##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_3_85.3410000000ab4a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.19047619047619 0.0 0.0 0.0 0.0 0.0 2 37.476190476190474 0.0 0.0 0.0 0.0 0.0 3 37.42857142857143 0.0 0.0 0.0 0.0 0.0 4 37.714285714285715 0.0 0.0 0.0 0.0 0.0 5 37.714285714285715 0.0 0.0 0.0 0.0 0.0 6 39.80952380952381 0.0 0.0 0.0 0.0 0.0 7 38.42857142857143 0.0 0.0 0.0 0.0 0.0 8 39.23809523809524 0.0 0.0 0.0 0.0 0.0 9 39.42857142857143 0.0 0.0 0.0 0.0 0.0 10 39.61904761904762 0.0 0.0 0.0 0.0 0.0 11 39.42857142857143 0.0 0.0 0.0 0.0 0.0 12 39.333333333333336 0.0 0.0 0.0 0.0 0.0 13 39.61904761904762 0.0 0.0 0.0 0.0 0.0 14 39.80952380952381 0.0 0.0 0.0 0.0 0.0 15 39.61904761904762 0.0 0.0 0.0 0.0 0.0 16 39.80952380952381 0.0 0.0 0.0 0.0 0.0 17 39.80952380952381 0.0 0.0 0.0 0.0 0.0 18 39.80952380952381 0.0 0.0 0.0 0.0 0.0 19 39.714285714285715 0.0 0.0 0.0 0.0 0.0 20 39.80952380952381 0.0 0.0 0.0 0.0 0.0 21 39.61904761904762 0.0 0.0 0.0 0.0 0.0 22 39.80952380952381 0.0 0.0 0.0 0.0 0.0 23 39.42857142857143 0.0 0.0 0.0 0.0 0.0 24 39.714285714285715 0.0 0.0 0.0 0.0 0.0 25 39.714285714285715 0.0 0.0 0.0 0.0 0.0 26 39.714285714285715 0.0 0.0 0.0 0.0 0.0 27 39.61904761904762 0.0 0.0 0.0 0.0 0.0 28 39.714285714285715 0.0 0.0 0.0 0.0 0.0 29 39.80952380952381 0.0 0.0 0.0 0.0 0.0 30 39.714285714285715 0.0 0.0 0.0 0.0 0.0 31 39.904761904761905 0.0 0.0 0.0 0.0 0.0 32 39.80952380952381 0.0 0.0 0.0 0.0 0.0 33 39.714285714285715 0.0 0.0 0.0 0.0 0.0 34 39.714285714285715 0.0 0.0 0.0 0.0 0.0 35 38.523809523809526 0.0 0.0 0.0 0.0 0.0 36 39.0 0.0 0.0 0.0 0.0 0.0 37 39.0 0.0 0.0 0.0 0.0 0.0 38 39.523809523809526 0.0 0.0 0.0 0.0 0.0 39 38.04761904761905 0.0 0.0 0.0 0.0 0.0 40 39.0 0.0 0.0 0.0 0.0 0.0 41 39.61904761904762 0.0 0.0 0.0 0.0 0.0 42 39.142857142857146 0.0 0.0 0.0 0.0 0.0 43 39.142857142857146 0.0 0.0 0.0 0.0 0.0 44 38.04761904761905 0.0 0.0 0.0 0.0 0.0 45 38.04761904761905 0.0 0.0 0.0 0.0 0.0 46 39.23809523809524 0.0 0.0 0.0 0.0 0.0 47 39.333333333333336 0.0 0.0 0.0 0.0 0.0 48 39.333333333333336 0.0 0.0 0.0 0.0 0.0 49 39.61904761904762 0.0 0.0 0.0 0.0 0.0 50 39.23809523809524 0.0 0.0 0.0 0.0 0.0 51 39.04761904761905 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2215 1 0.0 2215 2 0.0 2215 3 0.0 2215 4 0.0 2215 5 3.0 2215 6 0.0 2215 7 -1.0 2215 8 -1.0 2215 9 0.0 2215 10 -1.0 2215 11 -1.0 2215 12 0.0 2215 13 0.0 2215 14 0.0 2215 15 0.0 2215 16 0.0 2215 17 0.0 2215 18 -1.0 2215 19 0.0 2215 20 0.0 2215 21 -1.0 2215 22 -1.0 2215 23 0.0 2215 24 -1.0 2215 25 0.0 2215 26 0.0 2215 27 0.0 2215 28 0.0 2215 29 0.0 2215 30 -1.0 2215 31 0.0 2215 32 1.0 2215 33 0.0 2215 34 0.0 2215 35 0.0 2215 36 0.0 2215 37 0.0 2215 38 0.0 2215 39 0.0 2215 40 0.0 2215 41 0.0 2215 42 0.0 2215 43 0.0 2215 44 0.0 2215 45 0.0 2215 46 0.0 2215 47 0.0 2215 48 0.0 2215 49 0.0 2215 50 0.0 2215 51 0.0 1214 1 0.0 1214 2 0.0 1214 3 0.0 1214 4 0.0 1214 5 -3.0 1214 6 0.0 1214 7 1.0 1214 8 1.0 1214 9 0.0 1214 10 1.0 1214 11 1.0 1214 12 0.0 1214 13 0.0 1214 14 0.0 1214 15 0.0 1214 16 0.0 1214 17 0.0 1214 18 1.0 1214 19 0.0 1214 20 0.0 1214 21 1.0 1214 22 1.0 1214 23 0.0 1214 24 1.0 1214 25 0.0 1214 26 0.0 1214 27 0.0 1214 28 0.0 1214 29 0.0 1214 30 1.0 1214 31 0.0 1214 32 -1.0 1214 33 0.0 1214 34 0.0 1214 35 0.0 1214 36 0.0 1214 37 0.0 1214 38 0.0 1214 39 0.0 1214 40 0.0 1214 41 0.0 1214 42 0.0 1214 43 0.0 1214 44 0.0 1214 45 0.0 1214 46 0.0 1214 47 0.0 1214 48 0.0 1214 49 0.0 1214 50 0.0 1214 51 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 36 1.0 37 2.0 38 2.0 39 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 57.14285714285714 14.285714285714285 9.523809523809524 19.047619047619047 2 14.285714285714285 28.57142857142857 38.095238095238095 19.047619047619047 3 19.047619047619047 23.809523809523807 28.57142857142857 28.57142857142857 4 19.047619047619047 28.57142857142857 42.857142857142854 9.523809523809524 5 33.33333333333333 23.809523809523807 28.57142857142857 14.285714285714285 6 33.33333333333333 23.809523809523807 28.57142857142857 14.285714285714285 7 14.285714285714285 52.38095238095239 28.57142857142857 4.761904761904762 8 23.809523809523807 38.095238095238095 23.809523809523807 14.285714285714285 9 14.285714285714285 23.809523809523807 19.047619047619047 42.857142857142854 10 19.047619047619047 33.33333333333333 23.809523809523807 23.809523809523807 11 28.57142857142857 42.857142857142854 9.523809523809524 19.047619047619047 12 28.57142857142857 19.047619047619047 47.61904761904761 4.761904761904762 13 28.57142857142857 38.095238095238095 9.523809523809524 23.809523809523807 14 14.285714285714285 14.285714285714285 42.857142857142854 28.57142857142857 15 28.57142857142857 19.047619047619047 23.809523809523807 28.57142857142857 16 14.285714285714285 33.33333333333333 23.809523809523807 28.57142857142857 17 14.285714285714285 38.095238095238095 23.809523809523807 23.809523809523807 18 14.285714285714285 23.809523809523807 38.095238095238095 23.809523809523807 19 23.809523809523807 14.285714285714285 47.61904761904761 14.285714285714285 20 33.33333333333333 9.523809523809524 42.857142857142854 14.285714285714285 21 33.33333333333333 33.33333333333333 23.809523809523807 9.523809523809524 22 14.285714285714285 9.523809523809524 33.33333333333333 42.857142857142854 23 23.809523809523807 23.809523809523807 28.57142857142857 23.809523809523807 24 28.57142857142857 9.523809523809524 28.57142857142857 33.33333333333333 25 28.57142857142857 38.095238095238095 19.047619047619047 14.285714285714285 26 28.57142857142857 14.285714285714285 23.809523809523807 33.33333333333333 27 14.285714285714285 33.33333333333333 33.33333333333333 19.047619047619047 28 19.047619047619047 23.809523809523807 38.095238095238095 19.047619047619047 29 23.809523809523807 28.57142857142857 33.33333333333333 14.285714285714285 30 14.285714285714285 42.857142857142854 42.857142857142854 0.0 31 38.095238095238095 9.523809523809524 28.57142857142857 23.809523809523807 32 9.523809523809524 42.857142857142854 33.33333333333333 14.285714285714285 33 28.57142857142857 23.809523809523807 19.047619047619047 28.57142857142857 34 14.285714285714285 33.33333333333333 33.33333333333333 19.047619047619047 35 28.57142857142857 28.57142857142857 14.285714285714285 28.57142857142857 36 4.761904761904762 61.904761904761905 28.57142857142857 4.761904761904762 37 23.809523809523807 38.095238095238095 19.047619047619047 19.047619047619047 38 42.857142857142854 19.047619047619047 23.809523809523807 14.285714285714285 39 14.285714285714285 23.809523809523807 28.57142857142857 33.33333333333333 40 28.57142857142857 9.523809523809524 42.857142857142854 19.047619047619047 41 38.095238095238095 19.047619047619047 23.809523809523807 19.047619047619047 42 14.285714285714285 38.095238095238095 23.809523809523807 23.809523809523807 43 33.33333333333333 14.285714285714285 28.57142857142857 23.809523809523807 44 14.285714285714285 33.33333333333333 38.095238095238095 14.285714285714285 45 23.809523809523807 9.523809523809524 33.33333333333333 33.33333333333333 46 19.047619047619047 28.57142857142857 33.33333333333333 19.047619047619047 47 23.809523809523807 14.285714285714285 38.095238095238095 23.809523809523807 48 14.285714285714285 38.095238095238095 38.095238095238095 9.523809523809524 49 9.523809523809524 19.047619047619047 52.38095238095239 19.047619047619047 50 23.809523809523807 33.33333333333333 38.095238095238095 4.761904761904762 51 28.57142857142857 9.523809523809524 14.285714285714285 47.61904761904761 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.5 29 1.0 30 1.0 31 1.0 32 1.0 33 1.0 34 1.5 35 2.0 36 2.0 37 2.0 38 2.0 39 2.0 40 2.0 41 2.0 42 1.5 43 1.0 44 0.5 45 0.0 46 0.5 47 1.0 48 1.0 49 1.0 50 1.5 51 2.0 52 2.5 53 3.0 54 1.5 55 0.0 56 0.5 57 1.0 58 0.5 59 0.0 60 0.0 61 0.0 62 0.5 63 1.0 64 0.5 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 21.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGCTCGTCTCAGCATCTACGACCCACAAGTCACTGAAGAGCAGATCCAAAG 1 4.761904761904762 No Hit GACAGCTAAGAAATCTAGGGTTAAGTGTTTCATCAAGCTTGTTACTGTCTC 1 4.761904761904762 No Hit GTCATGAACTCTACAAGCCTAGAGAGTGAAAGAGGAGCTTTGTTGACTTTG 1 4.761904761904762 No Hit GTCTTTAGTGAGAGTTTCCTACTACAGAAATTGAATAGCTGTCTCTTATAC 1 4.761904761904762 No Hit TTGCAAAGACCAGCCCATTCGCCGAGTGTTTTGGCACTAGGAACAGTAAGC 1 4.761904761904762 No Hit GACAGCTTCAGTCTGTTTTACATCACCCAACACTTTAGTGAAGTTTGGTTG 1 4.761904761904762 No Hit GCTTAGGACACTCAGCGTAGACACGCTCAAGAGCCTCGACTTGCTCAGCTG 1 4.761904761904762 No Hit ACATGTTTCAAAGCTGAAGCATTCACTATAGTCCCAAATCTATATGCCTTG 1 4.761904761904762 No Hit TGTTGGAGCTGTGACAATTGATGCTGCTGTTGCTGTTGCTGCTGGTGTTGT 1 4.761904761904762 No Hit GTTAAAGAAAATACAAAATGGACCCCAACAGCAAAAACCTGTCTCTTATAC 1 4.761904761904762 No Hit CCAGGTTGTTAGACATCAGTCCCTCGATGTTCATCCTAGTCCGACTTTTTC 1 4.761904761904762 No Hit GTATCCCACCTGGGGAGTACGCACGCAAGTGTGAAACTCAAAGGAATTGAC 1 4.761904761904762 No Hit GTGGGAAAGGATGTGGAGTTGCTGAGACAACCAGGAGGTTGGCTTAGAAGC 1 4.761904761904762 No Hit GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTTC 1 4.761904761904762 No Hit CGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGT 1 4.761904761904762 No Hit AAATATAACAGGCTGAAATATGCTTTATTGGTCAAATGCTGTCTCTTATAC 1 4.761904761904762 No Hit ATTATGTTCAATGTCCCTTTGTGTTCCGTTGAGTTAAACACCAACGTGTAG 1 4.761904761904762 No Hit CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCTATGGCCTCTCCCCGT 1 4.761904761904762 No Hit GTATCAACGCAGAGTACGGGGGAGATATCAAAAAGAAGAGAAGACCAAAAA 1 4.761904761904762 No Hit CATGAGGTATGTCTCCTCTTATGGAATTTTGATCAAGAAGTAAATACTGTA 1 4.761904761904762 No Hit GATCTGAAACGATTAGCCATTTTTGTTGCAGATAGATTTTGATTGGAACCC 1 4.761904761904762 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 0.0 36 0.0 0.0 0.0 37 0.0 0.0 0.0 38 0.0 0.0 0.0 39 0.0 0.0 14.285714285714286 >>END_MODULE >>Kmer Content pass >>END_MODULE