FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_79.3410000000aaeb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_79.3410000000aaeb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC23.9215686274509802No Hit
GTTAACGAACCAGATAGGTTGTTATGCTGAAGATCAAGAAAAGTGAGGGTA11.9607843137254901No Hit
ACTCTGTTTTCATGGCTGTTTACAATACCAAACTCTGTTTCTGTCTCTTAT11.9607843137254901No Hit
GACTAAGACAGTGTCCGTCTATCACAGCTGCTCCAACATGTCTCCCTTCTA11.9607843137254901No Hit
GTAAAAGGGACTCCTGATCTTCATTGTCCATGCTTGAAGAAAGTTGAGTTA11.9607843137254901No Hit
GTGTTCTTGTACTCTTCTGCCATACTTAATCTTGATTCTGTCTCTTATACA11.9607843137254901No Hit
GTTCGGACTTAAGATGCTCTCTGTTTTACTTTCGTGTGTTGTCTTTCTTAA11.9607843137254901No Hit
GCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGG11.9607843137254901No Hit
GTCAAATACCTTCTGATGGTTAGACAGAAGGTGAAGGAACCCAAGGTTGAT11.9607843137254901No Hit
AGTTTCTTCCATTGGCTTAGTAATAAAGATTCATGGCGATAGATGCATGGT11.9607843137254901No Hit
CCCTAAAATACGTAGAGAGATCAGATACCTGAAAACAACCAATCAATCACT11.9607843137254901No Hit
ACTCAAAACATGGCGAGGGGTTTGAAGAAGCATCTGAAGAGGCTTAATGCG11.9607843137254901No Hit
CTACTAAGGGAATCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTT11.9607843137254901No Hit
AACCATAGCAACTTCTTTCCTCATGGGATCAACTAATCCTTCAAGTTCCTC11.9607843137254901No Hit
CTCAAAATTCCTTATCTCGTCGATTAATTTCTCTCAGATCATGTTGACTGA11.9607843137254901No Hit
ACTCAGGATGATCTCCTCCAGATACATCCATTTGCCTAAGATTTTCATGAA11.9607843137254901No Hit
TCTTTGTTCTTGTTCTAGTTTGTTCGTTAATTCTTGAGCCTCAAATTGAGA11.9607843137254901No Hit
CCTTTTATCTCTCTTCTGTTGCAATTTGATTTTTAAAAAATCTCTCTCTCG11.9607843137254901No Hit
GCTCATCTTTTTATTCGTTTTCGAATCGATTCGTTATCTCTGTCTCTTATA11.9607843137254901No Hit
CTCTTACTATAGGTTTCTTGGTGGTTTCACGCGGTTACAAGACTGTCTCTT11.9607843137254901No Hit
AACAAGAAGTTTATCATCCATCGCCAAACACTTAACCAGAATTTTCTTTGA11.9607843137254901No Hit
CTTCCTCATCATCAGTCTCTACTTCATCTCCATTGCTTTCCTCTCCATCTG11.9607843137254901No Hit
GTTTAGGAGGCGACCGCCCCAGTCAAACTGCCCAAACTGAAACTGTCCCCG11.9607843137254901No Hit
CAAGCAACAGCTCTCGTTGAAGAACGAGGAAGAAGAAGGACGAAGCAGTAA11.9607843137254901No Hit
GCCCTGTACGCATCTTTGGCTAACTTGTTCAGATTGTAGTCCTTGGCAACA11.9607843137254901No Hit
GCTCTGAATAGGGATCTTGTCTCCATCCAATCTATCATGTACCTGTCTCTT11.9607843137254901No Hit
GTGTGTCTCCTTCGTCCATGACTTTGTCTCTTGCTTCTGTTAAATGACTTG11.9607843137254901No Hit
CCACAGGAGTATCAAGAGACGGGATTCGAAGGAAATGGTACTGTCTCTTAT11.9607843137254901No Hit
GGATATGAGAGGGGTGGGCAGACACGTGAACATGTTCAACTTGCCAAAACA11.9607843137254901No Hit
TTCGAAAACAGTGAATGAAAAGAACTCCAGAGCTGAAGTCAAAGAGAAGGA11.9607843137254901No Hit
TCATTACCATTCATCCAATGAGATGGGGAGCCACTCTTATAAACTCTTTTC11.9607843137254901No Hit
TATCAACGCAGAGTACGGGAGGGTTTCTTTGATCAAACCAGAGACTGTCTC11.9607843137254901No Hit
ATTTTTATATTTGCCGAGTCAGTTAGCTTTTTGTTACTGTGATGTACATGA11.9607843137254901No Hit
GGTCTTATGATTTTGATCTTATGATGATTTTAATGCTGTCTCTTATACACA11.9607843137254901No Hit
CCTTCTATGAGAAAAGAGACAAGACCATTGAGACCAACAAGACTGATAACC11.9607843137254901No Hit
GAACTGGTGTTATACAGGAATGTAAGAGGAGAAGATACTTTGAGAATAAAC11.9607843137254901No Hit
ATTTAGAGGAGTGTTTGGTTCTTTGGATAACAATATCCCCTGTCTCTTATA11.9607843137254901No Hit
ACCACAGTGTGTACAATTTCACCACCCTCCTCATATTGCTATTAACCCTTG11.9607843137254901No Hit
ACCAAAAAGGATCCACCAGCATGTCAGAACGCCTAGGCTGTCTCTTATACA11.9607843137254901No Hit
ATGCAAGGAATGGGTCCCTGTGGGATTGGATTCAGAACAAATTGGCCATCA11.9607843137254901No Hit
GACAATGCTTTGATCAATACAGGAAATGCCATCCTGTCGCTTATACACATC11.9607843137254901No Hit
ACAACACATCGTCGGCGTTTTCTTCCCGGAGTAAAGTCACAGGAGAAGACC11.9607843137254901No Hit
ATCTCATACTCAACAAAAGCATGGACCTTGTTACTGAAAAGCCTGTCTCTT11.9607843137254901No Hit
CAGTCGAGCGAGTTTCTGGATCAAGGTCACCTAATTTACTAGTCTCTGGTT11.9607843137254901No Hit
GTATAGCCATGAAGCAAAGTTCCCATTTTCACCAACTAAAGCAATAGGCAC11.9607843137254901No Hit
GTATGTACCTACCCATCTATCTATGACAGTATCTATCTATCCTGTCTCTTA11.9607843137254901No Hit
AGTTCACGCTTGTCTCAGCTTGAACTCTACTGCTACAACCTTGATAAGACC11.9607843137254901No Hit
TCTCTCAGGTTGCTTTGGGAGAGAGTAAGAACAATGTAACCGAACCAATCC11.9607843137254901No Hit
TTCCTCAACAGTGCCAAGTGATTCAGATACACATGATGATTTCAAGCCTAC11.9607843137254901No Hit
AGGAAAACAAAAAACAACGAATACATCGAAACAAGACAAAACGAGAGAAAA11.9607843137254901No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTC50.045.022
ACTGTCT50.045.042
CAAGACT50.045.038
TTCACGC50.045.026
CTATAGG50.045.07
TTTCTTG50.045.014
TTACTAT50.045.04
TGTCTCT50.045.044
CACGCGG50.045.028
ATAGGTT50.045.09
GCGGTTA50.045.031
CTCTTAC50.045.01
GTTTCTT50.045.013
TTACAAG50.045.035
TTTCACG50.045.025
GGTTTCT50.045.012
GTTTCAC50.045.024
GGTTTCA50.045.023
CTTGGTG50.045.017
GGTTACA50.045.033
Produced by FastQC (version 0.11.2)