FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_11.3410000000ad61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_11.3410000000ad61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172267
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC23651.3728688605478705No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5250.30475947221464356No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT3480.20201199301084943No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3270.1898216141222637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.13815762740397175No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1740.10100599650542472No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAA206.948128E-445.089591
CTCGTAT3150.039.28394339
TGCCGTC3150.039.28394345
CGTATGC3150.038.56968741
CCCACGA3300.038.18009619
GAGACTA3250.038.07520724
TCGTATG3250.038.07520740
TATGCCG3200.037.96703743
CTCCGAG3300.037.49830612
AGCCCAC3300.037.49830617
GCCCACG3400.037.0571518
TCCGAGC3350.036.93863313
CCGAGCC3350.036.93863314
ACTAAGC3350.036.93863327
CGAGACT3350.036.93863323
GTATGCC3300.036.8165242
ATGCCGT3300.036.8165244
AGACTAA3450.036.52009225
CGAGCCC3450.036.52009215
GATCTCG3500.035.99837536
Produced by FastQC (version 0.11.2)