FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_73.3410000000aa71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_73.3410000000aa71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTGTTGTGTTACAATTCTTTTCTTTTTGGGTTTGTGACGTGAGATTTG12.1739130434782608No Hit
CGTACAAGTACCGTGAGGGAAAGGTGAAAAGAACCCTATGACGGGAGTGCA12.1739130434782608No Hit
CTATCCTCTTCCCTATGTATCCAGTCGCTCCAACCACCAAAACGCGCGTTT12.1739130434782608No Hit
GCAGACATTCTAGAAGGATGATTTACTCAAAGATATCATCATCGTCATCGG12.1739130434782608No Hit
CAGTAAGGTCACGTCCAGCAAGGTCAAGACGGAGGATGGCATGAGGAAGAG12.1739130434782608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT12.1739130434782608No Hit
GTAAACTACTGAGTGATGAGATTGATTCTAAGTTTGAACATAAAAACCAGT12.1739130434782608No Hit
AAGATACACAGACCGAACTGCTCTGACTTCTCTGACTAGTTAACAACATAT12.1739130434782608No Hit
ACGCAGAGTACGGGGAGATCCCCAAAAGACAGAGAACTTTCTCGATACGAA12.1739130434782608No Hit
GACGTATTGAACTCTACCGGATCTAATTGCTTCACCGAGAATTCCAGTTTC12.1739130434782608No Hit
GCCAGTGATATACTGGAGGAAGGTGGGGATGACGTCAAGTCCGCATGGCCC12.1739130434782608No Hit
GACGGCAGCCCTTTCGGGGGTTCCTTAGGGACCGATTCACTCTGCGTAGAT12.1739130434782608No Hit
CTATTAAACCAGCAGCCTCGTCGCCCTGGGTGCTCTCTTTCCCTACTTGCT12.1739130434782608No Hit
ATGGAGAGAGATGGGAAGCATGAACAATGGTAACATCCTAATTCACTTTAC12.1739130434782608No Hit
GTTCGGCGACATTGGTGACCGTGTCGGCGATGCGGAAGTACTGTGGAGCAA12.1739130434782608No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCGAGACCCCGTTAGTAGCGG12.1739130434782608No Hit
ATCTTAGTCAAAGGGATACATGTTCGCGTAATTGCATTGGTGTGGTGATCA12.1739130434782608No Hit
CTTTGTTTCCAAGACAATGGAGGAAGATCAGCTCCATATAGATGACCTGTC12.1739130434782608No Hit
TGATCGGTGCAGGAATCTTGACCTGCTGTCCATCGGCTGCGCCGTTCGGCC12.1739130434782608No Hit
TTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGT12.1739130434782608No Hit
GGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAA12.1739130434782608No Hit
GGGTATCTGTTTTCAAGAATACTCGGAATTGATGTGGCATCATCGAGAACT12.1739130434782608No Hit
GTGTAACCAGCAATCTTGTTTCTCAATCTCTTGGATTGGATAGTGGCGATT12.1739130434782608No Hit
GTATTAGGAGGTGGGATATTTAAGAAGTGATTTGATCATAAAAGCTCTGCC12.1739130434782608No Hit
TTCCTGTTGTGGTCCTGATAAGTCCAACATCAAGAAGAGCACGGAAAGGTC12.1739130434782608No Hit
GGAGGCGATGAGCATGTACCCAAAGTAAAGCATCGCAGAGACCAGTTTGGT12.1739130434782608No Hit
TATTGTTGGGAGGTCCGTTATGGAAAGAGCCTGTAGAGATCCTGAAGATGA12.1739130434782608No Hit
ATGGTAGAATGGCCGAAGGAGGCGAAGTGGAAATGGAAGTTATTCCGATGG12.1739130434782608No Hit
TCACAGAAGTGCTGAACAGAGGAAAGTCATCAGGCAAGCATACCACCAAAC12.1739130434782608No Hit
TTACTCGTCCATGCGTAGGCGCATCCAGTCCGGCCATGTCGCGCATCAGCG12.1739130434782608No Hit
TCATAAGACTTACGAAGAGGAAGAGGAAGGTACAGTTGAAGGGTTGAAAGG12.1739130434782608No Hit
GTAGAAGAGTGTGTAAGTTGTGAGTCTCTATGTCTGTGCAGATGTGTGTTT12.1739130434782608No Hit
GATTGGAATAGAATGGAAAAGAATGGAATGGAATAGAAAAGGAATGGAATG12.1739130434782608No Hit
GAAATGGGAAGAGCGGCTTAAGCCATTAGGTGTAGGCGCTTTCCAAAGGTG12.1739130434782608No Hit
CAATTAGGATTGTATTTCGTCGAGGTATAGTTTCTGTCTCTTATACACATC12.1739130434782608No Hit
CGCATGACCGGCTCGCCGTCGATCTCCAGCTCCAGAGTGACGAACTGCCCC12.1739130434782608No Hit
CTACATATCAGGCCAATTATGGCTTCTCTAACGATCTGTCTCTTATACACC12.1739130434782608No Hit
CGGCGAGCGAGAGCGGATTTGGGATTTTAAGAAAAAGAAAGACGAAGCTTC12.1739130434782608No Hit
CCTCTATGGCGTCCCGTTCCAGGGAACTCGGAAAGTACCTTGCCAAAAGCA12.1739130434782608No Hit
TCGCTCATGCAGGCATCCTTGTCACTTGTTCAGGGAGGTAGTGCGGGCTTG12.1739130434782608No Hit
GTAACAAAGACATCATCATGGTCGACCACATGAGGAAGATGAAGAACAACG12.1739130434782608No Hit
CTCCTGAAGATTTTAATGGATATTACAAGAGAAAAACATTTCTGAGGCCCA12.1739130434782608No Hit
AAACTTCTCTAGCCAACAAGTACCAGATTAAACCGATGGAGTGCTTACCTC12.1739130434782608No Hit
CAGGGAAACTCCGAATACAGGCCGTTCTCGTTTGTACAGACAGACTTTTGG12.1739130434782608No Hit
GGGTGAGAGTGAGTGGGTAGGTACTGTAGGAGAAGAAGAAGAAGAAAAGAG12.1739130434782608No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAAGGGTGTTGGTCGATTA12.1739130434782608No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)