FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_94.3410000000abea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_94.3410000000abea.fastq.gz
File typeConventional base calls
EncodingIllumina 1.3
Total Sequences16
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTATGATGGCGTGAAAAAGATATCTGTCACTTACATTAGCACTCTTATA16.25No Hit
ATATGATTCCATTCCATTCAAGTCCATTACATTTGGTTCAATTCCATTCCA16.25No Hit
CTCCAGATGAATCCGATTCACAAGAAGATTCCTGTTCTCATCCACAATGGT16.25No Hit
CTAGTGAATGCATGACAGTGCATTGTGCTAGGCACTGGGTATGTTCAGAGG16.25No Hit
ATATTCCTCCGCATCATTCATAGCATCCAATTGTATACACTTAGACTGATT16.25No Hit
ACGTTATACGTGCATGTCTTGGGTTTGATGTTTCTGAATTGATATTTTGGG16.25No Hit
TTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCG16.25No Hit
GTCCTAACCTCATCGATCCACTTAGTAGTGTTTAGGAAGGACTGCCTGCTT16.25No Hit
CCTTGATGTGGTCTACAATGCCTCCAACAATGAGCTTGTACGTACTAAGAC16.25No Hit
TTGTTGGTAGTGAGCTGATTCAGAAGTATTTGGGAGATGGTCCTAAGCTTG16.25No Hit
TCCTTCGAGTCATCGTTGTTGAGCAACTCTTGGTCCACCCGTAAAACAGCG16.25No Hit
TGATGATTCCATGCGATTCCATTAGATGATGACCCCTTTCATTTCCATTCA16.25No Hit
CCAATGAAGGGTATGCTTACTGGTCCCGTCACCATTCTCAACTGGTCCTTT16.25No Hit
GTAAAGTCTTGGGTATCTTCTTGTGTTTTGTGATCAACTTTGGAAAAGCAT16.25No Hit
ATGTAATGGACTCCTCTTTGAAGACAAGACACTGCTGGAATAAGAGGAAAA16.25No Hit
GTTTTAAGGCTCTCATATCTACAATGATTTACTACCTACTCCGACTGTCTC16.25No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers