FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_63.3410000000a9df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_63.3410000000a9df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAGGACAAGTACCCCTGGAATTTTTGCTATTGGAGATGTCGCAGCCTT11.4084507042253522No Hit
ATGATCTCCGACAGTGATTACACCGAGTTTGATAACTTCACCAAATGGCTC11.4084507042253522No Hit
TCTTTCTTCCATCCCATCTGGTATCTTCTTCTTCCACTCTTCCATCTGTCT11.4084507042253522No Hit
ACTCTATGCTTGTGATTTTGAATCAAGACTTATACATTGAGCAGTTTAAGC11.4084507042253522No Hit
CTTTTGGTATGTGATATAAGCCAATTATCAAGTCAGCACTCTTTATGCTGT11.4084507042253522No Hit
GACTCTATGAGGGAAACAAAACGATTGATGGATACAACAAGCTGTCTCTTA11.4084507042253522No Hit
GTAGATCTCTCAGATACCATCGGATCTTCTTCATCTTCCGATCTTTTACTC11.4084507042253522No Hit
GTCTCCTCCTTTGCCATCGTTAAGAACTGGCTCTGATTGACACAGAAGTAA11.4084507042253522No Hit
GTCAAGAAAAGCCACTATAGTATTATATAAGCCCGTACTCTGCGTTGATAC11.4084507042253522No Hit
CATCATGATTCTCTGTTTTAACCAATATCAAGCATTTTTAAGCTGTCTCTT11.4084507042253522No Hit
CTCTTGAAAAAGTGAGAGCTTTAACTTGGGTTTTCACATTTTCTCTCTTTT11.4084507042253522No Hit
GAATGAGATCGGAGAGGCGATATATCCATTAAAGAGGAGATTTTGTGTTTG11.4084507042253522No Hit
GCATTAAGAGCCCCAATAACAATGTCGACTTCATTCTTGTTTATCTCGGCC11.4084507042253522No Hit
ATAGAGAGCTTTCTCCACAACTATCACAGAGACTGTCTCTTATACACATCT11.4084507042253522No Hit
GGTTTTCACCTTCTTCCTCATCCTCGTAGTCACCTTCTTCATCAGCCGTTG11.4084507042253522No Hit
GAGGCAGAGAAGAAAGATGAAGAAACCGAGAAGAAAACAGAGGAGAAAGAC11.4084507042253522No Hit
ACTTGTTATGAAATCGATTGTTCCTGTTGTCATGGCTGGTGTGTTAGGTAT11.4084507042253522No Hit
GGGGACAAGACATTCATAGAGTCTATATAAGTATCCTAACAATTCAAGTAA11.4084507042253522No Hit
ACTTCAAGATTGACGGTGTTTACAGAAGTGGAAGCATACAGTACCTGTCTC11.4084507042253522No Hit
GACTTGATCAATGTTGGTAGAGTTCTTGCACTGTCTCTTATACACATCTCC11.4084507042253522No Hit
GTAATCAATCATATGGTTAATAAGCAAAAAAACAAATACGGCCAAGCAGAG11.4084507042253522No Hit
GTAGAAAGTGATTTCCCTAATGAAAAAGCTTTCAACGATGTCCAATATCTG11.4084507042253522No Hit
GATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAAC11.4084507042253522No Hit
GTTTCTAAACTGAACCCGAATTGGATCGTTTGATATATCTATACCTGTCTC11.4084507042253522No Hit
CGTTACCACCGTTTAGGACCAATGAGGTCATGATTCCTGTTGAATGAATTT11.4084507042253522No Hit
TGCTAACAGATCCGGAGGAAGAGGGAGATGTTTCTACGGCTGTCTCTTATA11.4084507042253522No Hit
AGAGATAAAACTGTTGTTCTCTGTTTCCTTTGCCTTGAGCAAATACATTAG11.4084507042253522No Hit
ACAAAAACCTAGATAGTAGAGAAGGAGCCGATATAAGACCCTGTCTCTTAT11.4084507042253522No Hit
AGTCAGACTAAAGTGAGGGATGATGATGTAGCAGAACACAAAGCTGTCTCT11.4084507042253522No Hit
TGCACAATCAGTTGGAACAAAGTCTATTGAGGATGAAGCTTGGGATCTGTT11.4084507042253522No Hit
GGATATCTCTGGAAACCGCAAAGCTGTTGTGATTTACGTTCCATTCAGATT11.4084507042253522No Hit
GTCCCCAATTCGGAGCTCATCTAATTCTGCACAAGCTCTGATTTTGTATAT11.4084507042253522No Hit
AAACTACATAGGATACTGTTTTTGGACGATGATGTGGTTGTGCAGAAGGAT11.4084507042253522No Hit
CGTAATAGGGTCCTTCGATCTCTTTCCCTAGCATTCTTGATAGACTCATCA11.4084507042253522No Hit
ATATAGGACACAAGACCCATTTTTTCAGTCTAATTAGACCTGTCTCTTATA11.4084507042253522No Hit
ATCCCATTCTTTTCAATTCCATTTCATTCGACTCCATTATATCTGTCTCTT11.4084507042253522No Hit
CAATCGGGCGGTAAATTCCGTCCAAGGCTAAATCTGTCTCTTATACACATC11.4084507042253522No Hit
CTTCTTCTCCATTTTCTTAAAGCTAAAACCTCTCAATCAGGTGATCTCTGT11.4084507042253522No Hit
GTTTATGCAAGTCCTGAAACGTGAATTAGACAGGGTGGAGAAAGAAAAGGA11.4084507042253522No Hit
GTCCCAGGAACAGCAACATTTATAAGCTTACACAAAAGAACACCATCTTTA11.4084507042253522No Hit
AGCTGATCTTTATCAACGAAGGACAAAATCCCAACGTAGTACTCCTGTCTC11.4084507042253522No Hit
ATTCCAAATTGAAAAACAAAACACAAACCAGCTTTGTTTAACGGTAACTCC11.4084507042253522No Hit
GGCTTATCAGGACGAACATACAAGTTAGTGAATCAGCTCTTGGAACACACA11.4084507042253522No Hit
GGGGATAGGATTTGATTTGAAATCAAAGATGGCGTTGAACTGCCTCTGTCT11.4084507042253522No Hit
CAACAAAAAAAGCCAACAAACTTACACATCACAAAAGCAAAAGATGATCCT11.4084507042253522No Hit
GTCTTGAAACACGGACCAAGGAGTCTGACATGAGTGAGAGTCAACGGGTGA11.4084507042253522No Hit
GTTTTTAAATCTGTGTTATCAGGTGTTGTCTGTCTCTTATACACATCTCCG11.4084507042253522No Hit
CTTCACTACAATTTAGTCCAGAAGACGGATGGCTCTCTTCTTTCTATTCAT11.4084507042253522No Hit
GTACGTGAATGAGTATCATCAGATGTCATAATAATGAAAGGAATGTCTCTC11.4084507042253522No Hit
TTCCAAGTCTGTAAGATTGTGTGCTCTTTAGGAAGAACAGATGGATCCGCC11.4084507042253522No Hit
GCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAGATACC11.4084507042253522No Hit
GAGCTATCATAGGATTGACTGTGGCCACATTTTGTTATCTGCAATTTTTCC11.4084507042253522No Hit
ACCAATTTCTTCTCAGGTAATACTCGTAAGATTTATCAACACAAGCAAAAA11.4084507042253522No Hit
CACTAGATGACTTAATCAAGACTCTATGAACCTGTCTCTTATACACATCTC11.4084507042253522No Hit
AAGATAGAGATGCTTTGGTAGATTTGTTTGGAAAAGTTAGAGATGATTGGA11.4084507042253522No Hit
GCCACATTCATTAGAGACACTTTAGTAAACTCAAAGCATACATTGCACCTC11.4084507042253522No Hit
AATATGACGACTTTTTCTGTTCCGATTGATAACGGAGATTCTATGAAGATC11.4084507042253522No Hit
GTGCTCGTTCTCGTTACCAGAAGAGTTACCGTTAAACAAAGCTGGTTTGTG11.4084507042253522No Hit
ACACAGTTATGAAGAAGCATTCTCAGAATCACGTCTAGGTAATTATCATCT11.4084507042253522No Hit
GGAATATGAAGGGAATTATGTATTCTTATCAGTCTTTTGATGAAGTGAGGT11.4084507042253522No Hit
CATTCATAGACAACACTTCCACTTCTCTTGCTTCACTCAAATTTTCAACAC11.4084507042253522No Hit
CGACTTGATCGTCCACATGTCAAAGGCCTAAGCTAAGAAGCAGATCTCGAA11.4084507042253522No Hit
GTATACACCAGCTATGTTAGCATCTCTCAAGTTCTCTAAATTCACACTTGG11.4084507042253522No Hit
GTTAATTGCACTTTCCTCTTGTTTGGATCTGTTATCCAACTCATTGCTTGT11.4084507042253522No Hit
ATTGAATAGATCAAACTCTGTAACTGTTTTGTTGCTGTCTCTTATACACAT11.4084507042253522No Hit
CCCATGGGCCGAGGCTGGAGCTGACTACGTTGTTGAGCTGTCTCTTATACA11.4084507042253522No Hit
CTACACTACTGTACCCTCCTTTTTCTTCTTCTTCAACTTCATACCTTTCTG11.4084507042253522No Hit
ATCAACGCAGAGTACGGGCTCTTCGCCATCTCTTCTTTTCCCGTGAATCCG11.4084507042253522No Hit
CTTCTGGAGATCTCTTCTGGGAATCGAGCTTCACTGTTAAAGATTTTCCTT11.4084507042253522No Hit
GTGCAGATGAGCGTCAACTTCCTCGTCTCGCTATTGAAGAAGAACTGGCAA11.4084507042253522No Hit
GTAGTCAACGAGTCCACTGAAGAAAGACTTTGAAGATAAGTCTTCGTAGTG11.4084507042253522No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGTC50.045.024
TTGTCTG50.045.026
GTGTTGT50.045.023
AATCTGT50.045.08
GTGTTAT50.045.013
ATCTCCG50.045.045
TAAATCT50.045.06
CACATCT50.045.042
AAATCTG50.045.07
TATACAC50.045.038
TCAGGTG50.045.019
TGTCTGT50.045.027
TGTCTCT50.045.031
CATCTCC50.045.044
TTATCAG50.045.016
CTCTTAT50.045.034
TTTTTAA50.045.02
GTTTTTA50.045.01
GTCTGTC50.045.028
ACATCTC50.045.043
Produced by FastQC (version 0.11.2)