FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_74.3410000000aa9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_74.3410000000aa9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAAAGCTCATGAAGGACACCTCGGTCTCTTGTGATCAAGTAATCCATTT10.9523809523809524No Hit
ACTCAACACCAGAGATCTTGGTTTAGTGTTATAATGGAAGGTGTTGGACTA10.9523809523809524No Hit
ACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG10.9523809523809524No Hit
GTTCTTAGCGACACCGTGTTTATTCAGGATCTTTAGATCTGGTCTAAAATC10.9523809523809524No Hit
GCTATATACAGAGGGTTTTCCGTTCTGGACATCTTCATCCCCAGCCTCGGA10.9523809523809524No Hit
GGCAAATATAGGTTCGATCAAGATTTGTTTTTCGGGAGTACCAAAAGCAAG10.9523809523809524No Hit
GTATTGAATGTCACCAGAGAAAGAAAAGAACTCTTTGACATCTTTCTTAGA10.9523809523809524No Hit
AAAGAACAGAGGAGATAGGAAAAATCACTTGGTGATGACTGTCTCTTATAC10.9523809523809524No Hit
CACTTACTCTTGCGTCGGAGTATGGCAACACGACCGTGTTGAGATCATTGC10.9523809523809524No Hit
CATAAAAATAAAGTCAAATCAATTAAACCTTGACCTGACACTTCATCTGGT10.9523809523809524No Hit
CCCTTACCTCTATCAAAGGTATTGGAAGGAGATTGGCCAACATTGTCTGCA10.9523809523809524No Hit
AACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCA10.9523809523809524No Hit
AATGAGAACTTCCGTCTTCTCTATGATACCAAGGGACGTTTCCGTCTCCAC10.9523809523809524No Hit
TGCCTGAACACATAGGGCAGTTGGAGGGAACGTGGGGAAGTGAAACATCTC10.9523809523809524No Hit
CAACTGGAATGGAATGGAAGGGAATGGAATGGAATGGAATTGAACCAAATG10.9523809523809524No Hit
ATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGCGCGAACTTCG10.9523809523809524No Hit
TGTGATGTATGATATTACCCATTACTATCTACACCATGGTCAACCTAAAGA10.9523809523809524No Hit
ATGAAGAATTGAGCAAATTGCTTGGAGATGTGACTATTGCTAATGGAGGTG10.9523809523809524No Hit
ATTCCCCACTCCGTATGCATAATTGCACAGCTTGGTCAATATTTTTAGACA10.9523809523809524No Hit
AACAGGAGCCGGAGATTTTGGTGACTCAACCGGTGAAGAAGCCGAAGCAGT10.9523809523809524No Hit
AGGTAGGGTCCAGCGGCTGGAAGAGCACCGCACGTCGCGTGGTGTCCGGTG10.9523809523809524No Hit
CATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTTCTA10.9523809523809524No Hit
ATCTATTATTTTCTTAGCATCTTTTTTAGCATCTTTTCCATGTCCATAGAA10.9523809523809524No Hit
ACAGTTCACAGCGCCCTTTAGGAATCTTCAGTCAAGGTACGAAGTAACTCG10.9523809523809524No Hit
NAGGGTGCGCGTGCGGACCTGACCAAGGCCCTCAGCATGAACCCCATTTTT10.9523809523809524No Hit
GGCTTTACATGTATGTTGTCCCAAATAAGTGGTTCTGTAAACTGGAGGACC10.9523809523809524No Hit
TCTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGTC10.9523809523809524No Hit
TATCAACGCAGAGTACGGGACTTCTCTCTCTTAGGTTTTTTTTCCCTTCTC10.9523809523809524No Hit
ATAGTATATCTAGCGATAGTGAAGAGTAACGTGAGTGAAAGGTGAAAAGTC10.9523809523809524No Hit
ACTTAGATCCACATATAACGAGCAAGTAAACACATTCCCCAATCTGTCTCT10.9523809523809524No Hit
GGTATTCATTACGACAGTCACCATGTTTTCGTCTACGGGATTCTCACCCTC10.9523809523809524No Hit
GTGGTGATGTAGTCCCCAAGGATGTGAACGCAGCGGTTGGCACCATCAAGA10.9523809523809524No Hit
CTCTCAAACTAATTTGTAACCAAGTGAACAAAAAAATCGTCAGATTATGAG10.9523809523809524No Hit
GAAAAGAGCCGTGGCAGCCATGGACTGAATCAGATGAGAATTCTCTGCTCC10.9523809523809524No Hit
CTCTTTAGTTGATTACAAAGACAAATCAAAACCAAACCTTAAACTGACTTC10.9523809523809524No Hit
CCACAAGCAAGACCGGAAGAGAGATGAGCATAGCCATCGAATAGATAGTAA10.9523809523809524No Hit
GTGCTCCATATCTAAGGAAGATAGATCTAACGATGTATAAACAACTGTCTC10.9523809523809524No Hit
GTTGTTATTGTGGCTTTCGTTGATTGCATTTGATCCTTTATTTTGTAATTT10.9523809523809524No Hit
GTGATATTTAAACCAAATGCAAAAACCATAACCGGACAATGAGAAAGTACT10.9523809523809524No Hit
AGGTAGATTTGATCAATATCGATTCTGTTGTGAGAGAAAACAAGCTATTAC10.9523809523809524No Hit
ATCTTCCCCACCGCGCCAAACAACATAGCAACAATTGCTCCATCCTCTTCC10.9523809523809524No Hit
CTATCGGGTAAAGCCAATGATTAGAGGCATTGGGGGCGCAACGCCCTCGAC10.9523809523809524No Hit
TAGCACAACCGCCGTAATCAGCACCGTGAATCTCGCCATTTTTTTATCTCT10.9523809523809524No Hit
GTGTGCTACGCCGATTGCGATAGACCCACTTGCAAATCCCAATGCCGCATG10.9523809523809524No Hit
GCTCGAGAAGAACATCGGCGGCATCCAGGACATGACCGCGCTGCCCGACGC10.9523809523809524No Hit
GTAAAGTATTGACCATAGTTACACGTGAAGTATAAGTGATGGAGCTGTCTC10.9523809523809524No Hit
GTCAAGGATGTTGTGGAGGATCCACAAAGGCTTCGTCTTGACATATAAAAG10.9523809523809524No Hit
ATGTCCATGGCTTTGTTTTGATTAGCCAATGTGTAATTTCATCATCAATGC10.9523809523809524No Hit
ATTGTTTATTTTGCTTCAGTTGACACTTTATCCGAAATCATATGCCTGTCT10.9523809523809524No Hit
GCTGCATTCATAGCATTTGGGAGATCCTGCTTGTTAGCAAATACGAGCAGA10.9523809523809524No Hit
CTGATGGACAAATCTGTTTGGAAACAGAGCTCTTTTATCGCGGAATTAGAC10.9523809523809524No Hit
GGACACGATAGCTTGGATGCAAGAAGGTGGTCAAGTTGGAATCTTTGATGC10.9523809523809524No Hit
GAAGGACCAAGTCCGACGTATCTGCCAAAGATTCACTGATGCAACTAAGAA10.9523809523809524No Hit
CTTTATGAGTAATAATGGTGTCTTTTGCTTTCGGTTGTTCTTCCTCTTACC10.9523809523809524No Hit
CTCTTATTCAACTCAAAGCTCCCATTATGAGGGTGTTAATGATAAGATCCT10.9523809523809524No Hit
ATCAAGCAAGTTGGGGAACAAAATCTTCCTTGTTAGTTACTCATTTCTTCC10.9523809523809524No Hit
GTATCTCCCCATGGCCTTTCGCCTCTGAAAGCGTCCTTCCTTCTCAATGCC10.9523809523809524No Hit
AACGAAATTAGAGCATCTTCTACAGCAATCTGAGACTTACTTGTATATCTA10.9523809523809524No Hit
TTATTACGGTGGTGAAGTATTTGGATACCTAGACATTGCATTAATGGGATA10.9523809523809524No Hit
GAATTAACAAACAACGATTCACAGTTTGTGTTTGTTTCTGATCTTCGGAAG10.9523809523809524No Hit
CCGTTAGAGTGAGTGCGCTTGACTACTCCTTCGACTGATTGTTCGCATCGG10.9523809523809524No Hit
TATCAACGCAGAGTACGGGGCGTGCCCTAGTTATTCAGATCGCCGAATTTG10.9523809523809524No Hit
CTTAAGCGCCATCCATTTTCGGGGGCTAGTTGATTCGGCAGGTGAGTTGTT10.9523809523809524No Hit
AACTATGGCTGAGGAGTACAAGAACAACGTTCCCGAGCACGAGACACCAAC10.9523809523809524No Hit
GTTCATACACCTTTTACAGGATCATTTAACACAAGCTGTCTCTTATACACA10.9523809523809524No Hit
GCTGAAATGTGCGGCTTGTGGGTTTCTCTCGGATGTTGATATGAGGGACAA10.9523809523809524No Hit
GAGTGAGAGTGAGAAGAGCGGATTCATCAGCCTGGTTTCACGTTACCTGAG10.9523809523809524No Hit
ATTGATTAGAAGATGACAAGTCCACAAGTGAAGACCGGTTTGTTCGTGGGG10.9523809523809524No Hit
ATATCAAACGATCCAATTCGGGTTCAGTTTAGAAACCGTGGATCTTGATCT10.9523809523809524No Hit
CGTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAA10.9523809523809524No Hit
GTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGG10.9523809523809524No Hit
CTTCAATGTAGCTTATATTGCATCAATTCCAAATTTGTTCCATATTGTTGA10.9523809523809524No Hit
CGGTCACTGTTACTAACGATGGTGCTACTATTCTCAAGTCACTTCACATAG10.9523809523809524No Hit
GGATTGAGACATTTTGTGATTTGTTGCTGCTTTGGAAAGTTTGGTTTGTTG10.9523809523809524No Hit
GTCATAGACAGACTGGACAACAAGACCATATGAGAACGGCCTGATGCCAAG10.9523809523809524No Hit
AACTGGTAGCCTTTACTCTGACTGGGATCTTCTCCCACCCAAGAAGATCAA10.9523809523809524No Hit
CTTGCAATCGGTTGAGCGCCGGTTGAAAGCTCTTCAGCAGACTGGATGCAG10.9523809523809524No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.9523809523809524No Hit
CCATATTTCTCGCCAAGGGCTTTGTGTGCATCAGCATCCAAATTGGCGATG10.9523809523809524No Hit
ATCAACAGCGGTGTGGAGAAGAGAGCAGGAGGTTGAGACCACAAGCTTGTC10.9523809523809524No Hit
GTTAAAAATCCAGTTGTTTGTTGTACAAAGCTTTAAGACTGTCTCTTATAC10.9523809523809524No Hit
CCTGTAATCTTGCTGACTTCTCTGATACTTTTGAACATACCTGGACCTGTC10.9523809523809524No Hit
ACGATAGAATGTAAAAAGAGCAATTTGATTTCGAAAATTGAATTTTAGTCC10.9523809523809524No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA10.9523809523809524No Hit
GGTAGAGATACCAGCATAGTCATGGAAATACTGAACCTGTCTCTTATTACA10.9523809523809524No Hit
TCAGAGTTCTACTCAGAAGACAAGACCTACGACTTGAACTTCAAAGAAGAG10.9523809523809524No Hit
GAACCAAGACCGTGGAAGCAATCGAAGAATCTATCGTCTCTGTTTCACCAC10.9523809523809524No Hit
GAGACAAGAATAAGGAAATTATTTGTTCGATATTGATAGAGCTCAAGCTCT10.9523809523809524No Hit
AGTCAAGCTGCTGCAAGACACCAGAGCAGAAAGTGGCGGTTTTTCCAGTAC10.9523809523809524No Hit
ACCCACAGCTCATCCCCTAATTTTTCAACATTAGTGGGTTCGGACCTCCAG10.9523809523809524No Hit
TCATGATATTGTCTCTTGGTAGAAAATGAGAATAGCAAAAGCTTTGATCTT10.9523809523809524No Hit
GTAAACAACGTGGCCATAACAGAAAACGTTTACGATTTGACAGTTGAGGAG10.9523809523809524No Hit
GCTCTCTATTCTGGCTGTTGACTACCCAGTTGACAAGGTGTCCTGTTATGT10.9523809523809524No Hit
GTCGGTAAGTTCATGCCGAAATTCAAATCCCTTTCTCCGGCGAACAAGACT10.9523809523809524No Hit
CTTCTTATATGATCTTTCACCTATCACTGTGACCATCAAGGAAGAACGCCG10.9523809523809524No Hit
CCTCGACAGCTGCAACTGTTGAAAATGCCCAACAGCTACCACATTGACCCT10.9523809523809524No Hit
CCTTATTAATCATCTCAAAGAAAACTCTTACTGGAGACTTTGGCGGCTTCA10.9523809523809524No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC10.9523809523809524TruSeq Adapter, Index 12 (95% over 21bp)
GCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTC10.9523809523809524No Hit
CCATTGAGTCTGTTAAGCTTCGGATTCAGTCTTACCGCGGCTTTGTTGTGA10.9523809523809524No Hit
AATTTAGACCCTTTGTGTGTGTTTCTTGTTGCAAGACAACCATATCTATTG10.9523809523809524No Hit
TGAAAATCCGGAGGACCGAGTGCCGCTCACGCCCGGTTGTACTCATAACCG10.9523809523809524No Hit
GTAGTGATTCTGATCCCAAACCCGAATCCAGTCCTCTCTGCATCAGATGAA10.9523809523809524No Hit
GGGTAAGAGTCTGGAGGAGGCATGACGCAATTGTCTCCATTAAAGTAAATC10.9523809523809524No Hit
GGTATCAACGCAGAGTACGGGGACCTTTTAAATGGGAAAGCCAACGGCGAA10.9523809523809524No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers