##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_3_82.3410000000ab13.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 33 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.45454545454545 0.0 0.0 0.0 0.0 0.0 2 38.0 0.0 0.0 0.0 0.0 0.0 3 37.81818181818182 0.0 0.0 0.0 0.0 0.0 4 37.63636363636363 0.0 0.0 0.0 0.0 0.0 5 37.81818181818182 0.0 0.0 0.0 0.0 0.0 6 39.878787878787875 0.0 0.0 0.0 0.0 0.0 7 39.39393939393939 0.0 0.0 0.0 0.0 0.0 8 39.63636363636363 0.0 0.0 0.0 0.0 0.0 9 39.63636363636363 0.0 0.0 0.0 0.0 0.0 10 39.303030303030305 0.0 0.0 0.0 0.0 0.0 11 39.75757575757576 0.0 0.0 0.0 0.0 0.0 12 38.81818181818182 0.0 0.0 0.0 0.0 0.0 13 39.63636363636363 0.0 0.0 0.0 0.0 0.0 14 39.75757575757576 0.0 0.0 0.0 0.0 0.0 15 39.75757575757576 0.0 0.0 0.0 0.0 0.0 16 39.878787878787875 0.0 0.0 0.0 0.0 0.0 17 39.57575757575758 0.0 0.0 0.0 0.0 0.0 18 39.81818181818182 0.0 0.0 0.0 0.0 0.0 19 39.81818181818182 0.0 0.0 0.0 0.0 0.0 20 39.878787878787875 0.0 0.0 0.0 0.0 0.0 21 38.93939393939394 0.0 0.0 0.0 0.0 0.0 22 38.36363636363637 0.0 0.0 0.0 0.0 0.0 23 39.36363636363637 0.0 0.0 0.0 0.0 0.0 24 39.63636363636363 0.0 0.0 0.0 0.0 0.0 25 39.121212121212125 0.0 0.0 0.0 0.0 0.0 26 39.93939393939394 0.0 0.0 0.0 0.0 0.0 27 39.878787878787875 0.0 0.0 0.0 0.0 0.0 28 39.57575757575758 0.0 0.0 0.0 0.0 0.0 29 39.42424242424242 0.0 0.0 0.0 0.0 0.0 30 38.81818181818182 0.0 0.0 0.0 0.0 0.0 31 39.0 0.0 0.0 0.0 0.0 0.0 32 39.303030303030305 0.0 0.0 0.0 0.0 0.0 33 39.57575757575758 0.0 0.0 0.0 0.0 0.0 34 39.57575757575758 0.0 0.0 0.0 0.0 0.0 35 39.75757575757576 0.0 0.0 0.0 0.0 0.0 36 39.06060606060606 0.0 0.0 0.0 0.0 0.0 37 38.878787878787875 0.0 0.0 0.0 0.0 0.0 38 39.06060606060606 0.0 0.0 0.0 0.0 0.0 39 38.63636363636363 0.0 0.0 0.0 0.0 0.0 40 39.18181818181818 0.0 0.0 0.0 0.0 0.0 41 39.39393939393939 0.0 0.0 0.0 0.0 0.0 42 39.45454545454545 0.0 0.0 0.0 0.0 0.0 43 39.0 0.0 0.0 0.0 0.0 0.0 44 39.515151515151516 0.0 0.0 0.0 0.0 0.0 45 39.42424242424242 0.0 0.0 0.0 0.0 0.0 46 39.36363636363637 0.0 0.0 0.0 0.0 0.0 47 39.515151515151516 0.0 0.0 0.0 0.0 0.0 48 39.18181818181818 0.0 0.0 0.0 0.0 0.0 49 38.75757575757576 0.0 0.0 0.0 0.0 0.0 50 38.75757575757576 0.0 0.0 0.0 0.0 0.0 51 38.93939393939394 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2105 1 0.0 2105 2 0.0 2105 3 0.0 2105 4 0.0 2105 5 0.0 2105 6 0.0 2105 7 -1.333333333333334 2105 8 -1.333333333333334 2105 9 -1.333333333333334 2105 10 -1.333333333333334 2105 11 0.0 2105 12 0.0 2105 13 0.0 2105 14 0.0 2105 15 0.0 2105 16 0.0 2105 17 0.0 2105 18 0.0 2105 19 0.0 2105 20 0.0 2105 21 0.0 2105 22 0.0 2105 23 0.0 2105 24 0.0 2105 25 0.0 2105 26 0.0 2105 27 0.0 2105 28 0.0 2105 29 0.0 2105 30 0.0 2105 31 0.0 2105 32 0.6666666666666661 2105 33 0.0 2105 34 0.0 2105 35 0.0 2105 36 0.0 2105 37 -1.333333333333334 2105 38 0.0 2105 39 -1.333333333333334 2105 40 0.0 2105 41 0.0 2105 42 0.0 2105 43 0.0 2105 44 0.0 2105 45 0.0 2105 46 0.0 2105 47 0.0 2105 48 0.0 2105 49 0.0 2105 50 0.0 2105 51 -1.333333333333334 1209 1 0.0 1209 2 0.0 1209 3 0.0 1209 4 0.0 1209 5 0.0 1209 6 0.0 1209 7 0.6666666666666661 1209 8 0.6666666666666661 1209 9 0.6666666666666661 1209 10 0.6666666666666661 1209 11 0.0 1209 12 0.0 1209 13 0.0 1209 14 0.0 1209 15 0.0 1209 16 0.0 1209 17 0.0 1209 18 0.0 1209 19 0.0 1209 20 0.0 1209 21 0.0 1209 22 0.0 1209 23 0.0 1209 24 0.0 1209 25 0.0 1209 26 0.0 1209 27 0.0 1209 28 0.0 1209 29 0.0 1209 30 0.0 1209 31 0.0 1209 32 0.6666666666666661 1209 33 0.0 1209 34 0.0 1209 35 0.0 1209 36 0.0 1209 37 0.6666666666666661 1209 38 0.0 1209 39 0.6666666666666661 1209 40 0.0 1209 41 0.0 1209 42 0.0 1209 43 0.0 1209 44 0.0 1209 45 0.0 1209 46 0.0 1209 47 0.0 1209 48 0.0 1209 49 0.0 1209 50 0.0 1209 51 0.6666666666666661 2214 1 0.0 2214 2 0.0 2214 3 0.0 2214 4 0.0 2214 5 0.0 2214 6 0.0 2214 7 0.6666666666666661 2214 8 0.6666666666666661 2214 9 0.6666666666666661 2214 10 0.6666666666666661 2214 11 0.0 2214 12 0.0 2214 13 0.0 2214 14 0.0 2214 15 0.0 2214 16 0.0 2214 17 0.0 2214 18 0.0 2214 19 0.0 2214 20 0.0 2214 21 0.0 2214 22 0.0 2214 23 0.0 2214 24 0.0 2214 25 0.0 2214 26 0.0 2214 27 0.0 2214 28 0.0 2214 29 0.0 2214 30 0.0 2214 31 0.0 2214 32 -1.333333333333334 2214 33 0.0 2214 34 0.0 2214 35 0.0 2214 36 0.0 2214 37 0.6666666666666661 2214 38 0.0 2214 39 0.6666666666666661 2214 40 0.0 2214 41 0.0 2214 42 0.0 2214 43 0.0 2214 44 0.0 2214 45 0.0 2214 46 0.0 2214 47 0.0 2214 48 0.0 2214 49 0.0 2214 50 0.0 2214 51 0.6666666666666661 >>END_MODULE >>Per sequence quality scores pass #Quality Count 28 1.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 1.0 37 0.0 38 0.0 39 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.45454545454545 18.181818181818183 12.121212121212121 24.242424242424242 2 18.181818181818183 6.0606060606060606 57.57575757575758 18.181818181818183 3 12.121212121212121 33.33333333333333 39.39393939393939 15.151515151515152 4 18.181818181818183 12.121212121212121 30.303030303030305 39.39393939393939 5 6.0606060606060606 39.39393939393939 36.36363636363637 18.181818181818183 6 30.303030303030305 27.27272727272727 21.21212121212121 21.21212121212121 7 21.21212121212121 45.45454545454545 15.151515151515152 18.181818181818183 8 21.21212121212121 36.36363636363637 18.181818181818183 24.242424242424242 9 33.33333333333333 21.21212121212121 18.181818181818183 27.27272727272727 10 9.090909090909092 33.33333333333333 36.36363636363637 21.21212121212121 11 21.21212121212121 33.33333333333333 24.242424242424242 21.21212121212121 12 6.0606060606060606 30.303030303030305 33.33333333333333 30.303030303030305 13 15.151515151515152 39.39393939393939 24.242424242424242 21.21212121212121 14 24.242424242424242 15.151515151515152 30.303030303030305 30.303030303030305 15 21.21212121212121 27.27272727272727 27.27272727272727 24.242424242424242 16 36.36363636363637 18.181818181818183 18.181818181818183 27.27272727272727 17 24.242424242424242 33.33333333333333 21.21212121212121 21.21212121212121 18 30.303030303030305 27.27272727272727 30.303030303030305 12.121212121212121 19 24.242424242424242 30.303030303030305 33.33333333333333 12.121212121212121 20 12.121212121212121 15.151515151515152 27.27272727272727 45.45454545454545 21 21.21212121212121 21.21212121212121 33.33333333333333 24.242424242424242 22 18.181818181818183 27.27272727272727 33.33333333333333 21.21212121212121 23 27.27272727272727 21.21212121212121 39.39393939393939 12.121212121212121 24 33.33333333333333 6.0606060606060606 21.21212121212121 39.39393939393939 25 9.090909090909092 36.36363636363637 27.27272727272727 27.27272727272727 26 21.21212121212121 27.27272727272727 36.36363636363637 15.151515151515152 27 18.181818181818183 18.181818181818183 45.45454545454545 18.181818181818183 28 30.303030303030305 15.151515151515152 30.303030303030305 24.242424242424242 29 24.242424242424242 21.21212121212121 33.33333333333333 21.21212121212121 30 15.151515151515152 36.36363636363637 36.36363636363637 12.121212121212121 31 39.39393939393939 21.21212121212121 27.27272727272727 12.121212121212121 32 21.21212121212121 21.21212121212121 30.303030303030305 27.27272727272727 33 6.0606060606060606 30.303030303030305 45.45454545454545 18.181818181818183 34 30.303030303030305 27.27272727272727 15.151515151515152 27.27272727272727 35 27.27272727272727 15.151515151515152 21.21212121212121 36.36363636363637 36 30.303030303030305 33.33333333333333 24.242424242424242 12.121212121212121 37 21.21212121212121 39.39393939393939 24.242424242424242 15.151515151515152 38 18.181818181818183 24.242424242424242 30.303030303030305 27.27272727272727 39 18.181818181818183 18.181818181818183 36.36363636363637 27.27272727272727 40 21.21212121212121 30.303030303030305 27.27272727272727 21.21212121212121 41 12.121212121212121 27.27272727272727 36.36363636363637 24.242424242424242 42 30.303030303030305 24.242424242424242 18.181818181818183 27.27272727272727 43 21.21212121212121 36.36363636363637 30.303030303030305 12.121212121212121 44 9.090909090909092 30.303030303030305 36.36363636363637 24.242424242424242 45 36.36363636363637 18.181818181818183 42.42424242424242 3.0303030303030303 46 18.181818181818183 30.303030303030305 36.36363636363637 15.151515151515152 47 15.151515151515152 42.42424242424242 18.181818181818183 24.242424242424242 48 21.21212121212121 30.303030303030305 30.303030303030305 18.181818181818183 49 21.21212121212121 30.303030303030305 27.27272727272727 21.21212121212121 50 9.090909090909092 12.121212121212121 39.39393939393939 39.39393939393939 51 15.151515151515152 18.181818181818183 39.39393939393939 27.27272727272727 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.5 31 1.0 32 0.5 33 0.0 34 0.5 35 1.0 36 1.0 37 1.0 38 5.5 39 10.0 40 7.0 41 4.0 42 3.5 43 3.0 44 3.5 45 4.0 46 2.0 47 0.0 48 1.0 49 2.0 50 2.0 51 2.0 52 2.5 53 3.0 54 2.0 55 1.0 56 0.5 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.5 65 1.0 66 0.5 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 33.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCATAAACACGATCATGTTGTTGTTGTTAGAAAGGAGTTTCCTCTTAGATC 1 3.0303030303030303 No Hit CCTCTCAAGAAATTTTAGCCTCCCATTGTAACCATACCATATAGGACAATG 1 3.0303030303030303 No Hit CTCTCCACCTCCAAAGTGATGGTCTTTCCGGTGAGGGTCTTCACGAAGATC 1 3.0303030303030303 No Hit GGCAATGGATCTAGCTAAACACACAAGACTTCAAATATTGGGATGCATACT 1 3.0303030303030303 No Hit ATTCACAAGTCACCTCTTCATATCCCTGAAGTTCACATCCCCGAGGATGTT 1 3.0303030303030303 No Hit AGGGAGCTTGACTGTGAGACAGACATGTCGAGCAGGGACAAAAGTCGGGAC 1 3.0303030303030303 No Hit GGTGAGTAATGCCTAGGAATCTGCCTGGTAGTGGGGGATAACCTGTCTCTT 1 3.0303030303030303 No Hit TTTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACA 1 3.0303030303030303 No Hit CTTGGGACCTACTCCAGCCCCAGGATGCGACGAGCCGACATCGAGGTGCCA 1 3.0303030303030303 No Hit ATTCTAGAGAAGGAGGTGGGTAGTTTTACAAAACGACGACCCTTTGAGTTT 1 3.0303030303030303 No Hit ACAACAACAACAACAACAAACCTACCCAAAGCTTCAACCAAGTCTTGATGG 1 3.0303030303030303 No Hit GTGTTTTCGTTCTTCTCATCTTATTTTTTTGCTGCTGTATTTGTTCCTGTC 1 3.0303030303030303 No Hit GGATAGATGCATCTATAGTTTTTCCATCTTTGACATAATCAAATTTGATGC 1 3.0303030303030303 No Hit GTATCTGATCGTCTTCGAGCCCCCAACTTTTGTTCTTGATTAATGAAAACA 1 3.0303030303030303 No Hit GAACTTGTCATTCCCGTTTTCCTGAATAGCTTTCTGTAGAAGCTTGATAGT 1 3.0303030303030303 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 1 3.0303030303030303 No Hit CCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCT 1 3.0303030303030303 No Hit GTCTTAGGTAATACAGTATTAGTGATCAGGATCAGTAAGGGTACCTGTCTC 1 3.0303030303030303 No Hit GTACTGGCACCTGAGTAGGTCGTTGTTCGTGAAACGATGACTGAATCCGCG 1 3.0303030303030303 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC 1 3.0303030303030303 No Hit CTATTCAGCAAAGAGGAATTGTACCCTTTTGCAAGGGTCTTGATGCTGTCT 1 3.0303030303030303 No Hit GTGCACCATACTTGAGGAAAATAGATCTGAGTTGTTACAAAGGATACTCCT 1 3.0303030303030303 No Hit ATTTGGAAATGAAGGAATGAGAGTTGGGGCGTATCAACATGATAGACACTA 1 3.0303030303030303 No Hit TCTTAAGGCTATATGCTTGCCCTGAGCTAAAGACATTACCTGGAGAAATAT 1 3.0303030303030303 No Hit GTAAAAAGACACAGTACCACAAGGCTTGCATTACGTTCTGCAACAAATGCT 1 3.0303030303030303 No Hit CTACTTAGGGAATCACTGTTTTGTTTTCTTTTCTTCCCGGTACTAAGACTG 1 3.0303030303030303 No Hit AGTCAGCTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAA 1 3.0303030303030303 No Hit CTTCTTAGGTCTATCGGGTGATTCCAAGAACACGAATGCAGGAGTTAAGAC 1 3.0303030303030303 No Hit GTGCTCTACCTCGGGCCATCGACATCATACGCTCTACTGAAATAGATTTCG 1 3.0303030303030303 No Hit GTTCTTAATGTCATCCCTGAGTTCCAATTTTCTACAAGTGTGACTGTCTCT 1 3.0303030303030303 No Hit TCTCCGTCGAGTAGTAGTACTAGTCGATCATCTGCGATTCCAGATTTCTCT 1 3.0303030303030303 No Hit TGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATCTGTCTCTTATACA 1 3.0303030303030303 No Hit GACCAACAGTGAGTCCATACTGAGGAAGCTCATGTGCGTAAGCCGAAGCTT 1 3.0303030303030303 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 3.0303030303030303 35 0.0 0.0 3.0303030303030303 36 0.0 0.0 6.0606060606060606 37 0.0 0.0 6.0606060606060606 38 0.0 0.0 9.090909090909092 39 0.0 0.0 9.090909090909092 >>END_MODULE >>Kmer Content pass >>END_MODULE