FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_2.3410000000ac49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_2.3410000000ac49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10
Sequences flagged as poor quality0
Sequence length51
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGA110.0No Hit
GAACAAGAACAATCCATACAAGATCAGACATTCGTCATATTACATATCTGT110.0No Hit
GCCTTATACTACATAACGAGGCTGGAAGTTTACCTATATATGCCCTGTCTC110.0No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATAACAAAGAAC110.0Illumina PCR Primer Index 5 (96% over 28bp)
GAATAAAAGGTCTTCAAGGGAAGAAGTATCTTCAGATGATAATGGCAGTTC110.0No Hit
GAATGAAACAAACATTCATCTCTTCAAGCTTCTATGACTGGGTACTCGATA110.0No Hit
GTCTATCTCTACTTCATCCCTAACAAAGATATAGGCCATCAAAAGAGAACA110.0No Hit
TCCGTAAGGTTACTAAGGAAGAGGAAAGCATAAGAGAGCTTTCTTTTGTCT110.0No Hit
GTCAAATTCAAAAAGATGTAACATCTCTTCTTTGGCTGTCTCTTATACAAT110.0No Hit
TATCAATGACAGACAAGATGTCATTACAAACTTTAGCACTGTCTCTTATAC110.0No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)