FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_84.3410000000ab3d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_84.3410000000ab3d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTAGGACCTGATTGAGAAGAGAGAAAGCACTTCTGTCTCTTATACACA14.0No Hit
GTCCCATCCAATATTGACACCACAATTGGTGTATCTTTGAGAGCATCGGCG14.0No Hit
CATTAGACAAAAGTAGCACATATAATGTAAAGACGAAACGATGATAAAGAG14.0No Hit
ATCCATAGCAATGGTCCTGACGACATTCTGACCCAAGTGATGAGACACCTC14.0No Hit
TCACATAGAAGAGCTAGCTGAAACACATCAATCACATTCTCTTTGTTGAGC14.0No Hit
CTTCACAGTTACGTTTTCCACGCCTTGCTCCTCTATGGACTTTGCTAAGCT14.0No Hit
AGCGTATACAACAAAGTCTTGAAGCAGAGAAAGAAAAAAGAAAGACAAGAG14.0No Hit
GTCGTGGTAATCCCACCGTTGAGGTTGATATCCACACGTCAAATGGTATTA14.0No Hit
CACGATATGCATGTCTTTTGAGTTCTCCAAAGAAGTCTGCTTTAGTTGCGT14.0No Hit
CTTTTTCTTCTTATTCGTGTGTTCTTCTTGTTGTTGTTGTTGTTGTTCTAT14.0No Hit
AGTCAACCATGCAAGAGTCAGCCTAACAAGTATAACCTCAGCGATAATGGC14.0No Hit
AGCATGTTCAGTGTGCCAATCACATTGGTCTTGATTGTCTTCACAGGGTTG14.0No Hit
AGCAAGAGGCCCACCTTGCCCTCAACCCCTTCGGTCAAATTCCTGCTCTCG14.0No Hit
AAGCAAAGAGAGCTGAGCGGGAACTTGCTAACCGAGGCGGATTTAAAGAGC14.0No Hit
GAAGGAATCAATAGTCTCTGTGAAATGTGAAACCAGAAGACAAATGAAGAA14.0No Hit
GTTTATTAGCCTGCCATGGTTTAAAATGCGTTTACACCATAACATGTCGAT14.0No Hit
CCCCTACTCTAATCAAAAGACTAAAAGGCCCCTACTGAAGGTCGCCAAACT14.0No Hit
TCTTTCGGGTCCGGACAGGCATGCTCACACTCGAACCCTTCTCAGAAGATC14.0No Hit
CTCGAACCCTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCACCCGCGAGGG14.0No Hit
CCAGTATTACAATGTATATGGGAGTGGTCAAGAGTGGACTGACTACCCAGC14.0No Hit
ATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGCTCTCG14.0No Hit
CTTCTCGACACTGAGGCCTTTAAGGATACTATTGCTTTGTTTGTTGATAGA14.0No Hit
GTACAAGCTTAGATCAGAACATGGATGAGTTTATGCAAAACGGTTATTGGA14.0No Hit
ACTTACTGGTGTAGTAGGGTCACTAAGAACAGGGGTCGTCTTGTTTATATT14.0No Hit
GGTTGGTGACATTGGCAGTGCCACTTGGTTCCTTAATGATCTTGCGAGTGA14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers