FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_76.3410000000aab4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_76.3410000000aab4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCACCATCTTTCGGGTCCCAACATATATGCTCTTACGCAGACCCATCCT12.7777777777777777No Hit
CTATAGAATAAGCTGACTCCTCCTACTCCTCCTTTCCGAAAAGACTGTCTC12.7777777777777777No Hit
CCTGAGATCCGATGCGAACAATCAGTCGAAGGAGTAGTCACTGTCTCTTAT12.7777777777777777No Hit
AAAGTCAACAAAACAACCAAAAGAAACCAGAAAAGTTAAAGACAAACCTAT12.7777777777777777No Hit
GGCAAGAACGTGTTCATGCCTCTTGAAGAGACTCCATTGGCTGTCTCTTAT12.7777777777777777No Hit
CATCACCATTTAGTATGATGTTGACTATGTTTTCCATAGATGCCTTTCCTG12.7777777777777777No Hit
GGTCTTAAGACGGTGCTTAAAGCTGACTCTCAGTGTCTATGTGAAGCTGTC12.7777777777777777No Hit
CAGAGAGACTGGACGCAACAAGGCTATTTCTAGTGTTCCCATTCACCTTAG12.7777777777777777No Hit
GTGGTATCAACGCAGAGTACGGGGGGGGCCGGCGATGCGTCCTGGTCGGAT12.7777777777777777No Hit
AAGCAATACGAGCTATGGAAGCGCTTCCTAAAAGAGGCATCACACCTCCAG12.7777777777777777No Hit
TTGCTTACCAAGAAGGTTGTGAAGAAGAGGTCTGCTAAGTTCATCCTGTCT12.7777777777777777No Hit
GTATCAACGCAGAGTACGGGATTATCACCCTCTACGATTGACCTTTCCAAG12.7777777777777777No Hit
CTCTCTTTCCTCTGCAGTTACAGTTCTCTTTCTAGCAGAAGCACTCTCTGT12.7777777777777777No Hit
CACTTGAAGGTATCAAGCAATTCTACGTGAACGTGGAGAAAGAAGCTGTCT12.7777777777777777No Hit
ATTTTCTGATGAGCTGTGAAAAGGGGTGAAATGCTAATCTAATCTGGTGAT12.7777777777777777No Hit
GGTGTTAAGTCTGGCTTGGACGACAGAAGTGATGAGGAATATTGTTAATCC12.7777777777777777No Hit
GGCTACCCCAAAGGCAACGATCGGAATGGCGAACAGAGCGCCCACGATGCC12.7777777777777777No Hit
CATCAATGAAGGTTTACATGGAAATGCAGGGATTTGGCCATAGGCCATCAG12.7777777777777777No Hit
GTATTGGCAGGTATGCTTTCTGTCGACTCTGACGACTTGCTTGAAGGATGT12.7777777777777777No Hit
TCAGTGACAACCTGAGCAATTTGCACCGGGGGCTGGCCGAGTTATGCCAGC12.7777777777777777No Hit
TCATTAGTCAATCCCTGCATCCTTTTGAGGCTGTCTCTTATACACATCTCC12.7777777777777777No Hit
CTGTTCAATGGATTCTCAAAGTCAAAGTCCGTGGCCATTAAATCTTCCACC12.7777777777777777No Hit
ACATTATCGGCGCGGAATCACTTGACCAGTGAGCTACTGTCTCTTATACAC12.7777777777777777No Hit
TTTATCTCCTCATCTTTGTTTCTCCTACAAAGCTTCCTTCTTTGTTTTAGT12.7777777777777777No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT12.7777777777777777No Hit
CTGCGAGTGACTGAAAACAGTAGTGATGTTGAAGCAGCCTTGGCACTTCAC12.7777777777777777No Hit
TTCCAATATGTGGACAAGGAGTCGAAGTCGTTGATGATGCTTCTGTCTCTT12.7777777777777777No Hit
TATCAACGCAGAGTACGGGGTGTGATTTACTGAGTGAAAAAAGCTTATCGC12.7777777777777777No Hit
AGTTTCAAGACATCAAAAGACAGAACATCTCCTACATATGGACGTAAGAGC12.7777777777777777No Hit
TCATCAATGAGAACCAAAATCCAAGTATCTGAAACTGTTTGAGACGATAAC12.7777777777777777No Hit
TTGTTGGAGACCTTGTCAGGAGTAGAAGTGAATACTATCACTCCAGTTACA12.7777777777777777No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA12.7777777777777777No Hit
GGAGACTACTTGCCTATTGTACGTGAGAAGCCTACCATGGCTGTCTCTTAT12.7777777777777777No Hit
GAACCACATCGTCTGATTCGTTTGAATACGATTCAGCAATTTTTCTTGCTT12.7777777777777777No Hit
GATAAGGAAAGACATTGAGTGCCTTTTTGTTGAAGATGTTGAAGACGATGT12.7777777777777777No Hit
TAAAACACCTAACCATTCTTTATCTCCTTCAATTGGTGCATAAAGCTGTCT12.7777777777777777No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)