FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_15.3410000000addb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_15.3410000000addb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421559
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA25350.17832534562406485No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC24710.17382324616846714RNA PCR Primer, Index 23 (95% over 21bp)
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT20220.14223820467528958No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA19780.1391430112995662No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3100.036.28941345
AGGTCGG250.002107535335.9990965
CGTATGC3650.032.05399341
CGAGACG3650.032.05399323
GCCCACG3600.031.24921818
CCCACGA3750.030.59923619
GTCGGTC300.00514829429.999257
GGGATCC300.00514829429.999257
AGGGTCG453.4920162E-529.99924910
GAGGGTC453.4920162E-529.9992499
GCGTAAC453.4920162E-529.99924938
CGAGCCC3950.028.48029915
CCACGAG4050.028.33262420
CGGGTGT406.16777E-428.12429616
CCCTCGC406.16777E-428.12429615
CCGAGCC4000.028.12429414
GAGCCCA4000.028.12429416
TCCGAGC4100.027.98710413
ATGCCGT4100.027.98710444
TATGCCG4300.027.20862443
Produced by FastQC (version 0.11.2)