FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_10.3410000000ad48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_10.3410000000ad48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50089
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC5201.0381520892810798No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.5969374513366208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.3693425702249995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.36135678492283735No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA690.1377547964622971No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.11180099423027012No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.10581165525364851No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT700.038.56971444
TGCCGTC751.8189894E-1235.998445
CGTATGC801.8189894E-1233.74850541
CTCGTAT801.8189894E-1233.74850539
TATGCCG801.8189894E-1233.74850543
TCTCGTA801.8189894E-1233.74850538
TCGTATG801.8189894E-1233.74850540
CTGGACC801.8189894E-1233.74850530
GCCCACG855.456968E-1231.76329418
CCCACGA855.456968E-1231.76329419
GTATGCC855.456968E-1231.76329442
CCGAGCC855.456968E-1231.76329414
GAGCCCA855.456968E-1231.76329416
CGAGCCC855.456968E-1231.76329415
CGAGACT855.456968E-1231.76329423
ATCTCGT901.0913936E-1129.99866737
ATCTCCG901.0913936E-1129.99866710
CTCCGAG901.0913936E-1129.99866712
TGGACCA901.0913936E-1129.99866731
AGCCCAC901.0913936E-1129.99866717