##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_3_70.3410000000aa4b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4 Sequences flagged as poor quality 0 Sequence length 51 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.0 0.0 0.0 0.0 0.0 0.0 2 36.5 0.0 0.0 0.0 0.0 0.0 3 38.0 0.0 0.0 0.0 0.0 0.0 4 38.0 0.0 0.0 0.0 0.0 0.0 5 35.0 0.0 0.0 0.0 0.0 0.0 6 39.5 0.0 0.0 0.0 0.0 0.0 7 40.0 0.0 0.0 0.0 0.0 0.0 8 40.0 0.0 0.0 0.0 0.0 0.0 9 40.0 0.0 0.0 0.0 0.0 0.0 10 39.5 0.0 0.0 0.0 0.0 0.0 11 39.0 0.0 0.0 0.0 0.0 0.0 12 39.5 0.0 0.0 0.0 0.0 0.0 13 39.0 0.0 0.0 0.0 0.0 0.0 14 39.5 0.0 0.0 0.0 0.0 0.0 15 38.0 0.0 0.0 0.0 0.0 0.0 16 39.0 0.0 0.0 0.0 0.0 0.0 17 39.0 0.0 0.0 0.0 0.0 0.0 18 39.5 0.0 0.0 0.0 0.0 0.0 19 39.5 0.0 0.0 0.0 0.0 0.0 20 39.0 0.0 0.0 0.0 0.0 0.0 21 38.0 0.0 0.0 0.0 0.0 0.0 22 39.0 0.0 0.0 0.0 0.0 0.0 23 39.5 0.0 0.0 0.0 0.0 0.0 24 39.5 0.0 0.0 0.0 0.0 0.0 25 40.0 0.0 0.0 0.0 0.0 0.0 26 40.0 0.0 0.0 0.0 0.0 0.0 27 39.5 0.0 0.0 0.0 0.0 0.0 28 39.0 0.0 0.0 0.0 0.0 0.0 29 40.0 0.0 0.0 0.0 0.0 0.0 30 39.0 0.0 0.0 0.0 0.0 0.0 31 40.0 0.0 0.0 0.0 0.0 0.0 32 39.5 0.0 0.0 0.0 0.0 0.0 33 39.5 0.0 0.0 0.0 0.0 0.0 34 40.0 0.0 0.0 0.0 0.0 0.0 35 39.0 0.0 0.0 0.0 0.0 0.0 36 39.5 0.0 0.0 0.0 0.0 0.0 37 40.0 0.0 0.0 0.0 0.0 0.0 38 39.5 0.0 0.0 0.0 0.0 0.0 39 40.0 0.0 0.0 0.0 0.0 0.0 40 39.5 0.0 0.0 0.0 0.0 0.0 41 32.75 0.0 0.0 0.0 0.0 0.0 42 36.25 0.0 0.0 0.0 0.0 0.0 43 38.5 0.0 0.0 0.0 0.0 0.0 44 32.75 0.0 0.0 0.0 0.0 0.0 45 37.5 0.0 0.0 0.0 0.0 0.0 46 38.5 0.0 0.0 0.0 0.0 0.0 47 37.5 0.0 0.0 0.0 0.0 0.0 48 32.25 0.0 0.0 0.0 0.0 0.0 49 36.25 0.0 0.0 0.0 0.0 0.0 50 35.75 0.0 0.0 0.0 0.0 0.0 51 30.75 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 36 1.0 37 0.0 38 1.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.0 50.0 0.0 25.0 2 0.0 25.0 50.0 25.0 3 25.0 75.0 0.0 0.0 4 0.0 25.0 25.0 50.0 5 50.0 25.0 25.0 0.0 6 50.0 25.0 25.0 0.0 7 25.0 25.0 25.0 25.0 8 25.0 25.0 0.0 50.0 9 50.0 0.0 25.0 25.0 10 50.0 25.0 25.0 0.0 11 50.0 25.0 25.0 0.0 12 25.0 25.0 50.0 0.0 13 50.0 50.0 0.0 0.0 14 0.0 0.0 75.0 25.0 15 50.0 25.0 0.0 25.0 16 25.0 50.0 25.0 0.0 17 0.0 50.0 25.0 25.0 18 25.0 50.0 0.0 25.0 19 50.0 50.0 0.0 0.0 20 0.0 25.0 25.0 50.0 21 50.0 25.0 0.0 25.0 22 50.0 25.0 0.0 25.0 23 0.0 75.0 0.0 25.0 24 0.0 75.0 0.0 25.0 25 0.0 75.0 25.0 0.0 26 0.0 75.0 25.0 0.0 27 0.0 50.0 50.0 0.0 28 0.0 75.0 0.0 25.0 29 25.0 75.0 0.0 0.0 30 0.0 100.0 0.0 0.0 31 25.0 75.0 0.0 0.0 32 25.0 50.0 0.0 25.0 33 0.0 25.0 75.0 0.0 34 50.0 25.0 25.0 0.0 35 0.0 50.0 50.0 0.0 36 0.0 25.0 25.0 50.0 37 0.0 75.0 25.0 0.0 38 0.0 50.0 0.0 50.0 39 25.0 50.0 25.0 0.0 40 50.0 0.0 50.0 0.0 41 0.0 75.0 25.0 0.0 42 0.0 25.0 25.0 50.0 43 0.0 50.0 0.0 50.0 44 25.0 0.0 50.0 25.0 45 25.0 50.0 0.0 25.0 46 25.0 0.0 0.0 75.0 47 25.0 25.0 25.0 25.0 48 50.0 0.0 50.0 0.0 49 0.0 0.0 50.0 50.0 50 25.0 25.0 25.0 25.0 51 25.0 25.0 25.0 25.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.5 33 1.0 34 0.5 35 0.0 36 0.5 37 1.0 38 1.0 39 1.0 40 0.5 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.5 49 1.0 50 0.5 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 4.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACGGGGTGGTATCAACGCAAAAAAAAAAACTGTCTCTTATACACATCTC 1 25.0 No Hit ATACAGTCCATTATGGTGGAGGACAATCAAGGTGACAAGGACCTGGGGTAG 1 25.0 No Hit CAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAGTACTCCTGTCT 1 25.0 No Hit ACATTACAGTAAGCGAAAATCGCATTTAGAAATTTTACATACAGACCTCGA 1 25.0 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 25.0 33 0.0 0.0 25.0 34 0.0 0.0 25.0 35 0.0 0.0 25.0 36 0.0 0.0 25.0 37 0.0 0.0 25.0 38 0.0 0.0 25.0 39 0.0 0.0 25.0 >>END_MODULE >>Kmer Content pass >>END_MODULE