FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_83.3410000000ab20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_83.3410000000ab20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCATGGGCAGGCAAGAGACAACCTGGCGAACTGAAACATCTTAGTAGCC11.9230769230769231No Hit
CCTCTGGCTTATGCTTAGATTGTGATTTTGGTCCTCTAATTTTAATATCGC11.9230769230769231No Hit
TAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAACTGTCTC11.9230769230769231No Hit
TCCTTGTCCACCTTTTTGCTCGTTTCTTGTAGTAGCTTTTAGCATTCACTC11.9230769230769231No Hit
GCACCAAGAAGAGAATGCTTACCAATAGCCTTCACTCTCTCGATGTTCTTG11.9230769230769231No Hit
GGACAAAACCCAACCGAAGCAGAGCTTCAAGACATGATCAACGAAGTGGAT11.9230769230769231No Hit
GTGATGCTGTCCTTGGAAAAGAAAAACCTGTTGAAGAAACTACTGCTGACT11.9230769230769231No Hit
CTACAAGAATCAGCCTGAATTTGGAATCCTTATCTTTCCTGTTCCTCTCAC11.9230769230769231No Hit
CAACAACAACAGTTCACTTCAGAAAGAGAGAGAGAAGATCTGTCTCTTATA11.9230769230769231No Hit
ACATTGAACGATCTCATAAATTTGACGCACTCAGCATGACCTTTGATTGAA11.9230769230769231No Hit
CATGGACATCACCAGAGAGAGAAGCGTTAGGAGCCACAAAAGCCCCCTTAT11.9230769230769231No Hit
AGCTAAAGATGTTCATGTCATGATTTGGATTTTGGATGCCTTATTTATCCC11.9230769230769231No Hit
ATCCGGAGGACCGAGTGCCGCTCACGCCCGGTCGTACTCATAACCGCATCA11.9230769230769231No Hit
GAAAATCTGTGTTCCATGTGAAGGTGTGGGCTTTATTCAGGAAGTTGTTTC11.9230769230769231No Hit
CCTAATGGCTTAAGCCGCTCTTCCCATTTCCTCGCTGACCCATCATGCAAA11.9230769230769231No Hit
TCGTTAATCAAACCACAAAATCTAACTTCCACACACAAAACACCTGTCTCT11.9230769230769231No Hit
CCATTCCTTTCCATTCCATTCGGTTCCATTCCTTTCCATTTCATTTGGGTT11.9230769230769231No Hit
TGATACCACTGCTTCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCT11.9230769230769231No Hit
TGATGGTCGCACCATCGTTATCCTGACCCGCTCATCAAGCCTAACGACACC11.9230769230769231No Hit
ATCCAGACCATGCATATAGCGGAGAATATCACCTTGATACGCCTCATATCT11.9230769230769231No Hit
CTACCAAATCCTTAAGTACCCACTCACCACTGAATCTGCGATGAAGAAGAT11.9230769230769231No Hit
GTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAGAGTGCTTGCTGTCTC11.9230769230769231No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGC11.9230769230769231No Hit
TTTACAAAGTGCGTGAAGAAACGGTCAAGTTACTAGGAAAGGCCTGTCTCT11.9230769230769231No Hit
TTCTTGTAAGAGATCAGACTAAAAGACTCAGGAAAAAGAAAGAAGCTCAAT11.9230769230769231No Hit
CAACGAATGAGAAGATATCGATTTGGTAAGAAACGAAGAAGAAGATGAAGA11.9230769230769231No Hit
TATCAACGCAGAGTACGGGGAGAACACACACATATACTGAACTCACAGATT11.9230769230769231No Hit
CGCTCATCTTAGTGACAAGATAATCATAGAGCCAGTCTCTTATACACATCT11.9230769230769231No Hit
ATCATAGTATCTTCAGCAGCAGTTTTCCTCTCATCACCAGATTTAAACTCA11.9230769230769231No Hit
TGATTATCAAGGATGAAATTGGAGAACAAGTGAAATATTCTCTGAAAGCAG11.9230769230769231No Hit
TCTTAACCCAGCTCCCGATATGTGGACATGTTTATCTTATGATAATGCATC11.9230769230769231No Hit
ACCTCAAATCCAACAATCTCATACCCCAATGCCTTCTTCTTATCCTGTCTC11.9230769230769231No Hit
CATGTACAGAATGTGCAGGTTTGTTACATAGGTATACATCTGTCTCTTATA11.9230769230769231No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC11.9230769230769231No Hit
ACACTCTTCTTGAAATGTTCACCTTTACATGTAGAACCATCGTAAGCAACG11.9230769230769231No Hit
ATATCAACCTCTGTTGATCAGGTGGTATTCCCTCTTTGTCCTGAATCTGTC11.9230769230769231No Hit
AATCTGGATTATGCAGTCGTAGGCAGTGATGAAGTACAACTTCTAGAGCTG11.9230769230769231No Hit
GAAGAGAAGAAGTGAGGTCGTTCTTCTTAGGTAGAAGAAACTTCTGTCTCT11.9230769230769231No Hit
GTATGAAGGTCAGAATCATGGGTCCCAACTATATCCCTGGTGAGAAGAAGG11.9230769230769231No Hit
GAATCTTCTTAGTCTTAGGGTCAATGGCATTGATACCACGGATACGGATGA11.9230769230769231No Hit
CTTGATTCCCGAAAGAGTGCAAATGGAGGAGCAGTTCAAGTCACTAATTGT11.9230769230769231No Hit
GTCAGATTCCTCATCATCTCGTCTTGTTCCTCAACAGGTAACTGAGCTTCT11.9230769230769231No Hit
TCATTGTTTGTTGTGTACCTTAAAGCCAAAGATCTTAGGAACGTAGTTTTG11.9230769230769231No Hit
GAATTGGTCAGCCTCAATCGTTTTCTTGTAATTTACCTTCATTTTTTTCCC11.9230769230769231No Hit
GAACGATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCT11.9230769230769231No Hit
GGTTCTGCTATGCTTGCCATGGATCTGGGATGGATGGGGCCAAACTATTCT11.9230769230769231No Hit
GGAGAGTAAAGTGCGAGGTACATTGTGGGATTGTATTTCCTTCATCAGGTA11.9230769230769231No Hit
TGGAAATGTAATCTAACTGGAAGAATCTACTTATTAGCAAGCATAGTTCCC11.9230769230769231No Hit
GTGAAGAAGAAGAAGAAGAAGAAGAAGAAAGGTTGCTTGTTTCGCAAATTT11.9230769230769231No Hit
GTTATGATGCTATGTATGAGAGGTGGAAACTATTGAAACAAGCAGCAAAGG11.9230769230769231No Hit
GTTTGAGTCGTTTGGCACCTTAGTTACTATTTCTACTAAGTGGTTTAAAGA11.9230769230769231No Hit
GGCATGTACTGTGCCATTATGGCTCTGATCTCTGAGTCCATGTATCTGTCT11.9230769230769231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCTC50.045.026
CTTCAGC50.045.011
AAACTCA50.045.045
CAGTTTT50.045.020
TAAACTC50.045.044
ATCTTCA50.045.09
CAGCAGT50.045.017
CAGCAGC50.045.014
TTTCCTC50.045.024
CCAGATT50.045.037
GTATCTT50.045.07
CTCTCAT50.045.028
TTTTCCT50.045.023
GTTTTCC50.045.022
CATCACC50.045.032
AGCAGTT50.045.018
AGTATCT50.045.06
AGCAGCA50.045.015
TTCCTCT50.045.025
TCAGCAG50.045.013
Produced by FastQC (version 0.11.2)