FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_13.3410000000ada5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_13.3410000000ada5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575216
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC17110.2974534783455259No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7410.12882117326360878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG2300.036.19460318
CGTATGC2350.033.50966641
GTATGCC2450.033.0602742
CTCGTAT2550.031.76378839
CGAGCCC2800.031.33837915
TCGTATG2650.030.56515340
CGGGGCC300.005145875729.9991317
CCCACGA2800.029.73128119
GACCCTA2900.029.48190526
ATGCCGT2750.029.45369344
CCGAGCC3000.029.24915114
CCCTAGA2950.028.9822128
TATCTCG2800.028.92773436
CCACGAG2900.028.70606420
CTAGAAT3100.028.3056330
ACCCTAG3050.028.03197327
TGCCGTC2900.027.93022545
TAGAATA3000.027.74919531
CACGAGA3000.027.74919521
TATGCCG2950.027.45683143
Produced by FastQC (version 0.11.2)