FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_75.3410000000aaa8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_75.3410000000aaa8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTAAACAGGTCACACAGGGCAGAGGAATAGAGTACAACAACTCTTACA11.1494252873563218No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1494252873563218No Hit
GCTAAGTGAAGAATTTGTCCACTCCAAGGATTTATTTTGAACAGATAGTTC11.1494252873563218No Hit
CTCTAAAATCAAGGTCGTGCGTAAATCCATAGCTCAGGTTTTGACAGTGTC11.1494252873563218No Hit
GAATAGCTCAAAGTTAAAATCGACAAGGAGAGATCTTTGTTTGAGATGTAA11.1494252873563218No Hit
AACCGGAGCTGCCCATTGGAAATGCCTAGGCACAAAGAAGTTAACTTGCAA11.1494252873563218No Hit
GTATGGCACATTGTCTTGATATTGTGAAAGGCTCGAGTCATGTCTGTCTCT11.1494252873563218No Hit
GAACTGGTGCATCGAAATGAAAACGATAGAAATTCCGAGAAGGAAGTTCAT11.1494252873563218No Hit
GTCCATAATCGCACTTGATTCTCTCATAATCTTCTCGTGACCGTTAAGATC11.1494252873563218No Hit
GTCCGGTAGCGATTGAGTAATGGTTTGGGAACGTCATGGTGGGGAAAAACC11.1494252873563218No Hit
GATATCTCCTTCCTCAAAGAAATCATCAGAAAGTGATGTTGTCAAATACGG11.1494252873563218No Hit
TTATTAGGATGTTTTGTCTCTTCATATCGGTTTTTGGTTTCTTCTTATCCT11.1494252873563218No Hit
AACATGTAGTATCCACGCGGAACATCCTTTCCACAAATAGGGAAATTTCCC11.1494252873563218No Hit
CTGCTGCCCTCATCCTGGGCTAGGCAGCTGGGAGCCAAAGTTGAAATACCT11.1494252873563218No Hit
CGAAGAAGAAGAAGAAGAGATCTGAAGTCCCGTACTCTGCGTTGATACCTG11.1494252873563218No Hit
CAAGATATCTTTTGTGAAGGCAATCAAGGTATAAAACCTGTCTCTTATACA11.1494252873563218No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCCTGTCTCTTATACACATCTC11.1494252873563218No Hit
ATATGAACTCTCGCAAACGATAAACCTGTTATCCCTAGCGTCTGTCTCTTA11.1494252873563218No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC11.1494252873563218No Hit
GTCATGGCACCTTATGCGTGTTATGGCGTGCCTATTGAAGCACTGTCTCTT11.1494252873563218No Hit
GATTAAAGATATCTTGGTGAGGTATGATAGGACTCTGCTTGTGGCTGTCTC11.1494252873563218No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA11.1494252873563218No Hit
CTGTTATACCAACCCTTGCAATCAACACGTCTAATGTTTACAAAGTTGTAC11.1494252873563218No Hit
TTATGAATCCAACGATGTGAATTAAACCACTCTTATACCTGTCTCTTATAC11.1494252873563218No Hit
GTCTCATGGGGATACTAAAGTCGTGTTTGGACGCTAAAACCGTGAAACGTT11.1494252873563218No Hit
CTTTAAAGATTGGCTGTTGGTTCAATCTCTTAGTTCCGTTCGGGATTAAGA11.1494252873563218No Hit
CTTCAAAAGAAAATAATCCTGTTACCCCCAAAGTATCTATCCTGTCTCTTA11.1494252873563218No Hit
GTGGAAGGATTTGTTTTGTAATGAGACGAGGGTCTTGCTGGGTCTAACAGC11.1494252873563218No Hit
ATTAAGAACAGCACCATCTTCAGATCGGCGTTGGATATTAACCAAACTGTA11.1494252873563218No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT11.1494252873563218No Hit
GAGTACGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT11.1494252873563218No Hit
GAGTCTCACTCTGTTGCCCAGGCTGGAGTGCAGTGGTGTAATCTCGGCTCA11.1494252873563218No Hit
GATCTGTCTAGAGTCTTGTATGATTATGAATGAGTGTGGTTCCTGTCTCTT11.1494252873563218No Hit
TCAATCAACTGGTAATCAACACGGTTCACATGTGGAACATCACAATTGTGG11.1494252873563218No Hit
CTCATGCATTATCCAATTGGTCTTAATCCCTTTTTGGAGCTTTCCCTGCGT11.1494252873563218No Hit
GTACCAAAGAAGGATGTCCAACATCATTGATGATGTCCTTGAGCTGTCTCT11.1494252873563218No Hit
GCCAGTTCTTCTTCAATAGCGAGACGAGGAAGTTGACGCTCATCTGCACCT11.1494252873563218No Hit
GTCAAGCTTCGTCACGAACTTAACGTGAAGTCTTCTAGATACATCCTGTCT11.1494252873563218No Hit
GGGTTAGACCGTCGTGAGACAGGTTAGTTTTACGCTACTGATGCCCGCGTC11.1494252873563218No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTCTGTCTCTTAT11.1494252873563218No Hit
AGATAACTAACACATCTCCAAAGTTTTACTAGAAGGGATACCCTCATAGCT11.1494252873563218No Hit
ACTCTGTTTCATTCCTCTGTTTTTTGCTCTGTTTTGCTCTCACGCCATAAA11.1494252873563218No Hit
CATGCTAGAGGTGAAATTCTTGGATTTTGTGAAGACTAACTTCTGCGAAAG11.1494252873563218No Hit
CATCACTATCATGGGAAAACAATAGAAAGGGACTCTACCAGTCCTGATCAT11.1494252873563218No Hit
CCATACATATCGAGGGCTTTATCAATGATTCTTTGGACTGTCTCTTATACA11.1494252873563218No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAG11.1494252873563218No Hit
CTCGGGACAAGCCCACAAGTAAAAACAAAAGAAACGAGAAACCCAAATCCA11.1494252873563218No Hit
GGTGACTGGTGAGAGTTGAGAAGAGGAAGAGTATTACTGTTATTTGGGAAT11.1494252873563218No Hit
GTCTCCGACATGGTGGATGGACTTATCCGTCTTATGGAAGGCAATGATACT11.1494252873563218No Hit
CTAGCAGACTAATCACTAAAAAGGCCTTTGGTTCAAATTGTTACATCTATC11.1494252873563218No Hit
GTTTTAAACGAAACAAAAAAAAACATCATCTTTGTGAAAACTAGAATCTGA11.1494252873563218No Hit
AAACCCCATCTTTGTACTTGATTGCAACAATTGAAGTTCCAGTAACATATG11.1494252873563218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.1494252873563218No Hit
GTCGAACAGACTATTAGTTTGAAAAGCTACCCCCAACTAAAATCAAAAGCC11.1494252873563218No Hit
GTTTATTACCTCGATGTTGGCTTTTGATTTTAGTTGGGGGTAGCTTTTCAA11.1494252873563218No Hit
GAGAGAGAGAGAGAGATGGAACTAGTTTCACCTTCTGTGATACCTGTCTCT11.1494252873563218No Hit
TATTCCTACCATGCTTCTGGATGATGAGAATGGCTGTCTTATCACACCTGT11.1494252873563218No Hit
CCAGCATTCTCTCTTTAGTATTGTCCAAAATGAATTTATTCATTTCTTCAC11.1494252873563218No Hit
AAACAGAACGGATATTCAGAAGGTTATGGCTTTATTGAGTTTGTGAACCTG11.1494252873563218No Hit
ATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCCTGTCTC11.1494252873563218No Hit
GTTTTATTATTGCGAACTTTTTTAGGGTGTATGGATAGATACTTTGGGGGT11.1494252873563218No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA11.1494252873563218No Hit
GGATACCCGTGCGACGTGGCTCGTACATGGTCAAAATTTGATCAATATGTG11.1494252873563218No Hit
GTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTT11.1494252873563218No Hit
TTGCTAAAGTTTGTAATGACATCTTGTCTGTCATTGATAAGCATCTCATTC11.1494252873563218No Hit
GTACGGGACTTCTACGGTAAGTGAAAGAGAGAGAGAGAGAGGAAAAAAAAG11.1494252873563218No Hit
TCACATGTGTGTGTGTGACGTAATCCTAGTACACACACATAACTCGCTGTC11.1494252873563218No Hit
AAAAAGTTCCTCGATGGTCATACAAATTAATAAGTGAGTTGGAAGAATTGG11.1494252873563218No Hit
TGTGTAAAGAGTATGATCTCTTTGGCATTGATGTGATTGATGAGGAAATCA11.1494252873563218No Hit
GAAGGTTTCTCTAGTGATGAAGGGCATATAGGAGTTAGGCGATTTCTTTTG11.1494252873563218No Hit
ATTATTATCCATGCCAAAATACTCAACTAGTGAGCTGTTACGCACTCTTTA11.1494252873563218No Hit
ATGTTAGTATTGTTCAATCGAAGCTTCCAAAGAAGCTCAAGAAAAGAAAGG11.1494252873563218No Hit
CATCGGAAGGTTTCAGACGAAACATCGATTTCTCTATACCTGTCTCTTATA11.1494252873563218No Hit
GAGTGGAAGGAGAGTAAGAGACATAGATTAGATGATGATGATGACAATGAT11.1494252873563218No Hit
CGGTAGTGAGTGGGGTTTGACTTTCGCTTGCTGTCCCCAGATTGATGGAAG11.1494252873563218No Hit
GTTGCTGTCTGTACTCCCAGCACCTAACCCTCTGGAGGAATCTCATCACCT11.1494252873563218No Hit
TTACTCATGCCAGCATTCTCTCTTTAGTATCTGTCTCTTATACACATCTCC11.1494252873563218No Hit
GTCAGACCCACGGGACAATCCGGTCAAATGGAACGAGTTCCTATCGTAGTC11.1494252873563218No Hit
GGATATTTATTGCCTTGTCAGGAGTATCTCTTTCATTTTTTATTCAGGGCT11.1494252873563218No Hit
TGTTCATCCAGGTCTGTCGTCGGTGACGATAGTGCACTCGCTGTCCTGTGC11.1494252873563218No Hit
GGATCCCGCCAATCAGCTTCCTTGCGCCTTACGGGTTTACTCACCCGTTGA11.1494252873563218No Hit
CACTTGATCAAGAAGTGATTCAAGAAGAGACAGTGTCTAACTTACACGTGG11.1494252873563218No Hit
ATTTATTCCTTTTCCTGCATTCTTTAATACCTAGTCTGTCTCTTATACACA11.1494252873563218No Hit
GTTGAATATCTTCCAAAGTTGCAGGCGTCTCAGCAATGATTTGAATCGTTC11.1494252873563218No Hit
TGTTCACAGTCCACTGTCCCGCTGCCAAGAGGCTGATCCTGGCAGAAGCAC11.1494252873563218No Hit
GAATAATTTTCATGTCATGACTCCCGTACTCTGCGTTGATACCCTGTCTCT11.1494252873563218No Hit
TCGCTTAACAGTAAGGTTGTTAGTCAGTACTCTACATTGCTTGCTCCTTTT11.1494252873563218No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTG50.045.037
TTTGAGA50.045.040
CGACAAG50.045.021
GGAGAGA50.045.027
ATCTTTG50.045.033
GTTTGAG50.045.039
AGCTCAA50.045.05
TCGACAA50.045.020
AAATCGA50.045.017
TAAAATC50.045.015
GATGTAA50.045.045
TGTTTGA50.045.038
TAGCTCA50.045.04
TTGAGAT50.045.041
TCAAAGT50.045.08
ATAGCTC50.045.03
AATAGCT50.045.02
GATCTTT50.045.032
GACAAGG50.045.022
AGAGATC50.045.029
Produced by FastQC (version 0.11.2)