##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_3_78.3410000000aade.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27 Sequences flagged as poor quality 0 Sequence length 51 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.370370370370374 0.0 0.0 0.0 0.0 0.0 2 37.370370370370374 0.0 0.0 0.0 0.0 0.0 3 37.55555555555556 0.0 0.0 0.0 0.0 0.0 4 38.0 0.0 0.0 0.0 0.0 0.0 5 37.77777777777778 0.0 0.0 0.0 0.0 0.0 6 39.7037037037037 0.0 0.0 0.0 0.0 0.0 7 39.22222222222222 0.0 0.0 0.0 0.0 0.0 8 38.7037037037037 0.0 0.0 0.0 0.0 0.0 9 39.2962962962963 0.0 0.0 0.0 0.0 0.0 10 39.48148148148148 0.0 0.0 0.0 0.0 0.0 11 38.55555555555556 0.0 0.0 0.0 0.0 0.0 12 39.2962962962963 0.0 0.0 0.0 0.0 0.0 13 39.370370370370374 0.0 0.0 0.0 0.0 0.0 14 39.851851851851855 0.0 0.0 0.0 0.0 0.0 15 39.77777777777778 0.0 0.0 0.0 0.0 0.0 16 39.55555555555556 0.0 0.0 0.0 0.0 0.0 17 39.77777777777778 0.0 0.0 0.0 0.0 0.0 18 39.629629629629626 0.0 0.0 0.0 0.0 0.0 19 39.77777777777778 0.0 0.0 0.0 0.0 0.0 20 38.7037037037037 0.0 0.0 0.0 0.0 0.0 21 39.074074074074076 0.0 0.0 0.0 0.0 0.0 22 39.7037037037037 0.0 0.0 0.0 0.0 0.0 23 39.7037037037037 0.0 0.0 0.0 0.0 0.0 24 39.629629629629626 0.0 0.0 0.0 0.0 0.0 25 39.7037037037037 0.0 0.0 0.0 0.0 0.0 26 39.629629629629626 0.0 0.0 0.0 0.0 0.0 27 39.7037037037037 0.0 0.0 0.0 0.0 0.0 28 39.77777777777778 0.0 0.0 0.0 0.0 0.0 29 39.77777777777778 0.0 0.0 0.0 0.0 0.0 30 39.851851851851855 0.0 0.0 0.0 0.0 0.0 31 39.851851851851855 0.0 0.0 0.0 0.0 0.0 32 39.7037037037037 0.0 0.0 0.0 0.0 0.0 33 39.7037037037037 0.0 0.0 0.0 0.0 0.0 34 39.851851851851855 0.0 0.0 0.0 0.0 0.0 35 39.851851851851855 0.0 0.0 0.0 0.0 0.0 36 39.77777777777778 0.0 0.0 0.0 0.0 0.0 37 39.55555555555556 0.0 0.0 0.0 0.0 0.0 38 39.7037037037037 0.0 0.0 0.0 0.0 0.0 39 39.77777777777778 0.0 0.0 0.0 0.0 0.0 40 39.77777777777778 0.0 0.0 0.0 0.0 0.0 41 39.77777777777778 0.0 0.0 0.0 0.0 0.0 42 39.77777777777778 0.0 0.0 0.0 0.0 0.0 43 39.55555555555556 0.0 0.0 0.0 0.0 0.0 44 39.55555555555556 0.0 0.0 0.0 0.0 0.0 45 39.333333333333336 0.0 0.0 0.0 0.0 0.0 46 39.7037037037037 0.0 0.0 0.0 0.0 0.0 47 39.77777777777778 0.0 0.0 0.0 0.0 0.0 48 38.7037037037037 0.0 0.0 0.0 0.0 0.0 49 39.370370370370374 0.0 0.0 0.0 0.0 0.0 50 39.55555555555556 0.0 0.0 0.0 0.0 0.0 51 39.0 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2112 1 0.0 2112 2 0.0 2112 3 0.0 2112 4 0.0 2112 5 0.0 2112 6 0.0 2112 7 0.0 2112 8 0.0 2112 9 0.0 2112 10 0.0 2112 11 0.0 2112 12 0.0 2112 13 0.0 2112 14 0.0 2112 15 0.0 2112 16 -1.0 2112 17 0.0 2112 18 0.0 2112 19 0.0 2112 20 0.0 2112 21 1.0 2112 22 0.0 2112 23 0.0 2112 24 0.0 2112 25 0.0 2112 26 0.0 2112 27 0.0 2112 28 0.0 2112 29 0.0 2112 30 0.0 2112 31 0.0 2112 32 0.0 2112 33 0.0 2112 34 0.0 2112 35 0.0 2112 36 0.0 2112 37 0.0 2112 38 0.0 2112 39 0.0 2112 40 -1.0 2112 41 0.0 2112 42 0.0 2112 43 0.0 2112 44 0.0 2112 45 0.0 2112 46 0.0 2112 47 0.0 2112 48 0.0 2112 49 1.0 2112 50 0.0 2112 51 1.0 1209 1 0.0 1209 2 0.0 1209 3 0.0 1209 4 0.0 1209 5 0.0 1209 6 0.0 1209 7 0.0 1209 8 0.0 1209 9 0.0 1209 10 0.0 1209 11 0.0 1209 12 0.0 1209 13 0.0 1209 14 0.0 1209 15 0.0 1209 16 1.0 1209 17 0.0 1209 18 0.0 1209 19 0.0 1209 20 0.0 1209 21 -1.0 1209 22 0.0 1209 23 0.0 1209 24 0.0 1209 25 0.0 1209 26 0.0 1209 27 0.0 1209 28 0.0 1209 29 0.0 1209 30 0.0 1209 31 0.0 1209 32 0.0 1209 33 0.0 1209 34 0.0 1209 35 0.0 1209 36 0.0 1209 37 0.0 1209 38 0.0 1209 39 0.0 1209 40 1.0 1209 41 0.0 1209 42 0.0 1209 43 0.0 1209 44 0.0 1209 45 0.0 1209 46 0.0 1209 47 0.0 1209 48 0.0 1209 49 -1.0 1209 50 0.0 1209 51 -1.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 36 1.0 37 1.0 38 2.0 39 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.22222222222222 29.629629629629626 29.629629629629626 18.51851851851852 2 25.925925925925924 18.51851851851852 37.03703703703704 18.51851851851852 3 0.0 29.629629629629626 44.44444444444444 25.925925925925924 4 11.11111111111111 14.814814814814813 48.148148148148145 25.925925925925924 5 3.7037037037037033 48.148148148148145 40.74074074074074 7.4074074074074066 6 18.51851851851852 59.25925925925925 18.51851851851852 3.7037037037037033 7 11.11111111111111 29.629629629629626 33.33333333333333 25.925925925925924 8 22.22222222222222 22.22222222222222 25.925925925925924 29.629629629629626 9 37.03703703703704 0.0 33.33333333333333 29.629629629629626 10 7.4074074074074066 33.33333333333333 29.629629629629626 29.629629629629626 11 25.925925925925924 22.22222222222222 33.33333333333333 18.51851851851852 12 18.51851851851852 33.33333333333333 29.629629629629626 18.51851851851852 13 25.925925925925924 11.11111111111111 51.85185185185185 11.11111111111111 14 14.814814814814813 18.51851851851852 25.925925925925924 40.74074074074074 15 18.51851851851852 40.74074074074074 29.629629629629626 11.11111111111111 16 29.629629629629626 37.03703703703704 11.11111111111111 22.22222222222222 17 29.629629629629626 18.51851851851852 25.925925925925924 25.925925925925924 18 22.22222222222222 22.22222222222222 22.22222222222222 33.33333333333333 19 25.925925925925924 29.629629629629626 29.629629629629626 14.814814814814813 20 29.629629629629626 18.51851851851852 25.925925925925924 25.925925925925924 21 18.51851851851852 29.629629629629626 29.629629629629626 22.22222222222222 22 18.51851851851852 18.51851851851852 37.03703703703704 25.925925925925924 23 14.814814814814813 37.03703703703704 33.33333333333333 14.814814814814813 24 22.22222222222222 22.22222222222222 29.629629629629626 25.925925925925924 25 18.51851851851852 33.33333333333333 29.629629629629626 18.51851851851852 26 7.4074074074074066 51.85185185185185 25.925925925925924 14.814814814814813 27 25.925925925925924 22.22222222222222 29.629629629629626 22.22222222222222 28 25.925925925925924 22.22222222222222 25.925925925925924 25.925925925925924 29 22.22222222222222 25.925925925925924 29.629629629629626 22.22222222222222 30 18.51851851851852 33.33333333333333 29.629629629629626 18.51851851851852 31 25.925925925925924 33.33333333333333 25.925925925925924 14.814814814814813 32 14.814814814814813 51.85185185185185 18.51851851851852 14.814814814814813 33 18.51851851851852 33.33333333333333 25.925925925925924 22.22222222222222 34 18.51851851851852 18.51851851851852 37.03703703703704 25.925925925925924 35 29.629629629629626 37.03703703703704 14.814814814814813 18.51851851851852 36 14.814814814814813 44.44444444444444 22.22222222222222 18.51851851851852 37 22.22222222222222 25.925925925925924 33.33333333333333 18.51851851851852 38 18.51851851851852 25.925925925925924 37.03703703703704 18.51851851851852 39 3.7037037037037033 25.925925925925924 29.629629629629626 40.74074074074074 40 22.22222222222222 29.629629629629626 33.33333333333333 14.814814814814813 41 7.4074074074074066 33.33333333333333 40.74074074074074 18.51851851851852 42 29.629629629629626 29.629629629629626 22.22222222222222 18.51851851851852 43 7.4074074074074066 25.925925925925924 44.44444444444444 22.22222222222222 44 44.44444444444444 18.51851851851852 25.925925925925924 11.11111111111111 45 7.4074074074074066 29.629629629629626 25.925925925925924 37.03703703703704 46 22.22222222222222 14.814814814814813 40.74074074074074 22.22222222222222 47 11.11111111111111 37.03703703703704 40.74074074074074 11.11111111111111 48 14.814814814814813 37.03703703703704 44.44444444444444 3.7037037037037033 49 25.925925925925924 25.925925925925924 37.03703703703704 11.11111111111111 50 18.51851851851852 40.74074074074074 22.22222222222222 18.51851851851852 51 29.629629629629626 25.925925925925924 40.74074074074074 3.7037037037037033 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.5 29 1.0 30 1.5 31 2.0 32 2.0 33 2.0 34 3.5 35 5.0 36 3.5 37 2.0 38 3.5 39 5.0 40 3.5 41 2.0 42 1.0 43 0.0 44 1.5 45 3.0 46 2.5 47 2.0 48 1.5 49 1.0 50 0.5 51 0.0 52 0.5 53 1.0 54 0.5 55 0.0 56 0.5 57 1.0 58 0.5 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 27.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATCAATTTGTCGGTAGTCTCCTTTTGGTCATATCCTATAGTAAGAGATGAG 1 3.7037037037037033 No Hit TGTCAAGAGTCTGACAAAGCTTAAAAAGAAAAATGCTCTCTGTTTCTGTCT 1 3.7037037037037033 No Hit GTATCAACGCAGAGTACGGGATCTGAGGGAGAAGAAGAAACGTGAAGAGAT 1 3.7037037037037033 No Hit CCATTAAAGCCATGGAGCCTTTCTTCTCTGTTTTATTGCTGTCTCTTATAC 1 3.7037037037037033 No Hit CTTCACATGCGTTTCGGCAGAACATATTGTACACGCTCCTCTTGGCATAAG 1 3.7037037037037033 No Hit TGTCAACACATATAGCAACGTAAGCTAGACCAGAAACTGTTGAGCTTACAT 1 3.7037037037037033 No Hit GGCTAGGTTGTAGAAATGGGTAATGTTACGCATCATCCACACGGACTATGT 1 3.7037037037037033 No Hit CTTCTTACTTAATCAACGTTCTGCATAACATCCTCAGACGTAAACTTAGTA 1 3.7037037037037033 No Hit TCTTAACCTATGTCAAGTTTTCTGGAGGTTGAATGCTTTTAGTTCCATGAT 1 3.7037037037037033 No Hit TGCCTGTTCTTCAAATCAACACTTCTATGTTGTTGAAGTGTTAAATCATTG 1 3.7037037037037033 No Hit GGCGTATATATGTGGATCGAATAATAAACGAGAACGTACGTTGTCGTTGTG 1 3.7037037037037033 No Hit TATCAACGCAGAGTACGGGGGGACAACAATATTCGGAGAAAGTGACGGAGA 1 3.7037037037037033 No Hit GTCTTTACCCTTCTTGTATTGGGTAACCTTGTGCAAAGTATGCTTTTTGCA 1 3.7037037037037033 No Hit AGAGAGTTTCCTTCATCCTAAATACCACAAAACATAAACAAAAGCTCTCTT 1 3.7037037037037033 No Hit TATCAACGCAGAGTACGGGACCATCTGTGGGATTATGCTGTCTCTTATACA 1 3.7037037037037033 No Hit AACACGTCTCAATGGAACACCATTAATCTTGAATGGTCCTGTTACAAGAAG 1 3.7037037037037033 No Hit ATCTTATCCCAGCTTCCCCACCACGAGCTCGGGGAATTCGACATTGATCTG 1 3.7037037037037033 No Hit ACATTACTTAGAGTTATTGGATTGTATGGACCATTACTATCATGAGATTGA 1 3.7037037037037033 No Hit AATGTGCGGTGCTCAGGAAGGTTCTGTGTCTAAGTCTAACAACGAGAATGT 1 3.7037037037037033 No Hit GCTTTACCGGTTTCTGGTAAGGAGAACTAATAGCAAGTTCAATGGTGTGAT 1 3.7037037037037033 No Hit ATTATTAGCTTTTCAGGTTTATGAAACCCTCTGAATTACTTCCTCTTTTAT 1 3.7037037037037033 No Hit TGATAAATGAGCTCTTTCTTGTTGGCGTTTTTTGGAGTTAAGTGCAAGAAG 1 3.7037037037037033 No Hit TAATAATACAGTACTCACACTCACAACGACAACGTACGTTCTCGTTTATTA 1 3.7037037037037033 No Hit CTTTTATAGCATCCCAAGACACAAATCAGAAACTGTCTCTTATACACATCT 1 3.7037037037037033 No Hit ACAAAATGTAAAGCTGCATTTGGCTTTCAAACCTCAAACATGAATTTTAGA 1 3.7037037037037033 No Hit CTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCCTGTCTCT 1 3.7037037037037033 No Hit GTATAAACTTCCTCACCTCCTCTCTCTACCAAACAGATCAAATCACAAAAG 1 3.7037037037037033 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 3.7037037037037037 34 0.0 0.0 3.7037037037037037 35 0.0 0.0 3.7037037037037037 36 0.0 0.0 3.7037037037037037 37 0.0 0.0 3.7037037037037037 38 0.0 0.0 7.407407407407407 39 0.0 0.0 11.11111111111111 >>END_MODULE >>Kmer Content pass >>END_MODULE