAAATA10.9523809523809524No Hit
CTCTATCTCTTTTACTTGCTTTTTTTTGATCTGTTAAGACTTTTTATGCTG10.9523809523809524No Hit
GGTTCTCAGAAGACCACGGAGAACATAGAGGAACTTGGTAAGGCCTGTCTC10.9523809523809524No Hit
CTAATAATCAAATGGAATGAGAAATGAAGCCACAAAAGTATCTGCAATCAA10.9523809523809524No Hit
AGCATATGCTGCTGAACAAGAAACAGTAGAAAAAGAATATGATGCTCAAGA10.9523809523809524No Hit
GAGAAGTGAAACATCTCAGTAACCCGAGGAAAAGACATCAATAGCTGTCTC10.9523809523809524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTATACCT10.9523809523809524No Hit
GGTTCGAGTTTCGGAATATAGATATCATCAATCAAAATACCTCGATGCGAC10.9523809523809524No Hit
CATCGAGAGTTACTTACAAGAATGTGCCTAACTGGCATAGAGATCTGGTAC10.9523809523809524No Hit
GTAGTAACAGAAACAACATGAGAATCAAACAAAGAAGCTAGGCACTGTCTC10.9523809523809524No Hit
ATCTCATTAAAAATGGGCAAAGGGACTTGAATAGACCTGTCTCTTATACAC10.9523809523809524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCACA10.9523809523809524No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC10.9523809523809524RNA PCR Primer, Index 45 (95% over 21bp)
GCCTTCTGACATGCTATGAATTCACATGTCGATAACATGACATAACTCATG10.9523809523809524No Hit
TTAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC10.9523809523809524No Hit
GGTATCGGCACATACGAATTGATCCGTAGATCTCCGAATGGGGCAACCCGG10.9523809523809524No Hit
CTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCGA10.9523809523809524No Hit
AGATTCTTCTTTGCATAGCATAAGATACCCAAATATCTGTCTCTTATACAC10.9523809523809524No Hit
TCCAAATAACAAGCCAGAATATACAGGCAAATCCCTGTCTCTTATACACAT10.9523809523809524No Hit
CTTTAGTTCTTTCCATCTTCTTCCTTAAATAAATCGCTCTAAACCTGTCTC10.9523809523809524No Hit
ACCTAATACAGAATGCTTCGGATAGAGAAAGCCAACTTTTCAGGATTACTT10.9523809523809524No Hit
ATTACGCACTCTTTAAATGATGGCTGCTTCTAAGCCAACATCCTAGTCTGT10.9523809523809524No Hit
AAATTAATCATTTTGACTAACGGTTTTTACGTAAATTATAAGCTGTCTCTT10.9523809523809524No Hit
TATCAAGGGAGAATTCAGATAACATTTTTTTTTGCTTTCCTGATCGGTACA10.9523809523809524No Hit
GAGTTCTAAAGCACAAGAAGATAGAACTAATAGCTGTCTCTTATACACCAT10.9523809523809524No Hit
GTATCAACGCAGAGTACGGGGGTGGTATCCTGTCTCTTATACACATCTCCG10.9523809523809524No Hit
AACTGAAACAGCTCAGTACCTGAAGGAGAAGCTGTCTCTTATAACATCTCC10.9523809523809524No Hit
CAGTAAAAGAACTTCTTGAGGAAATCACCATCACTGACCTCAAGCTGTATT10.9523809523809524No Hit
GAGTACGGGACTATAAAAAGAGAGAAAACAAGCTTTGTTATAGCTGTCTCT10.9523809523809524No Hit
ATAAAGATTCGAAAAACAAAACTCGAAAAATCTGATCAAATGTTGTTGTTA10.9523809523809524No Hit
CTTCGTCGCCGTAGTAAATAGGAGAGAAAATCGAATTTCTTTCTTCGTCTT10.9523809523809524No Hit
CTCCTTCCGGTATGGCTTCTCCGCGGACCTGTCTCTTATACACATCTCCGA10.9523809523809524No Hit
CTTAGTAACTGATTAGGTCAAAAAACTCGTTTGTATTGTACTCTTTCGCGT10.9523809523809524No Hit
ATTTGATACATGCCTCATGTTTGTGGTATACAGTAGGAAAGGCTTTTCAGG10.9523809523809524No Hit
CTTCTTGTGTATATCTTTCATGTGCAATGTAAACACCATTCTTATCCCTTG10.9523809523809524No Hit
GTCCTCCCTCATCATTATGTTTGTTTCAGATACCAAGAACTAACTGACCTG10.9523809523809524No Hit
GGAAAGGTGTGAATCTCAGATCCATCATACTTATAGATACCTACTACCTAC10.9523809523809524No Hit
GAGTATGAGTGTTTGCCTACATGTATGTATGTGTGCTAGCTACTCATGCTG10.9523809523809524No Hit
CGATAAAAGAAGTGAGAATCTTCTTCGCCGTAAGGACAAGGGTTCCTGGGG10.9523809523809524No Hit
ACTCCGGGCCCTGCGGAAATTAAGCAAGTGGGATTGAAGACTGACGCCGAT10.9523809523809524No Hit
GTCCATCATATATTTTTTTGCTAAGAAGAAACCCTGTCTCTTATAGACATC10.9523809523809524No Hit
CCTGGGATACCCATTAATAAGTAAATACACAGCAGTGCCCGTCTCATATAC10.9523809523809524No Hit
ATTACAATTAAGATAATTGCTAACCATGTTGCCATTGTTTCACCCACTTTC10.9523809523809524No Hit
ATGTAAGAATGGTACAGTGATGATCAACATTTACAGAGACGTATGCCAGTA10.9523809523809524No Hit
GAGTACATCATGCAAATATCCACACATTCCAGGAGCTGTCTCTTATACACA10.9523809523809524No Hit
ACCTAAGACTGTGGAAACACAATCACCAACAGGAAAAGAGTATACTACCTG10.9523809523809524No Hit
GAATATTGTGGAGCCACCATATTATCTCTGTTTAACTTCTGTTCTGTTGAA10.9523809523809524No Hit
CACTATTACAGTATCATTGACTCTTCCTTATGCCTTTTATTCCTGTGACTT10.9523809523809524No Hit
ACATCTTAGTACCCGTAGGAAGAGAAAACAACCGTGATTCCGTTAGTAGCT10.9523809523809524No Hit
ATTATCTGTTTCCTCCTTACTGTCATCATCATTTAGTCTGTTGTTACTGTC10.9523809523809524No Hit
ATATAAGAGTAAACTGTAGTAGTGTCGAAATGACAAAACCTGTCTCTTATA10.9523809523809524No Hit
TTCTTCTATCAACTTGATAAAAGTGATATGATGGATTACGATAAGTCGTCA10.9523809523809524No Hit
CAATAAAGACTTCATGTCTCAAGAATTGTGTTCCCTTATCCTACTGGCTTA10.9523809523809524No Hit
ATCTAGCTATGTGGTCCACTACTTGTAATCTACTTACATCTGTCTCTTATA10.9523809523809524No Hit
GAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATCTGTCTCTTTAT10.9523809523809524No Hit
CACTGTGACTCCCACAACCCCCATGATGCAACGCCTAACCGCTATCACACA10.9523809523809524No Hit
ATTTAATATCGTCGATTCAAGTGCAGCCATGTAACTGTCTCTTATACACAT10.9523809523809524No Hit
CACTTGTATATAATCCACAATTGCTTACGCTTTTTGCTTCAATTCATATAC10.9523809523809524No Hit
GTGTATACCCTAACATCGTTCAAATACGCGGTACAAGTCCTGTCTCTTATA10.9523809523809524No Hit
CTCTATTGATGTCTTTTCCTCGGGTTACTGAGATGTTTCACTTCTCCCGGT10.9523809523809524No Hit
GTGGTATCAACTCAGAGTACGGGAAAAAAAACTGTCTCTTATACGCATCTC10.9523809523809524No Hit
GTGTATGTGGGAATTTGGAGGTTTTGTAAAACCCTTTTCTCCAAGCCCATG10.9523809523809524No Hit
GACTAGTGTTGCAAAACTAGCGGATGAGCTGTGGATAGGGGTGAAAGGCTA10.9523809523809524No Hit
GTACATGCCAGATTTGGTGTTTTCTTCAAATGTTTATGTGCTGTCTCTTAT10.9523809523809524No Hit
CTTCAATGCTGTGATTCTGAAAAGGCTTTTCATGAGCAAAGTCAACAAAGC10.9523809523809524No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers