##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_4_2.3410000000ac49.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10 Sequences flagged as poor quality 0 Sequence length 51 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.0 0.0 0.0 0.0 0.0 0.0 2 34.5 0.0 0.0 0.0 0.0 0.0 3 35.5 0.0 0.0 0.0 0.0 0.0 4 37.4 0.0 0.0 0.0 0.0 0.0 5 36.2 0.0 0.0 0.0 0.0 0.0 6 37.6 0.0 0.0 0.0 0.0 0.0 7 38.2 0.0 0.0 0.0 0.0 0.0 8 35.5 0.0 0.0 0.0 0.0 0.0 9 37.4 0.0 0.0 0.0 0.0 0.0 10 38.0 0.0 0.0 0.0 0.0 0.0 11 35.5 0.0 0.0 0.0 0.0 0.0 12 38.1 0.0 0.0 0.0 0.0 0.0 13 37.9 0.0 0.0 0.0 0.0 0.0 14 35.9 0.0 0.0 0.0 0.0 0.0 15 35.3 0.0 0.0 0.0 0.0 0.0 16 35.4 0.0 0.0 0.0 0.0 0.0 17 37.8 0.0 0.0 0.0 0.0 0.0 18 37.4 0.0 0.0 0.0 0.0 0.0 19 38.2 0.0 0.0 0.0 0.0 0.0 20 37.8 0.0 0.0 0.0 0.0 0.0 21 36.1 0.0 0.0 0.0 0.0 0.0 22 35.3 0.0 0.0 0.0 0.0 0.0 23 36.3 0.0 0.0 0.0 0.0 0.0 24 35.1 0.0 0.0 0.0 0.0 0.0 25 36.8 0.0 0.0 0.0 0.0 0.0 26 35.7 0.0 0.0 0.0 0.0 0.0 27 39.0 0.0 0.0 0.0 0.0 0.0 28 35.9 0.0 0.0 0.0 0.0 0.0 29 34.6 0.0 0.0 0.0 0.0 0.0 30 35.0 0.0 0.0 0.0 0.0 0.0 31 35.9 0.0 0.0 0.0 0.0 0.0 32 37.1 0.0 0.0 0.0 0.0 0.0 33 36.1 0.0 0.0 0.0 0.0 0.0 34 38.6 0.0 0.0 0.0 0.0 0.0 35 38.6 0.0 0.0 0.0 0.0 0.0 36 37.6 0.0 0.0 0.0 0.0 0.0 37 37.9 0.0 0.0 0.0 0.0 0.0 38 37.6 0.0 0.0 0.0 0.0 0.0 39 34.4 0.0 0.0 0.0 0.0 0.0 40 37.2 0.0 0.0 0.0 0.0 0.0 41 34.4 0.0 0.0 0.0 0.0 0.0 42 31.9 0.0 0.0 0.0 0.0 0.0 43 34.6 0.0 0.0 0.0 0.0 0.0 44 32.7 0.0 0.0 0.0 0.0 0.0 45 34.2 0.0 0.0 0.0 0.0 0.0 46 34.6 0.0 0.0 0.0 0.0 0.0 47 34.9 0.0 0.0 0.0 0.0 0.0 48 34.7 0.0 0.0 0.0 0.0 0.0 49 37.7 0.0 0.0 0.0 0.0 0.0 50 35.4 0.0 0.0 0.0 0.0 0.0 51 35.2 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2209 1 0.0 2209 2 0.0 2209 3 0.0 2209 4 0.0 2209 5 0.0 2209 6 0.0 2209 7 0.0 2209 8 0.0 2209 9 0.0 2209 10 0.0 2209 11 0.0 2209 12 0.0 2209 13 0.0 2209 14 0.0 2209 15 0.0 2209 16 0.0 2209 17 0.0 2209 18 0.0 2209 19 0.0 2209 20 0.0 2209 21 0.0 2209 22 0.0 2209 23 0.0 2209 24 0.0 2209 25 0.0 2209 26 0.0 2209 27 0.0 2209 28 0.0 2209 29 0.0 2209 30 0.0 2209 31 0.0 2209 32 0.0 2209 33 0.0 2209 34 0.0 2209 35 0.0 2209 36 0.0 2209 37 0.0 2209 38 0.0 2209 39 0.0 2209 40 0.0 2209 41 0.0 2209 42 0.0 2209 43 0.0 2209 44 0.0 2209 45 0.0 2209 46 0.0 2209 47 0.0 2209 48 0.0 2209 49 0.0 2209 50 0.0 2209 51 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 25 1.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 1.0 33 0.0 34 1.0 35 0.0 36 1.0 37 1.0 38 1.0 39 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 70.0 0.0 20.0 10.0 2 0.0 50.0 30.0 20.0 3 0.0 30.0 20.0 50.0 4 20.0 10.0 40.0 30.0 5 10.0 50.0 40.0 0.0 6 10.0 70.0 20.0 0.0 7 10.0 50.0 30.0 10.0 8 30.0 40.0 30.0 0.0 9 20.0 20.0 0.0 60.0 10 20.0 20.0 40.0 20.0 11 10.0 60.0 20.0 10.0 12 20.0 50.0 0.0 30.0 13 0.0 30.0 50.0 20.0 14 0.0 30.0 50.0 20.0 15 10.0 30.0 30.0 30.0 16 10.0 70.0 10.0 10.0 17 30.0 10.0 30.0 30.0 18 40.0 40.0 0.0 20.0 19 20.0 20.0 30.0 30.0 20 30.0 30.0 20.0 20.0 21 20.0 30.0 40.0 10.0 22 10.0 30.0 10.0 50.0 23 20.0 40.0 30.0 10.0 24 20.0 20.0 40.0 20.0 25 10.0 30.0 30.0 30.0 26 30.0 60.0 10.0 0.0 27 10.0 50.0 10.0 30.0 28 40.0 30.0 20.0 10.0 29 0.0 20.0 40.0 40.0 30 10.0 40.0 30.0 20.0 31 0.0 20.0 60.0 20.0 32 0.0 30.0 50.0 20.0 33 10.0 30.0 30.0 30.0 34 40.0 30.0 20.0 10.0 35 30.0 30.0 30.0 10.0 36 30.0 30.0 0.0 40.0 37 0.0 40.0 40.0 20.0 38 40.0 40.0 10.0 10.0 39 0.0 30.0 40.0 30.0 40 10.0 20.0 50.0 20.0 41 30.0 20.0 50.0 0.0 42 20.0 40.0 30.0 10.0 43 0.0 20.0 30.0 50.0 44 10.0 30.0 40.0 20.0 45 20.0 20.0 30.0 30.0 46 0.0 40.0 50.0 10.0 47 20.0 40.0 30.0 10.0 48 40.0 20.0 20.0 20.0 49 0.0 40.0 40.0 20.0 50 20.0 30.0 30.0 20.0 51 0.0 30.0 30.0 40.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.5 31 1.0 32 1.5 33 2.0 34 1.5 35 1.0 36 2.0 37 3.0 38 2.0 39 1.0 40 0.5 41 0.0 42 0.5 43 1.0 44 0.5 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.5 51 1.0 52 0.5 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 10.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGA 1 10.0 No Hit GAACAAGAACAATCCATACAAGATCAGACATTCGTCATATTACATATCTGT 1 10.0 No Hit GCCTTATACTACATAACGAGGCTGGAAGTTTACCTATATATGCCCTGTCTC 1 10.0 No Hit GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATAACAAAGAAC 1 10.0 Illumina PCR Primer Index 5 (96% over 28bp) GAATAAAAGGTCTTCAAGGGAAGAAGTATCTTCAGATGATAATGGCAGTTC 1 10.0 No Hit GAATGAAACAAACATTCATCTCTTCAAGCTTCTATGACTGGGTACTCGATA 1 10.0 No Hit GTCTATCTCTACTTCATCCCTAACAAAGATATAGGCCATCAAAAGAGAACA 1 10.0 No Hit TCCGTAAGGTTACTAAGGAAGAGGAAAGCATAAGAGAGCTTTCTTTTGTCT 1 10.0 No Hit GTCAAATTCAAAAAGATGTAACATCTCTTCTTTGGCTGTCTCTTATACAAT 1 10.0 No Hit TATCAATGACAGACAAGATGTCATTACAAACTTTAGCACTGTCTCTTATAC 1 10.0 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 0.0 36 0.0 0.0 10.0 37 0.0 0.0 10.0 38 0.0 0.0 10.0 39 0.0 0.0 20.0 >>END_MODULE >>Kmer Content pass >>END_MODULE