FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_86.3410000000ab63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_86.3410000000ab63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTTGGTCCCCTTCTTTTCTTCTCCACCTTCTTGCTTTATGTCTGTCTCT12.7027027027027026No Hit
TTCACAAATAGAGTTCATATGCCAGATCTGGTATTGCCGTAAGTTCTGCTG12.7027027027027026No Hit
ACCGAAGCCAAAAAAATGCCGGCGGCGAAGAGGACTCCCAAGGTTAACCGT12.7027027027027026No Hit
TGTTCAGACTGCTCTCTCCGCCGATTCATCTGGCGGCGTTCAGTCTTCTTT12.7027027027027026No Hit
ATTCAGGATCGTGAGGCAGCTCGGCGAAGGAGGTTTCGCATTCGTCTTTTT12.7027027027027026No Hit
TCAAAACACAGACAATCAAAAGAAACTGAACAATGTATTTCATCGTAAGAA12.7027027027027026No Hit
ATCAAGTAGTGCCCACATTCGGGTATAAACATAACTTTATTTCCTGTCTCT12.7027027027027026No Hit
ATGTATGTTTGATGACTAATTGTTGAGTATGGAGAGAGTGATTTTGCTTTT12.7027027027027026No Hit
AGTTAAGTCCTTCACAGAGAATAATCACAGCAGAAACATCAAAGATGAAGA12.7027027027027026No Hit
GGAGGAATCTCATCACATCGGACTGTTTCTTCCTCCATAGCTCAGATACAT12.7027027027027026No Hit
CAACAAAGTTCATTTTGTCAGCATCTCTTATGACTGGAACTACAAGACCCT12.7027027027027026No Hit
GTATAGCTACGGATTAGAATTGTGTAATTCTCTCTAAAATCTGTTCGTTGA12.7027027027027026No Hit
ATCTTGGCCGTTTTATTCTAACGTTATTGAGTATCCTCGTCGTCACTTTTT12.7027027027027026No Hit
TCTCAGTACTGTCCACAATCTTCTTAGGACGACAAAAAGGAAGAGAGTAAT12.7027027027027026No Hit
CTCGTTGGGGGTGATGGCGTGAAGGGCTGCCGGCGCTATGCCGGTCGGCAC12.7027027027027026No Hit
GTTAAGAAGAACCTGAAATGAAACCTTGGTTTTAGAGTTCAGACTCGATAA12.7027027027027026No Hit
GAATACTAAGAAGAATGTTTCTTAAAAATTGGATTTTGTGTTGGGTTTTTC12.7027027027027026No Hit
AACATCAACAGTTCCTTTAGGAGCAACTCCAACCGCTGACAAAATCTGAAC12.7027027027027026No Hit
CCCCCAATGCCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGC12.7027027027027026No Hit
ATTCAAGATCATACGATTCTCTTAAACAAGGGAGCATAAGATTCCGGAAAA12.7027027027027026No Hit
CAGCAAAGCCGGTCTCCAATTCCCTGTGGGTCGTATCGCTCGTTTCTTGAA12.7027027027027026No Hit
GTTTGAGAAACATTACCATACTCTTTATTAGTCCATCCCCCAACCATTCAT12.7027027027027026No Hit
AGTTGTGTCTGTCATCAAATGCTTTGGCTTTCTCCATGGCGTCTTTTCCAA12.7027027027027026No Hit
CTCTCTTGGAGCGAATTGGATGATCGGGCCGAGGGCAGCGTAGCGCCTCTT12.7027027027027026No Hit
GAGACAGATACTCTAGAGACCTCAGAAAAAAAGTAATGAAATCTAGAAAAC12.7027027027027026No Hit
GATCCTAGTAAACCTCAGCCTAAAAAATGGCTCTGCTGCATGCAAGCTCCA12.7027027027027026No Hit
GTTTTCCATTCGCCATCACATTTTTGCTTATGCTTAGGTCCCGACTTCCCC12.7027027027027026No Hit
TATACATACATCCTCTTCCTCAGGTTGTTGTAATAAGTTCATTGCAGAAAT12.7027027027027026No Hit
ATAATACCCTTTCTCTGCCAAAGCCTTACTCATCACCTTCACAATGATCCC12.7027027027027026No Hit
AGAACGAGGTGTTCTTTCTTGTGCATAGCGAGTCCAGTCATGACGTCCATC12.7027027027027026No Hit
AGTTTTACCCTACTGATGGAGGGTTATCGCAATAGTAATTCAACTTAGTAC12.7027027027027026No Hit
AAGGTGAAAAGAACCCTATGACGGGAGTGCAATAGAGAACCTGAGATCCGA12.7027027027027026No Hit
ACGGATGGTCGGGCGACAACGTGGGCGCCACACCCTCCACCGCACCCGCGC12.7027027027027026No Hit
GGCATTCGGAGTTTGGCTGGTGTTGGTAAGCGGATAAGCCCCCTTGACCAT12.7027027027027026No Hit
AACTAAGAACGGCCATGCACCACCACCCATAGAATCAAGAAAGAGCTCTCA12.7027027027027026No Hit
CATCTTTCTCGTTTCTACTGGAACAATACTGCAACAAAGCATTCGTGTAAA12.7027027027027026No Hit
TGGTTGATATTTTTCATGGTATTGATAAAGCTGTTGCCGATACTTGGAACA12.7027027027027026No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)