FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_78.3410000000aade.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_78.3410000000aade.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAATTTGTCGGTAGTCTCCTTTTGGTCATATCCTATAGTAAGAGATGAG13.7037037037037033No Hit
TGTCAAGAGTCTGACAAAGCTTAAAAAGAAAAATGCTCTCTGTTTCTGTCT13.7037037037037033No Hit
GTATCAACGCAGAGTACGGGATCTGAGGGAGAAGAAGAAACGTGAAGAGAT13.7037037037037033No Hit
CCATTAAAGCCATGGAGCCTTTCTTCTCTGTTTTATTGCTGTCTCTTATAC13.7037037037037033No Hit
CTTCACATGCGTTTCGGCAGAACATATTGTACACGCTCCTCTTGGCATAAG13.7037037037037033No Hit
TGTCAACACATATAGCAACGTAAGCTAGACCAGAAACTGTTGAGCTTACAT13.7037037037037033No Hit
GGCTAGGTTGTAGAAATGGGTAATGTTACGCATCATCCACACGGACTATGT13.7037037037037033No Hit
CTTCTTACTTAATCAACGTTCTGCATAACATCCTCAGACGTAAACTTAGTA13.7037037037037033No Hit
TCTTAACCTATGTCAAGTTTTCTGGAGGTTGAATGCTTTTAGTTCCATGAT13.7037037037037033No Hit
TGCCTGTTCTTCAAATCAACACTTCTATGTTGTTGAAGTGTTAAATCATTG13.7037037037037033No Hit
GGCGTATATATGTGGATCGAATAATAAACGAGAACGTACGTTGTCGTTGTG13.7037037037037033No Hit
TATCAACGCAGAGTACGGGGGGACAACAATATTCGGAGAAAGTGACGGAGA13.7037037037037033No Hit
GTCTTTACCCTTCTTGTATTGGGTAACCTTGTGCAAAGTATGCTTTTTGCA13.7037037037037033No Hit
AGAGAGTTTCCTTCATCCTAAATACCACAAAACATAAACAAAAGCTCTCTT13.7037037037037033No Hit
TATCAACGCAGAGTACGGGACCATCTGTGGGATTATGCTGTCTCTTATACA13.7037037037037033No Hit
AACACGTCTCAATGGAACACCATTAATCTTGAATGGTCCTGTTACAAGAAG13.7037037037037033No Hit
ATCTTATCCCAGCTTCCCCACCACGAGCTCGGGGAATTCGACATTGATCTG13.7037037037037033No Hit
ACATTACTTAGAGTTATTGGATTGTATGGACCATTACTATCATGAGATTGA13.7037037037037033No Hit
AATGTGCGGTGCTCAGGAAGGTTCTGTGTCTAAGTCTAACAACGAGAATGT13.7037037037037033No Hit
GCTTTACCGGTTTCTGGTAAGGAGAACTAATAGCAAGTTCAATGGTGTGAT13.7037037037037033No Hit
ATTATTAGCTTTTCAGGTTTATGAAACCCTCTGAATTACTTCCTCTTTTAT13.7037037037037033No Hit
TGATAAATGAGCTCTTTCTTGTTGGCGTTTTTTGGAGTTAAGTGCAAGAAG13.7037037037037033No Hit
TAATAATACAGTACTCACACTCACAACGACAACGTACGTTCTCGTTTATTA13.7037037037037033No Hit
CTTTTATAGCATCCCAAGACACAAATCAGAAACTGTCTCTTATACACATCT13.7037037037037033No Hit
ACAAAATGTAAAGCTGCATTTGGCTTTCAAACCTCAAACATGAATTTTAGA13.7037037037037033No Hit
CTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCCTGTCTCT13.7037037037037033No Hit
GTATAAACTTCCTCACCTCCTCTCTCTACCAAACAGATCAAATCACAAAAG13.7037037037037033No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)