FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_80.3410000000aaf8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_80.3410000000aaf8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTGGATCAATCTTTTGTTCCACAGTGATTTTCTTCCCTGCTCCAATGA11.7857142857142856No Hit
TATCAACGCAGAGTACGGGTCCAGAACGCGCCACTACACATCGCCCTATTC11.7857142857142856No Hit
GGTTAACACGTCTTAGAGGAGAGATATCAACAGCTTGTCTCCTAACAACAC11.7857142857142856No Hit
AGTCTGCGCTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTA11.7857142857142856No Hit
AATGTGGATATCAGGAAGGATCTGTACGGTAACCTGTCTCTTATACACATC11.7857142857142856No Hit
ATCAACTAGCAAACAAAAATGGCCAAGATCAGTCAAGACGCTGTCTCTTAT11.7857142857142856No Hit
GTCCATACTGCTGCTTCAGAAACGGTAGGTTCTCAATGTGTCCTTGGAAGA11.7857142857142856No Hit
TCTTTGTCCCGTCCTTCTGATCATGGAGTTCCTAAGTAATAGTTGCGATTC11.7857142857142856No Hit
CCTTGCTAGCGACCCATTGATCCTCATACGGTCAGAGAGAATGGAAGGGGT11.7857142857142856No Hit
ATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTT11.7857142857142856No Hit
GAAAAGGATTGGCTCTGAGGGCTGGGCTCGGGGGTCCCAGTTCCGAACCCG11.7857142857142856No Hit
CTAGAATGTTCTCTGGTTTCAAATCTCGGTATATAACACCAAGCATGTGTA11.7857142857142856No Hit
TGTCAAAGTGGAGGGAAGATCTCTAGCAAAATCATTTCGAGTTGAGAATGT11.7857142857142856No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC11.7857142857142856No Hit
TCCATACACCGAAAGAAGATGATGTATACATTGCACCTGCTCAGGTTGTTA11.7857142857142856No Hit
TTCATATGGAAGGAGGAGGATGGTGCACTGATATAGCTACATGCGTTCAAC11.7857142857142856No Hit
GAACAGAAGAGCAACTAGCTTCTGGAGATAGACAAGGTGTGCTAGACAATA11.7857142857142856No Hit
AGTATAAACCCACGAAGCAGATCGACAATGATTTCGTGGCGTCTTTTCCTT11.7857142857142856No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGC11.7857142857142856No Hit
GTAATGATACAAACAACAATGATTCGAGTAACTTCGAATGTAACATCTGCT11.7857142857142856No Hit
CAGTACGACGATGTTATGAAATTGGACTTTGGAACACATATTGATGGGCAT11.7857142857142856No Hit
ACTAAGATCTTGGAGGTGTGAAACGCGAACACTTGTTCCTGTCTCTTATAC11.7857142857142856No Hit
ACCCTAGACTATGCTTGTGGAAATGGAGCAGATTGTAATCCTACTAAACCA11.7857142857142856No Hit
CCTCAAGAGGAAGAGTTGCTATTAATGGAGCACATACTTCTAGCGTCATCG11.7857142857142856No Hit
GTTCCAGTGTAGAGGAGATTTAAGTAACCATGACTGTTACAACTGTGTTAG11.7857142857142856No Hit
GATGTAACAGTGATTCTTGACACTCCCTTAACATATAGTCTGGACGCTGTC11.7857142857142856No Hit
GTGGTGATCGTCTCACTCGTGCCTCCAAGGTGTTGGAACAGCTCAGTGGTC11.7857142857142856No Hit
ATCCAACCTAGTGTCATTTTTTCGTCCCGTACTCTGCGTTGATACCTGTCT11.7857142857142856No Hit
CATCACTACTGAGTACATGACCTACATGTTCAAGTACGACAGTGTCTGTCT11.7857142857142856No Hit
CTCTATAACCGAAAACAATAACGAAGACAGAAAGATAGGACCATTCTTAGC11.7857142857142856No Hit
AAGCCATAGTAACCAAGGTAGCATGGTACTGGTGTAAAAGCTGTCTCTTAT11.7857142857142856No Hit
GGTTATGGGAATGATGTTGCAGCCTCATCCTCAGCATTGATTGGTATGAGT11.7857142857142856No Hit
TCTTAAGGCCACTCAAGGTGAAGCTATCAGGAACTATTACCATGATATGCA11.7857142857142856No Hit
GATCTTGAGCTTCATCTGGGCGCGCTTGCGCCTGTCTCTTATACACATCTC11.7857142857142856No Hit
ATTTTAAACTTCACTCTGCTCCTTTGATCCATTGGCTTCTCATTATTCTCA11.7857142857142856No Hit
CCTTTGGGATATCTCAGACATGCTTTGTTTGAGAACATTTACCAAACTTGA11.7857142857142856No Hit
CCTCTTTCACTCTCTAGGCTTGTAGAGTTCATGACTGGCAAGGATGACAAG11.7857142857142856No Hit
GTACCTGTACCACAAAATGTATGATCTGCAAGCAGCAAGTGCATCAAGATG11.7857142857142856No Hit
ATTGAGGGATACATCGCTTTATCAACCCTTGAGCGTAGAGCAGCAGCAAAG11.7857142857142856No Hit
GTTAGATTGTTGACCAAGAAAAAACATATTGTTGAGATTGAAAGATATATT11.7857142857142856No Hit
GTCCCAAAAGCATTGAAAGTGATAGCAGCAATCGTATTCACTTGTTCTTGC11.7857142857142856No Hit
TTCATCATCATCATCTCTGTTTGTTGGGTTCAAGAATTTAAGTGATTGTTT11.7857142857142856No Hit
GAGCTAAATCTAAGGCTGAAACCAGGAGCACAAAGCTCTCTATGACTAAGG11.7857142857142856No Hit
TGTCAACAGTGGTCCACGTGAAGATGCTACCAGAATTGGATCTGCTGGTGT11.7857142857142856No Hit
GTTTCACAGTCTGAATTCGTTCATACTTACACATGCATGGCTTAATCTTTG11.7857142857142856No Hit
TATACGTTCAGCTGCCATGAAGGACCGAGCAGTGATGGAGAAGGGATTAAA11.7857142857142856No Hit
TCTCTATACCCTGAACGACGTCCATTCCCTTCACAACTCTTCCAAAAACAG11.7857142857142856No Hit
GGGATTTTACCGAAAGGCTGAATCGCGAGATACTCAGGCTGTCTCTGTTCT11.7857142857142856No Hit
CTGTAAAATGATCCGGAGGATAGAATTGAGTGCAGGTTCTGTCTCTTATAC11.7857142857142856No Hit
CCAATCACCATTGTAACTTTCAGAAACAACAGAGCCTATAATTCTGTCTTG11.7857142857142856No Hit
GTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGT11.7857142857142856No Hit
GTCTTGTAGGCCGCCGTGGTCTTACCGGCGCAGCATTCACTGCTAAGGTGA11.7857142857142856No Hit
GATCAGGACAGTCCAACACGTAGTTATGATCGTATCCGATACCAACCTCTC11.7857142857142856No Hit
GTACGGGCTTCTTCTTCTTCTTCAACTCAAAAAACTTCTCTCTCTCTCTCT11.7857142857142856No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTCTGTCTCTTA11.7857142857142856No Hit
AGTGTAACCAAATTTTGACTCGTTATAGTCAATCACACAAAAGCTGTCTCT11.7857142857142856No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC50.045.042
GATATCA50.045.07
TGTCTCT50.045.035
AGGATCT50.045.017
CCTGTCT50.045.033
TACGGTA50.045.025
TCAGGAA50.045.011
GGATCTG50.045.018
CTCTTAT50.045.038
AGGAAGG50.045.013
AACCTGT50.045.031
TTATACA50.045.041
GGATATC50.045.06
TACACAT50.045.044
ACACATC50.045.045
ACCTGTC50.045.032
ATGTGGA50.045.02
ATCAGGA50.045.010
TAACCTG50.045.030
TCTGTAC50.045.021
Produced by FastQC (version 0.11.2)