##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_4_10.3410000000ad48.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 50089 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.94407953842161 38.0 38.0 38.0 32.0 38.0 2 36.985545728603086 38.0 38.0 38.0 32.0 38.0 3 36.9259518057857 38.0 38.0 38.0 32.0 38.0 4 37.029447583301724 38.0 38.0 38.0 32.0 38.0 5 37.03002655273613 38.0 38.0 38.0 32.0 38.0 6 39.00840503903053 40.0 40.0 40.0 38.0 40.0 7 38.900217612649485 40.0 38.0 40.0 38.0 40.0 8 38.90548823094891 40.0 38.0 40.0 38.0 40.0 9 38.918724670087244 40.0 38.0 40.0 38.0 40.0 10 38.846712851125 40.0 38.0 40.0 38.0 40.0 11 38.863942182914414 40.0 38.0 40.0 38.0 40.0 12 38.79634251033161 40.0 38.0 40.0 38.0 40.0 13 38.82672842340634 40.0 38.0 40.0 38.0 40.0 14 38.857593483599196 40.0 38.0 40.0 38.0 40.0 15 38.85783305715826 40.0 38.0 40.0 38.0 40.0 16 38.820958693525526 40.0 38.0 40.0 38.0 40.0 17 38.81459002974705 40.0 38.0 40.0 38.0 40.0 18 38.83259797560343 40.0 38.0 40.0 38.0 40.0 19 38.86525983748927 40.0 38.0 40.0 38.0 40.0 20 38.859969254726586 40.0 38.0 40.0 38.0 40.0 21 38.84573459242548 40.0 38.0 40.0 38.0 40.0 22 38.82061929765018 40.0 38.0 40.0 38.0 40.0 23 38.81307273053964 40.0 38.0 40.0 38.0 40.0 24 38.8594701431452 40.0 38.0 40.0 38.0 40.0 25 38.86326339116373 40.0 38.0 40.0 38.0 40.0 26 38.829164087923495 40.0 38.0 40.0 38.0 40.0 27 38.78799736469085 40.0 38.0 40.0 38.0 40.0 28 38.76941044940007 40.0 38.0 40.0 38.0 40.0 29 38.662979895785504 40.0 38.0 40.0 38.0 40.0 30 38.71927968216575 40.0 38.0 40.0 38.0 40.0 31 38.69783784862944 40.0 38.0 40.0 38.0 40.0 32 38.65513386172613 40.0 38.0 40.0 38.0 40.0 33 38.614486214538125 40.0 38.0 40.0 38.0 40.0 34 38.53033600191659 40.0 38.0 40.0 38.0 40.0 35 38.5037832657869 40.0 38.0 40.0 38.0 40.0 36 38.40270318832478 40.0 38.0 40.0 38.0 40.0 37 38.34618379284873 40.0 38.0 40.0 38.0 40.0 38 38.28698915929645 40.0 38.0 40.0 38.0 40.0 39 38.187426381041746 40.0 38.0 40.0 38.0 40.0 40 38.11631296292599 40.0 38.0 40.0 38.0 40.0 41 38.04182555052007 40.0 38.0 40.0 38.0 40.0 42 37.9492303699415 40.0 38.0 40.0 38.0 40.0 43 37.812873085907086 40.0 38.0 40.0 32.0 40.0 44 37.669687955439315 40.0 38.0 40.0 32.0 40.0 45 37.52642296711853 40.0 38.0 40.0 32.0 40.0 46 37.32490167501847 40.0 38.0 40.0 32.0 40.0 47 37.10159915350676 40.0 38.0 40.0 32.0 40.0 48 36.94244245243467 40.0 38.0 40.0 27.0 40.0 49 36.843897861805985 40.0 38.0 40.0 27.0 40.0 50 36.72045758549781 40.0 38.0 40.0 27.0 40.0 51 36.31761464593024 40.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.52321535501477 2201 2 -0.5338147278425467 2201 3 0.44400543806972337 2201 4 -0.3996448696358925 2201 5 0.24691145742574605 2201 6 0.36968036440717356 2201 7 0.45509286019044026 2201 8 0.11638505623671591 2201 9 0.37849515704753145 2201 10 -0.11321539089193067 2201 11 0.12415201062545833 2201 12 -0.480886174596975 2201 13 -0.35996605687557093 2201 14 -0.8881202587622496 2201 15 -0.33288722751561295 2201 16 -0.2293778424177617 2201 17 0.15005166944353476 2201 18 -0.2711652917079945 2201 19 0.0454797052229452 2201 20 -0.4183874985414775 2201 21 0.09030903917957289 2201 22 0.10038907583791001 2201 23 0.3424303679170677 2201 24 0.2284769453920532 2201 25 -0.15112209953802136 2201 26 0.37877149372739893 2201 27 0.5230841659797463 2201 28 0.4408635750612788 2201 29 0.6689082011279197 2201 30 0.4318374228825945 2201 31 0.16270900410208355 2201 32 0.11290750988316489 2201 33 -0.04780281168959277 2201 34 -0.02993958343868286 2201 35 -0.3467845983457849 2201 36 0.29738997848602367 2201 37 0.5092671793124666 2201 38 -0.062159725347825656 2201 39 0.7351636104703161 2201 40 0.14386316265467514 2201 41 0.3515631016236114 2201 42 -0.21628233801839514 2201 43 0.7246745068227227 2201 44 0.07541761622610466 2201 45 0.5073686090020644 2201 46 0.010134877168631817 2201 47 0.9966212770626655 2201 48 0.5271409221295116 2201 49 1.23012235806722 2201 50 1.2607162170636386 2201 51 0.928026834796519 1101 1 -0.8951960313432679 1101 2 0.13354206994902995 1101 3 -0.13087378898341484 1101 4 -0.21261933478427153 1101 5 0.5091612848929685 1101 6 0.028065457574875552 1101 7 0.18870224597373664 1101 8 0.06462522186818376 1101 9 0.013415791968164115 1101 10 0.29741262843174354 1101 11 0.5126958339518879 1101 12 -0.36494414561146726 1101 13 -0.47659821698599103 1101 14 0.20918822985058227 1101 15 0.3972715026431217 1101 16 0.29236128801702677 1101 17 0.09967209732482729 1101 18 0.2795593459731691 1101 19 0.19040724145482812 1101 20 0.22136405425355576 1101 21 0.07581628555638531 1101 22 0.016193078598433885 1101 23 0.6302150470060965 1101 24 0.37685513724850495 1101 25 0.17254939804652025 1101 26 0.5292200789585877 1101 27 0.02066870704255308 1101 28 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0.49147243518792294 2202 14 0.5955106936186993 2202 15 0.423313169309786 2202 16 0.166455490915574 2202 17 0.4144365900784521 2202 18 0.3121680416253412 2202 19 0.4829797052229452 2202 20 0.386672025268048 2202 21 0.3871840391795729 2202 22 0.8196351075839416 2202 23 0.10507917744087791 2202 24 0.7889928184079267 2202 25 0.24297512268420007 2202 26 0.5308052238861265 2202 27 0.10988972153530341 2202 28 -0.06459277414506914 2202 29 0.6773407408104575 2202 30 0.7249921847873537 2202 31 0.1632050358481152 2202 32 -0.2762293948787402 2202 33 -0.23009447835625707 2202 34 0.007262797513696739 2202 35 -0.24236991580610123 2202 36 0.06623918483523283 2202 37 -0.2402367889415018 2202 38 -0.130364090427193 2202 39 -0.3119594054027033 2202 40 0.3405397499562639 2202 41 0.43737659368710524 2202 42 0.23932282071176303 2202 43 -0.003004064605846679 2202 44 0.24952475908325056 2202 45 0.6613864661449185 2202 46 0.7068114644702206 2202 47 0.9792601659515512 2202 48 0.7840853665739544 2202 49 1.0088921993370619 2202 50 0.7292182011906192 2202 51 0.9540685014631833 1102 1 0.7714706353233964 1102 2 0.0900638090794672 1102 3 0.5647783849296317 1102 4 0.3236125492736974 1102 5 0.668581574748039 1102 6 0.37589154453139173 1102 7 0.34812253582880714 1102 8 0.4269440624478946 1102 9 -0.03006246890139863 1102 10 0.4278474110404389 1102 11 0.46921757308232515 1102 12 0.4321573036638924 1102 13 0.2625322177966183 1102 14 0.5135360559375428 1102 15 0.17988019829529378 1102 16 0.49525983874166 1102 17 -0.030762685283868052 1102 18 0.16361731698766135 1102 19 0.8135956472519297 1102 20 0.3662915904854387 1102 21 0.4381351261360962 1102 22 0.5669177162795904 1102 23 0.5867367861365267 1102 24 0.6377247024658956 1102 25 0.5493609922494187 1102 26 0.07994471663975133 1102 27 0.10762522878167857 1102 28 0.6858601244056572 1102 29 0.9187356683466916 1102 30 0.5560610253670646 1102 31 0.7886579343988416 1102 32 0.5559716920777831 1102 33 0.4014725506292507 1102 34 -0.02579879668920171 1102 35 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4.0 21 5.0 22 9.0 23 24.0 24 38.0 25 84.0 26 122.0 27 183.0 28 245.0 29 365.0 30 498.0 31 646.0 32 820.0 33 1024.0 34 1299.0 35 1719.0 36 2205.0 37 3314.0 38 6059.0 39 31408.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.898122413069125 18.62590230149367 13.902941352902362 22.57303393253484 2 15.819840683583223 23.755714827606862 40.19645031843319 20.22799417037673 3 17.81429056279822 29.126155443310907 32.06492443450658 20.994629559384297 4 11.908802331849309 19.311625306953623 39.1183693026413 29.661203058555767 5 13.16855996326539 37.762782247599276 33.03320090239374 16.0354568867416 6 26.43294935015672 41.24658108566751 16.089360937531193 16.231108626644573 7 24.544311126195375 35.20533450458184 21.996845614805647 18.25350875441714 8 25.069376509812535 33.596198766196174 20.168100780610512 21.16632394338078 9 26.971989858052662 15.588252909820518 20.503503763301325 36.93625346882549 10 17.74241849507876 28.082014015053204 29.455569087025097 24.71999840284294 11 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24 21.86707660364551 26.752380762243206 31.000818542993468 20.379724091117808 25 20.585358062648485 27.820479546407395 31.454011858891175 20.140150532052946 26 21.08446964403362 27.03587614046996 31.17251292698996 20.70714128850646 27 20.44361037353511 28.42739922937172 30.976861187086985 20.15212921000619 28 20.136157639401866 27.093773083910637 31.198466729221984 21.571602547465513 29 20.7929884805047 27.946255664916446 31.168520034338876 20.092235820239974 30 19.800754656711053 27.914312523707803 30.689372916209145 21.595559903371996 31 20.353770288885784 26.890135558705502 31.95911277925293 20.79698137315578 32 20.771027570923756 27.143684242049154 31.44402962726347 20.64125855976362 33 20.577372277346324 27.6088562359001 31.477969214797657 20.33580227195592 34 20.29986623809619 28.748827087783745 30.739284074347662 20.212022599772407 35 19.85266226117511 27.842440455988342 30.715326718441176 21.589570564395377 36 20.016370859869433 27.768571941943343 30.683383577232526 21.5316736209547 37 19.800754656711053 28.90454990117591 30.67140489927928 20.623290542833754 38 19.34955778713889 27.377268462137394 31.791411287907522 21.481762462816185 39 19.421429854858353 27.443151190880233 30.875042424484416 22.260376529776995 40 19.621074487412407 26.746391423266587 32.544071552636304 21.088462536684702 41 19.619078041086865 26.466888937690907 31.382139791171713 22.53189323005051 42 19.93252011419673 26.999940106610232 31.436043841961308 21.631495937231726 43 18.848449759428217 27.01391523088902 32.29052286929266 21.847112140390106 44 18.616861985665516 27.858412026592667 31.342210864660903 22.182515123080915 45 17.88416618419214 27.598874004272396 32.13280360957496 22.384156201960508 46 19.549202419692946 27.211563417117528 30.88502465611212 22.354209507077403 47 18.205594042604165 27.217552756094154 30.723312503743337 23.853540697558344 48 18.1137575116293 27.303399948092395 30.601529277885366 23.981313262392938 49 18.982211663239433 27.40322226436942 30.282097865798878 23.332468206592267 50 17.428976421968894 27.395236479067258 31.278324582243606 23.89746251672024 51 17.880173291541055 27.65677094771307 29.790972069715906 24.672083691029968 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 15.0 1 12.0 2 9.0 3 33.0 4 57.0 5 64.5 6 72.0 7 70.0 8 68.0 9 88.0 10 108.0 11 182.0 12 256.0 13 433.5 14 611.0 15 721.5 16 832.0 17 867.5 18 903.0 19 850.5 20 798.0 21 780.0 22 762.0 23 808.5 24 855.0 25 937.0 26 1126.0 27 1233.0 28 1467.5 29 1702.0 30 1794.5 31 1887.0 32 2024.5 33 2162.0 34 2196.5 35 2231.0 36 2230.0 37 2229.0 38 2234.0 39 2239.0 40 2373.5 41 2508.0 42 2493.5 43 2479.0 44 2581.0 45 2683.0 46 2763.0 47 2843.0 48 2819.5 49 2796.0 50 2741.0 51 2686.0 52 2655.0 53 2624.0 54 2737.0 55 2850.0 56 2359.0 57 1868.0 58 1712.0 59 1556.0 60 1346.5 61 1137.0 62 1070.0 63 1003.0 64 871.5 65 740.0 66 660.0 67 580.0 68 527.5 69 475.0 70 433.5 71 392.0 72 341.0 73 290.0 74 252.5 75 174.0 76 133.0 77 101.0 78 69.0 79 56.5 80 44.0 81 38.5 82 33.0 83 30.5 84 28.0 85 17.5 86 7.0 87 4.0 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15572281339216196 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 50089.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.48884436468666 #Duplication Level Percentage of deduplicated Percentage of total 1 88.98393068193138 72.5119767829797 2 7.857408177828392 12.80582224225748 3 1.8297016465784104 4.473008181355169 4 0.5700110208176802 1.8579815744627242 5 0.20757511721149385 0.8457528210214504 6 0.16561753226930595 0.8097588786694164 7 0.08326473052982498 0.4749602667048737 8 0.03763735253560084 0.2453619490457937 9 0.03590833894957132 0.263351613965043 >10 0.21174932102558808 2.9835343218860646 >50 0.007369838444420341 0.3563314233786299 >100 0.007369681408751682 1.3312121206261172 >500 0.002456560469583893 1.0409478236474896 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC 520 1.0381520892810798 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 299 0.5969374513366208 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 185 0.3693425702249995 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 181 0.36135678492283735 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 69 0.1377547964622971 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 56 0.11180099423027012 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53 0.10581165525364851 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.001996446325540538 2 0.0 0.0 0.001996446325540538 3 0.0 0.0 0.001996446325540538 4 0.0 0.0 0.001996446325540538 5 0.0 0.0 0.001996446325540538 6 0.0 0.0 0.001996446325540538 7 0.0 0.0 0.001996446325540538 8 0.0 0.0 0.001996446325540538 9 0.0 0.0 0.001996446325540538 10 0.0 0.0 0.001996446325540538 11 0.0 0.0 0.001996446325540538 12 0.0 0.0 0.001996446325540538 13 0.0 0.0 0.001996446325540538 14 0.0 0.0 0.001996446325540538 15 0.0 0.0 0.001996446325540538 16 0.0 0.0 0.001996446325540538 17 0.0 0.0 0.001996446325540538 18 0.0 0.0 0.001996446325540538 19 0.0 0.0 0.001996446325540538 20 0.0 0.0 0.001996446325540538 21 0.0 0.0 0.005989338976621614 22 0.0 0.0 0.007985785302162152 23 0.0 0.0 0.007985785302162152 24 0.0 0.0 0.00998223162770269 25 0.0 0.0 0.015971570604324303 26 0.0 0.0 0.021960909580945917 27 0.0 0.0 0.033939587534189145 28 0.0 0.0 0.053904050789594524 29 0.0 0.0 0.15971570604324303 30 0.0 0.0 0.32941364371418874 31 0.0 0.0 0.6708059653816207 32 0.0 0.0 1.0641058915131067 33 0.0 0.0 1.4633951566212142 34 0.0 0.0 1.8447164047994569 35 0.0 0.0 2.341831539859051 36 0.0 0.0 2.864900477150672 37 0.0 0.0 3.5616602447643193 38 0.0 0.0 4.5479047295813455 39 0.0 0.0 5.67988979616283 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGCCGT 70 0.0 38.569714 44 TGCCGTC 75 1.8189894E-12 35.9984 45 CGTATGC 80 1.8189894E-12 33.748505 41 CTCGTAT 80 1.8189894E-12 33.748505 39 TATGCCG 80 1.8189894E-12 33.748505 43 TCTCGTA 80 1.8189894E-12 33.748505 38 TCGTATG 80 1.8189894E-12 33.748505 40 CTGGACC 80 1.8189894E-12 33.748505 30 GCCCACG 85 5.456968E-12 31.763294 18 CCCACGA 85 5.456968E-12 31.763294 19 GTATGCC 85 5.456968E-12 31.763294 42 CCGAGCC 85 5.456968E-12 31.763294 14 GAGCCCA 85 5.456968E-12 31.763294 16 CGAGCCC 85 5.456968E-12 31.763294 15 CGAGACT 85 5.456968E-12 31.763294 23 ATCTCGT 90 1.0913936E-11 29.998667 37 ATCTCCG 90 1.0913936E-11 29.998667 10 CTCCGAG 90 1.0913936E-11 29.998667 12 TGGACCA 90 1.0913936E-11 29.998667 31 AGCCCAC 90 1.0913936E-11 29.998667 17 >>END_MODULE