FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_87.3410000000ab70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_87.3410000000ab70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGAGTTAAAGAAGACGAAGTAAGCAAGAAGAGCACAAAGAAGAGCC14.3478260869565215No Hit
GTTAAGAGGAGAGTAACTCCACTTCTCACACATATCTTCCTGTCTCTTATA14.3478260869565215No Hit
AGATTGAATTGCTAAGACCAATTCTTCATAACCCTTTTTTTACGATGATAT14.3478260869565215No Hit
GCCATGAGTCCAGGCATCTCAACTTCGGCGAGCTCGAGTTCGAGACGACCG14.3478260869565215No Hit
AGTCACAACAATACAACAACAGACTAGTTTCTGTAAAGCAACAGGTTTAGT14.3478260869565215No Hit
ACCCTGTTCTCTTGCTTCATCCTTTAGGAGGTTTCACTAAAGCTGATGATG14.3478260869565215No Hit
GCAATATGACAGTAGAGAAGATCTCTCACAAAAGACCCAAAATCGAGTCGT14.3478260869565215No Hit
GAATGGCTGCTTCCAAGCCAACATCCTAGCTGTCAATGCAGTCCAACCGCG14.3478260869565215No Hit
ATGTGACACTCTAAAGGAGAAAGTAAAACAGGTCAGGCACTGTCTCTTATA14.3478260869565215No Hit
TTGTTATGTGTGAATTTGATCCTGTCATGATGATGTTAGCTGGTTATTTTG14.3478260869565215No Hit
CCCCAAAACAATCCTTCCATCTGAATGGATCTTACAGCTTCCTCTAGCTTC14.3478260869565215No Hit
CGCCCTAGGTTTTGCAGCGACCGCCGCGCCCTCCTACTCATCGAGGCCCCG14.3478260869565215No Hit
ACATACCGGGGCATCAAAGCATTTGATTAAGAGATTATTTGCTGTCTCTTA14.3478260869565215No Hit
CATCGATATGATGAAACAAAGGCACAAAGTAGAGAGGGAAAACAACATTTG14.3478260869565215No Hit
ACCCTATCTTTGCTGATTTGGGGGTGTTTTAAGAGAAAGTTTGAAGCTTTG14.3478260869565215No Hit
TCTCATGAGGGTATCAACAGTAGGTTGGCTCTTGTTATGAAGAGTGGTAAA14.3478260869565215No Hit
GATTTGGAATGAACATTTTGAGTTCATTGTGGAAGATGTCTCAACTCAACA14.3478260869565215No Hit
GATTACATCTCAGGTTCCATTCTCTTCGAGGAGACTCTTTTACCAGTCCAC14.3478260869565215No Hit
CGATGAATTGTCTGATTTGTCCTCCAATGCTAGATTATGGGGCTCTAGATG14.3478260869565215No Hit
ATAATAGTCAACTGGTACCATAACAAATCAAATTTGCACCATGTCTGGGAC14.3478260869565215No Hit
GTCAAAGACCCTGTAATATCAACTCAACAATGCATGTACTACAACTACAGC14.3478260869565215No Hit
GTCAACGAGCTTAACCTTAAGGACACAGAGCCTGTCTCTTATACACATCTC14.3478260869565215No Hit
GATCTAAGTTGTTAAAGTAGTTCTCAGCTGCTTCTAAACCTTTAGTCCTGT14.3478260869565215No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers