##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_3_84.3410000000ab3d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.76 0.0 0.0 0.0 0.0 0.0 2 37.52 0.0 0.0 0.0 0.0 0.0 3 37.76 0.0 0.0 0.0 0.0 0.0 4 38.0 0.0 0.0 0.0 0.0 0.0 5 37.76 0.0 0.0 0.0 0.0 0.0 6 39.76 0.0 0.0 0.0 0.0 0.0 7 39.92 0.0 0.0 0.0 0.0 0.0 8 39.6 0.0 0.0 0.0 0.0 0.0 9 39.84 0.0 0.0 0.0 0.0 0.0 10 39.68 0.0 0.0 0.0 0.0 0.0 11 39.68 0.0 0.0 0.0 0.0 0.0 12 39.92 0.0 0.0 0.0 0.0 0.0 13 39.76 0.0 0.0 0.0 0.0 0.0 14 39.84 0.0 0.0 0.0 0.0 0.0 15 39.92 0.0 0.0 0.0 0.0 0.0 16 39.92 0.0 0.0 0.0 0.0 0.0 17 39.92 0.0 0.0 0.0 0.0 0.0 18 39.92 0.0 0.0 0.0 0.0 0.0 19 39.76 0.0 0.0 0.0 0.0 0.0 20 39.84 0.0 0.0 0.0 0.0 0.0 21 39.84 0.0 0.0 0.0 0.0 0.0 22 39.6 0.0 0.0 0.0 0.0 0.0 23 39.32 0.0 0.0 0.0 0.0 0.0 24 39.4 0.0 0.0 0.0 0.0 0.0 25 39.24 0.0 0.0 0.0 0.0 0.0 26 39.08 0.0 0.0 0.0 0.0 0.0 27 39.6 0.0 0.0 0.0 0.0 0.0 28 39.68 0.0 0.0 0.0 0.0 0.0 29 39.6 0.0 0.0 0.0 0.0 0.0 30 39.68 0.0 0.0 0.0 0.0 0.0 31 39.44 0.0 0.0 0.0 0.0 0.0 32 39.76 0.0 0.0 0.0 0.0 0.0 33 39.84 0.0 0.0 0.0 0.0 0.0 34 39.84 0.0 0.0 0.0 0.0 0.0 35 39.92 0.0 0.0 0.0 0.0 0.0 36 39.84 0.0 0.0 0.0 0.0 0.0 37 39.76 0.0 0.0 0.0 0.0 0.0 38 39.84 0.0 0.0 0.0 0.0 0.0 39 39.84 0.0 0.0 0.0 0.0 0.0 40 39.84 0.0 0.0 0.0 0.0 0.0 41 39.84 0.0 0.0 0.0 0.0 0.0 42 39.68 0.0 0.0 0.0 0.0 0.0 43 39.92 0.0 0.0 0.0 0.0 0.0 44 39.92 0.0 0.0 0.0 0.0 0.0 45 39.92 0.0 0.0 0.0 0.0 0.0 46 39.76 0.0 0.0 0.0 0.0 0.0 47 38.84 0.0 0.0 0.0 0.0 0.0 48 39.84 0.0 0.0 0.0 0.0 0.0 49 39.68 0.0 0.0 0.0 0.0 0.0 50 39.68 0.0 0.0 0.0 0.0 0.0 51 39.68 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2207 1 0.0 2207 2 0.0 2207 3 0.0 2207 4 0.0 2207 5 0.0 2207 6 -1.0 2207 7 0.0 2207 8 -4.0 2207 9 -1.0 2207 10 -1.0 2207 11 -1.0 2207 12 -1.0 2207 13 -1.0 2207 14 0.0 2207 15 0.0 2207 16 0.0 2207 17 0.0 2207 18 0.0 2207 19 -1.0 2207 20 0.0 2207 21 1.0 2207 22 0.0 2207 23 0.0 2207 24 -1.0 2207 25 -1.0 2207 26 -1.0 2207 27 -1.0 2207 28 -1.0 2207 29 -1.0 2207 30 0.0 2207 31 0.0 2207 32 0.0 2207 33 -1.0 2207 34 0.0 2207 35 0.0 2207 36 0.0 2207 37 -1.0 2207 38 0.0 2207 39 0.0 2207 40 0.0 2207 41 0.0 2207 42 0.0 2207 43 0.0 2207 44 0.0 2207 45 0.0 2207 46 0.0 2207 47 -1.0 2207 48 0.0 2207 49 -1.0 2207 50 -1.0 2207 51 -1.0 1212 1 0.0 1212 2 0.0 1212 3 0.0 1212 4 0.0 1212 5 0.0 1212 6 1.0 1212 7 0.0 1212 8 4.0 1212 9 1.0 1212 10 1.0 1212 11 1.0 1212 12 1.0 1212 13 1.0 1212 14 0.0 1212 15 0.0 1212 16 0.0 1212 17 0.0 1212 18 0.0 1212 19 1.0 1212 20 0.0 1212 21 -1.0 1212 22 0.0 1212 23 0.0 1212 24 1.0 1212 25 1.0 1212 26 1.0 1212 27 1.0 1212 28 1.0 1212 29 1.0 1212 30 0.0 1212 31 0.0 1212 32 0.0 1212 33 1.0 1212 34 0.0 1212 35 0.0 1212 36 0.0 1212 37 1.0 1212 38 0.0 1212 39 0.0 1212 40 0.0 1212 41 0.0 1212 42 0.0 1212 43 0.0 1212 44 0.0 1212 45 0.0 1212 46 0.0 1212 47 1.0 1212 48 0.0 1212 49 1.0 1212 50 1.0 1212 51 1.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 38 3.0 39 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.0 32.0 8.0 36.0 2 20.0 20.0 40.0 20.0 3 4.0 16.0 40.0 40.0 4 24.0 8.0 24.0 44.0 5 8.0 48.0 36.0 8.0 6 24.0 40.0 20.0 16.0 7 20.0 32.0 28.000000000000004 20.0 8 36.0 16.0 28.000000000000004 20.0 9 20.0 32.0 16.0 32.0 10 4.0 36.0 28.000000000000004 32.0 11 20.0 48.0 12.0 20.0 12 8.0 16.0 44.0 32.0 13 40.0 32.0 16.0 12.0 14 24.0 20.0 32.0 24.0 15 24.0 24.0 36.0 16.0 16 20.0 32.0 20.0 28.000000000000004 17 28.000000000000004 20.0 32.0 20.0 18 24.0 28.000000000000004 16.0 32.0 19 36.0 12.0 36.0 16.0 20 28.000000000000004 28.000000000000004 28.000000000000004 16.0 21 24.0 20.0 20.0 36.0 22 24.0 36.0 28.000000000000004 12.0 23 12.0 48.0 16.0 24.0 24 24.0 36.0 12.0 28.000000000000004 25 8.0 48.0 32.0 12.0 26 12.0 24.0 48.0 16.0 27 48.0 16.0 20.0 16.0 28 20.0 32.0 20.0 28.000000000000004 29 20.0 28.000000000000004 28.000000000000004 24.0 30 24.0 36.0 20.0 20.0 31 4.0 44.0 36.0 16.0 32 16.0 16.0 36.0 32.0 33 32.0 20.0 20.0 28.000000000000004 34 28.000000000000004 36.0 24.0 12.0 35 16.0 32.0 20.0 32.0 36 20.0 28.000000000000004 28.000000000000004 24.0 37 28.000000000000004 20.0 24.0 28.000000000000004 38 20.0 32.0 36.0 12.0 39 32.0 28.000000000000004 16.0 24.0 40 16.0 20.0 40.0 24.0 41 16.0 24.0 40.0 20.0 42 16.0 24.0 40.0 20.0 43 28.000000000000004 20.0 28.000000000000004 24.0 44 20.0 28.000000000000004 40.0 12.0 45 32.0 28.000000000000004 28.000000000000004 12.0 46 24.0 20.0 28.000000000000004 28.000000000000004 47 8.0 52.0 32.0 8.0 48 28.000000000000004 28.000000000000004 16.0 28.000000000000004 49 36.0 32.0 24.0 8.0 50 36.0 20.0 20.0 24.0 51 28.000000000000004 24.0 24.0 24.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 1.0 31 2.0 32 1.0 33 0.0 34 1.0 35 2.0 36 2.0 37 2.0 38 2.0 39 2.0 40 2.5 41 3.0 42 1.5 43 0.0 44 1.5 45 3.0 46 3.5 47 4.0 48 3.0 49 2.0 50 1.5 51 1.0 52 0.5 53 0.0 54 1.0 55 2.0 56 1.0 57 0.0 58 0.5 59 1.0 60 0.5 61 0.0 62 0.0 63 0.0 64 0.5 65 1.0 66 0.5 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 25.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CATCTAGGACCTGATTGAGAAGAGAGAAAGCACTTCTGTCTCTTATACACA 1 4.0 No Hit GTCCCATCCAATATTGACACCACAATTGGTGTATCTTTGAGAGCATCGGCG 1 4.0 No Hit CATTAGACAAAAGTAGCACATATAATGTAAAGACGAAACGATGATAAAGAG 1 4.0 No Hit ATCCATAGCAATGGTCCTGACGACATTCTGACCCAAGTGATGAGACACCTC 1 4.0 No Hit TCACATAGAAGAGCTAGCTGAAACACATCAATCACATTCTCTTTGTTGAGC 1 4.0 No Hit CTTCACAGTTACGTTTTCCACGCCTTGCTCCTCTATGGACTTTGCTAAGCT 1 4.0 No Hit AGCGTATACAACAAAGTCTTGAAGCAGAGAAAGAAAAAAGAAAGACAAGAG 1 4.0 No Hit GTCGTGGTAATCCCACCGTTGAGGTTGATATCCACACGTCAAATGGTATTA 1 4.0 No Hit CACGATATGCATGTCTTTTGAGTTCTCCAAAGAAGTCTGCTTTAGTTGCGT 1 4.0 No Hit CTTTTTCTTCTTATTCGTGTGTTCTTCTTGTTGTTGTTGTTGTTGTTCTAT 1 4.0 No Hit AGTCAACCATGCAAGAGTCAGCCTAACAAGTATAACCTCAGCGATAATGGC 1 4.0 No Hit AGCATGTTCAGTGTGCCAATCACATTGGTCTTGATTGTCTTCACAGGGTTG 1 4.0 No Hit AGCAAGAGGCCCACCTTGCCCTCAACCCCTTCGGTCAAATTCCTGCTCTCG 1 4.0 No Hit AAGCAAAGAGAGCTGAGCGGGAACTTGCTAACCGAGGCGGATTTAAAGAGC 1 4.0 No Hit GAAGGAATCAATAGTCTCTGTGAAATGTGAAACCAGAAGACAAATGAAGAA 1 4.0 No Hit GTTTATTAGCCTGCCATGGTTTAAAATGCGTTTACACCATAACATGTCGAT 1 4.0 No Hit CCCCTACTCTAATCAAAAGACTAAAAGGCCCCTACTGAAGGTCGCCAAACT 1 4.0 No Hit TCTTTCGGGTCCGGACAGGCATGCTCACACTCGAACCCTTCTCAGAAGATC 1 4.0 No Hit CTCGAACCCTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCACCCGCGAGGG 1 4.0 No Hit CCAGTATTACAATGTATATGGGAGTGGTCAAGAGTGGACTGACTACCCAGC 1 4.0 No Hit ATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGCTCTCG 1 4.0 No Hit CTTCTCGACACTGAGGCCTTTAAGGATACTATTGCTTTGTTTGTTGATAGA 1 4.0 No Hit GTACAAGCTTAGATCAGAACATGGATGAGTTTATGCAAAACGGTTATTGGA 1 4.0 No Hit ACTTACTGGTGTAGTAGGGTCACTAAGAACAGGGGTCGTCTTGTTTATATT 1 4.0 No Hit GGTTGGTGACATTGGCAGTGCCACTTGGTTCCTTAATGATCTTGCGAGTGA 1 4.0 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 0.0 36 0.0 0.0 4.0 37 0.0 0.0 4.0 38 0.0 0.0 4.0 39 0.0 0.0 4.0 >>END_MODULE >>Kmer Content pass >>END_MODULE