FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_14.3410000000adce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_14.3410000000adce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188614
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC5970.31651945242664914RNA PCR Primer, Index 45 (95% over 21bp)
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA3220.17071903464217927No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT2580.13678730104870263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAC302.1366504E-645.0584031
TGCCGTC700.044.99867645
ATGCCGT750.041.99876444
GCCCACG800.039.3738418
CGTATGC800.039.3738441
CCCACGA800.039.3738419
CGTTACT356.221815E-638.5702932
CACGAGA850.037.0577321
TATGCCG850.037.0577343
GAGACGT850.037.0577324
CGAGACG850.037.0577323
CCACGAG850.037.0577320
TAGACGC250.002102959235.9989414
AGCCCAC900.034.9989717
GTATGCC900.034.9989742
GAGCCCA900.034.9989716
CGAGCCC900.034.9989715
CTTATAG401.5539992E-533.7490043
ACGTTCA950.033.15691827
GACGTTC950.033.15691826
Produced by FastQC (version 0.11.2)