FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_77.3410000000aac1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_77.3410000000aac1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCCTCCGAGGGTGTCCTCGGATTCCCTTGTCGGATCTGCCTCTTCCA11.7241379310344827No Hit
TTCTTTTGCTGTGCAGAAGCTCTTTAGTTTAATTAGATCCCATTCTGTCTC11.7241379310344827No Hit
CACAGAGTACATGCCATTACCATATTACTAGTCTCACGTACCAATACCTGA11.7241379310344827No Hit
GTTTTGTGTTGCCAATACAACATTCCAATATGAGGCTGTCTCTTATACACA11.7241379310344827No Hit
ATGCTGAATTCCTCTTTTCTAACTCTGTCATTATACGGTCCAATAAAACCT11.7241379310344827No Hit
ATGCAGTACTGAAGGTTGTATGAATGTTGTTTCTTTCTCAAGATGTCTTAG11.7241379310344827No Hit
AAGCAACGTTGTTTAGATTTCTTACATGCAAACGAATGACATAATATCTGT11.7241379310344827No Hit
TTCAACACTTGTGGCTCATTTCTTTGGCATCTTAGCTGTTGTTCTGATGCT11.7241379310344827No Hit
CCTTGGAACAGGGCATCGTAGAGGGTGAAAATCCCGTACTTGCCATGGAAG11.7241379310344827No Hit
TTCTCTTTTGTTTACGTTTGCTTCTTTTTGCTTTATTTTTTTTACCCCCCG11.7241379310344827No Hit
CTTAAGAACGCCGTAATCACGTTCAAGCTGCTTTGTCTTCCACCTGTCTCT11.7241379310344827No Hit
AACAAGAAGAGCTTCTTGTCCTAGATATTGGTTTGCCTATTCCCAGTTTCT11.7241379310344827No Hit
GAAGTAACCCATTGTTGTGATGGTCATTGTTCTGTCCTCTTCTGTTTTAGG11.7241379310344827No Hit
GCATGGGGGAAAGTTCAAGAAGTGGAAGTGATCGTGTGTGTTCTCTCAGCT11.7241379310344827No Hit
GAACTCTGGATCAAGCGAGATGATTTCACCGGAATGGAATTGCTGTCTCTT11.7241379310344827No Hit
CTTGTACTGCATCATCATCATTCATACCATCAAATCCTAAAGCTGTCTCTT11.7241379310344827No Hit
GGGGAATGGAATGCAATGGAATGCAATGGAATTGAATGGAATGGAATGGAA11.7241379310344827No Hit
CCCTTTCTTCTCTTCCTCCTTTACCTCAACTTCAGTAGTTGGCTTCTTCTT11.7241379310344827No Hit
ATACAGGACAATAGACGATAGAAATCCTCCGCAATAAGATGATTCAGTGGT11.7241379310344827No Hit
GACCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAA11.7241379310344827No Hit
GTATATCATCGAACACCTTTGAGTGCATATGCTACCTTGTTCATCTTTACA11.7241379310344827No Hit
CAGTGCTGCCTCATCGAATTCGAAGTAGGAGCCAATATCCTTGTGAGTCAG11.7241379310344827No Hit
ACTCAAAGCTCTTCTTCTTTTGAACCTATGTATCTGTTTTCGCTAGTTTTG11.7241379310344827No Hit
TCACTATAGCTTCGCAGTGACAACCTTGATCGAATGTGTAGGATAGGTGCT11.7241379310344827No Hit
GAGTAGTAATCAGATGTTTCCGCTACAGGATATGTTCATGTACATGCAGAA11.7241379310344827No Hit
CGTTCTTAATGTTGAACGTTGTGCGGATCTCTTCTGGAGTCTTTCCTTTGA11.7241379310344827No Hit
TTGTACAGGAGGACCACCTAAAGACTGCAAAACCTGAACATCATCATCGTC11.7241379310344827No Hit
CCTTTATCCGCATTGACTGGATCCATCTTAGCGGTGAGAGATTTGAGAGAG11.7241379310344827No Hit
GTATCAACGCAGAGTACGGGTTAATGATGCCCCTTTCGATGGTTATGTTGA11.7241379310344827No Hit
ATCATCTTCAACACTCTCATTGTTTACTTAACCAAAGTGTACGTCTCAGGC11.7241379310344827No Hit
CCATGGGCCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACG11.7241379310344827No Hit
CAACAAGAGCAATCAAGAATGAAGAAGAAAAGAGAAGAGTTGCATTTGATG11.7241379310344827No Hit
AGCTAATAGATACTCAATATCCATGTCCATCTCCAAATCCTCATCCTTCAG11.7241379310344827No Hit
CTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTAGCTAAAGGAA11.7241379310344827No Hit
CACATATACCATTCGATCGGCAGTCCTATATGATCTCCTCTAATTTAACAG11.7241379310344827No Hit
TTCTTTGAACTTTCTGCTTTGGCCAACCTTCTTATGGCTGCGCCGGCATGG11.7241379310344827No Hit
CAACAGTTCGGTTTGTGTCACGGATGTTTCATCCTAATATTTATGCAGATG11.7241379310344827No Hit
ATACACAACAACACGAGTGAAGTAGATGTAGCAGATAACCACAATGTAGTA11.7241379310344827No Hit
ATCAGAATCAACACGCTGGTAATGATTCTCAGCTATGGATATCAACAGAGA11.7241379310344827No Hit
GGTAAGTATCCTGGTGTTGCTAATTTACCTCTTAACAACAGGGTTAAAGCT11.7241379310344827No Hit
GTAAGAGACTCTTGTGACCAACTTCTTGATGTCGAAGATTCTGAGACTGCT11.7241379310344827No Hit
CTTACATTCTTTCAGCATTTTTCAACAGCCAGTCGAGTCCTTCATGTAAAC11.7241379310344827No Hit
ATGCTGCATCACAGAGGGGCATGCTGCAGAAGAAGCACGATGGATCCAACT11.7241379310344827No Hit
TACAAATACATACTAAGGGTTTCTTATATGCTCAACACATGAGCGAAACCC11.7241379310344827No Hit
CTAGAAGACCAAGAGTTGAAAAACGCTGTGAAATCCTTCAGCCACTGGCCT11.7241379310344827No Hit
GAAGATAATGATGTAAACAAAGCCGCAATTTCAGAGCTGTCTCTTATACAC11.7241379310344827No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC11.7241379310344827No Hit
ATACATAATGAAGACGGATGATGATGCGTTTGTACGGATTGATGAGCTCCT11.7241379310344827No Hit
CACCATTTTCAATCGAAGAACAACAATCAAACACAAATCCCTGTCTCTTAT11.7241379310344827No Hit
GTCTAATTCCGCGATAAAAGAGCTCTGTTTCCAAACAGATTTGTCCATCAG11.7241379310344827No Hit
ACCATGGGTAATTTCAGTACAAAAGACAGGAATAAAAGGAAACCCCGTACT11.7241379310344827No Hit
CACTCTCTCTCCGATACTCTTCAGATGCACTTCTACGAGACTTGTCCTTAT11.7241379310344827No Hit
AACAAACACAAAACTTGATCTCACGACTCCATCTTCTAAAATTCAGAATCC11.7241379310344827No Hit
AAAGAGCACAAGAAGAGCTAGATGAAGTTGTAGGCAAAGACAACATTGTTG11.7241379310344827No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG11.7241379310344827No Hit
CTCATGAAGTTCAACGTCGATAACCGATGTACCAATATGAGCCGGTCCGGT11.7241379310344827No Hit
TACATCAAGACTGGTTTTGGGAAGGTGATGATAACCACATGAGGAAGCTAG11.7241379310344827No Hit
CCATAATTGTGTAAATGCATCCAATCCGTACCATAAATGTGTTGAATACTG11.7241379310344827No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGAGT50.045.042
TCCTTGT50.045.038
AAGTAGG50.045.023
CCAATAT50.045.032
GCCTCAT50.045.08
TAGGAGC50.045.026
AGCCAAT50.045.030
AATTCGA50.045.017
AGTAGGA50.045.024
TCGAATT50.045.014
GAAGTAG50.045.022
TCGAAGT50.045.020
CGAAGTA50.045.021
TTCGAAG50.045.019
TGCTGCC50.045.04
CCTTGTG50.045.039
GAGCCAA50.045.029
GTGAGTC50.045.043
CAATATC50.045.033
GAGTCAG50.045.045
Produced by FastQC (version 0.11.2)