FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_4_1.3410000000ac12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_4_1.3410000000ac12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348666
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC6080.17437891850653633No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCGG300.005086214330.0674631
ACAGGTA300.00514368129.9984737
CCCGGAC300.00514368129.998473
CGGACCC300.00514368129.998475
GTATCAA4300.027.7949221
CCTAGAG450.001225205124.9987269
GGTATCA1201.6370905E-1124.4298131
CGGGGCA500.002262140422.49885417
CCCACGA754.592635E-520.9989319
ATTAGGT654.2315322E-420.7681713
CGTATGC654.2315322E-420.76817141
CGAGCCC654.2315322E-420.76817115
ATACCCG550.003932284720.4535035
CCCTTAA807.4145384E-519.7317731
GCCCACG706.972516E-419.2847318
TAGAACA706.972516E-419.284734
TATGCCG706.972516E-419.2847343
GTTCTAC600.006417819818.7921641
AATAGAC600.006503527518.7490443
CTAGGAG600.006503527518.7490443
Produced by FastQC (version 0.11.2)