FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_18.3410000000ae03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_18.3410000000ae03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863304
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC25840.29931518908750565RNA PCR Primer, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3300.042.27147745
GCCCACG4200.034.284718
TATGCCG4100.033.4746243
CGAGCCC4250.033.3519515
ATGCCGT4250.032.82255644
CCCACGA4450.032.35859319
CGAGACC4400.031.70360623
CCACGAG4500.031.4990720
GAGCCCA4650.030.96682516
AGCCCAC4600.030.81430617
ACCTGTG4650.029.99911127
CGTATGC4600.029.83607541
CACGAGA4800.029.53037621
TCGTATG4650.029.51525540
TCCGAGC4900.029.38688313
CCGAGCC4850.029.22593914
ACGAGAC4850.029.22593922
GTATGCC4700.029.20126242
CCTGTGG5000.028.3491628
GGACATC4950.028.18098433
Produced by FastQC (version 0.11.2)