FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_19.3410000000ae10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_19.3410000000ae10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1782014
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC52610.2952277591534073No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6950.038.20010445
ATGCCGT7800.033.7488244
GCCCACG8300.031.98683418
CTACCGC8250.031.90797628
CCCACGA8400.031.60604119
CCGCTGA8450.030.88649631
CCACGAG8650.030.69257220
CGAGCCC8700.030.51617615
ACTACCG8650.030.43246527
CACGAGA8800.030.16940121
GTATGCC8900.030.08322142
TCCTAGG453.4927445E-529.9989512
TACCGCT8950.029.91515529
AGCCCAC9000.029.7489617
ACCGCTG8950.029.66376930
CCGAGCC9050.029.58460214
GAGACTA9000.029.24897824
TCGTATG9000.028.99898740
GATCTCG8950.028.90960536
ATCTCGT9050.028.83877237
Produced by FastQC (version 0.11.2)