##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_3_62.3410000000a9c2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 40 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.775 0.0 0.0 0.0 0.0 0.0 2 37.425 0.0 0.0 0.0 0.0 0.0 3 38.0 0.0 0.0 0.0 0.0 0.0 4 37.425 0.0 0.0 0.0 0.0 0.0 5 37.4 0.0 0.0 0.0 0.0 0.0 6 39.2 0.0 0.0 0.0 0.0 0.0 7 39.0 0.0 0.0 0.0 0.0 0.0 8 38.425 0.0 0.0 0.0 0.0 0.0 9 38.8 0.0 0.0 0.0 0.0 0.0 10 38.0 0.0 0.0 0.0 0.0 0.0 11 38.95 0.0 0.0 0.0 0.0 0.0 12 38.0 0.0 0.0 0.0 0.0 0.0 13 37.475 0.0 0.0 0.0 0.0 0.0 14 38.275 0.0 0.0 0.0 0.0 0.0 15 38.5 0.0 0.0 0.0 0.0 0.0 16 38.775 0.0 0.0 0.0 0.0 0.0 17 38.75 0.0 0.0 0.0 0.0 0.0 18 38.375 0.0 0.0 0.0 0.0 0.0 19 38.3 0.0 0.0 0.0 0.0 0.0 20 39.2 0.0 0.0 0.0 0.0 0.0 21 37.8 0.0 0.0 0.0 0.0 0.0 22 38.1 0.0 0.0 0.0 0.0 0.0 23 38.15 0.0 0.0 0.0 0.0 0.0 24 38.85 0.0 0.0 0.0 0.0 0.0 25 38.925 0.0 0.0 0.0 0.0 0.0 26 39.5 0.0 0.0 0.0 0.0 0.0 27 39.5 0.0 0.0 0.0 0.0 0.0 28 38.525 0.0 0.0 0.0 0.0 0.0 29 38.175 0.0 0.0 0.0 0.0 0.0 30 39.3 0.0 0.0 0.0 0.0 0.0 31 39.15 0.0 0.0 0.0 0.0 0.0 32 38.1 0.0 0.0 0.0 0.0 0.0 33 39.125 0.0 0.0 0.0 0.0 0.0 34 39.375 0.0 0.0 0.0 0.0 0.0 35 39.35 0.0 0.0 0.0 0.0 0.0 36 39.55 0.0 0.0 0.0 0.0 0.0 37 39.025 0.0 0.0 0.0 0.0 0.0 38 38.725 0.0 0.0 0.0 0.0 0.0 39 38.15 0.0 0.0 0.0 0.0 0.0 40 39.35 0.0 0.0 0.0 0.0 0.0 41 38.0 0.0 0.0 0.0 0.0 0.0 42 38.675 0.0 0.0 0.0 0.0 0.0 43 38.45 0.0 0.0 0.0 0.0 0.0 44 38.775 0.0 0.0 0.0 0.0 0.0 45 38.85 0.0 0.0 0.0 0.0 0.0 46 38.35 0.0 0.0 0.0 0.0 0.0 47 37.65 0.0 0.0 0.0 0.0 0.0 48 38.2 0.0 0.0 0.0 0.0 0.0 49 38.6 0.0 0.0 0.0 0.0 0.0 50 38.4 0.0 0.0 0.0 0.0 0.0 51 38.625 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2216 1 0.0 2216 2 0.0 2216 3 0.0 2216 4 0.0 2216 5 0.0 2216 6 0.6666666666666661 2216 7 0.0 2216 8 0.0 2216 9 0.0 2216 10 0.0 2216 11 0.0 2216 12 0.0 2216 13 0.0 2216 14 -1.333333333333334 2216 15 0.0 2216 16 0.0 2216 17 0.0 2216 18 0.0 2216 19 0.0 2216 20 0.0 2216 21 1.0 2216 22 0.0 2216 23 -0.666666666666667 2216 24 0.0 2216 25 0.0 2216 26 0.0 2216 27 0.0 2216 28 0.0 2216 29 2.666666666666667 2216 30 0.6666666666666661 2216 31 0.6666666666666661 2216 32 0.6666666666666661 2216 33 0.0 2216 34 0.6666666666666661 2216 35 -0.666666666666667 2216 36 0.6666666666666661 2216 37 0.0 2216 38 0.6666666666666661 2216 39 0.0 2216 40 0.6666666666666661 2216 41 0.0 2216 42 2.666666666666667 2216 43 0.6666666666666661 2216 44 0.6666666666666661 2216 45 0.6666666666666661 2216 46 0.0 2216 47 0.0 2216 48 0.6666666666666661 2216 49 -0.666666666666667 2216 50 -0.666666666666667 2216 51 0.6666666666666661 2204 1 0.0 2204 2 0.0 2204 3 0.0 2204 4 0.0 2204 5 0.0 2204 6 0.6666666666666661 2204 7 0.0 2204 8 0.0 2204 9 0.0 2204 10 0.0 2204 11 0.0 2204 12 0.0 2204 13 0.0 2204 14 0.6666666666666661 2204 15 0.0 2204 16 0.0 2204 17 0.0 2204 18 0.0 2204 19 0.0 2204 20 0.0 2204 21 0.0 2204 22 0.0 2204 23 1.333333333333333 2204 24 0.0 2204 25 0.0 2204 26 0.0 2204 27 0.0 2204 28 0.0 2204 29 2.666666666666667 2204 30 0.6666666666666661 2204 31 0.6666666666666661 2204 32 0.6666666666666661 2204 33 0.0 2204 34 0.6666666666666661 2204 35 1.333333333333333 2204 36 0.6666666666666661 2204 37 0.0 2204 38 0.6666666666666661 2204 39 0.0 2204 40 0.6666666666666661 2204 41 0.0 2204 42 2.666666666666667 2204 43 0.6666666666666661 2204 44 0.6666666666666661 2204 45 0.6666666666666661 2204 46 0.0 2204 47 0.0 2204 48 0.6666666666666661 2204 49 1.333333333333333 2204 50 1.333333333333333 2204 51 0.6666666666666661 2105 1 0.0 2105 2 0.0 2105 3 0.0 2105 4 0.0 2105 5 0.0 2105 6 -1.333333333333334 2105 7 0.0 2105 8 0.0 2105 9 0.0 2105 10 0.0 2105 11 0.0 2105 12 0.0 2105 13 0.0 2105 14 0.6666666666666661 2105 15 0.0 2105 16 0.0 2105 17 0.0 2105 18 0.0 2105 19 0.0 2105 20 0.0 2105 21 -1.0 2105 22 0.0 2105 23 -0.666666666666667 2105 24 0.0 2105 25 0.0 2105 26 0.0 2105 27 0.0 2105 28 0.0 2105 29 -5.333333333333333 2105 30 -1.333333333333334 2105 31 -1.333333333333334 2105 32 -1.333333333333334 2105 33 0.0 2105 34 -1.333333333333334 2105 35 -0.666666666666667 2105 36 -1.333333333333334 2105 37 0.0 2105 38 -1.333333333333334 2105 39 0.0 2105 40 -1.333333333333334 2105 41 0.0 2105 42 -5.333333333333333 2105 43 -1.333333333333334 2105 44 -1.333333333333334 2105 45 -1.333333333333334 2105 46 0.0 2105 47 0.0 2105 48 -1.333333333333334 2105 49 -0.666666666666667 2105 50 -0.666666666666667 2105 51 -1.333333333333334 >>END_MODULE >>Per sequence quality scores pass #Quality Count 26 1.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 1.0 33 0.0 34 2.0 35 1.0 36 1.0 37 0.0 38 7.0 39 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.5 22.5 17.5 27.500000000000004 2 5.0 22.5 50.0 22.5 3 25.0 22.5 30.0 22.5 4 12.5 25.0 32.5 30.0 5 10.0 37.5 42.5 10.0 6 7.5 47.5 20.0 25.0 7 42.5 25.0 20.0 12.5 8 7.5 35.0 40.0 17.5 9 22.5 15.0 17.5 45.0 10 22.5 22.5 32.5 22.5 11 15.0 35.0 35.0 15.0 12 22.5 20.0 35.0 22.5 13 25.0 15.0 37.5 22.5 14 10.0 32.5 32.5 25.0 15 17.5 35.0 30.0 17.5 16 30.0 20.0 32.5 17.5 17 17.5 27.500000000000004 42.5 12.5 18 27.500000000000004 27.500000000000004 30.0 15.0 19 17.5 35.0 27.500000000000004 20.0 20 20.0 15.0 42.5 22.5 21 20.0 20.0 27.500000000000004 32.5 22 17.5 45.0 25.0 12.5 23 7.5 25.0 30.0 37.5 24 12.5 27.500000000000004 30.0 30.0 25 12.5 35.0 37.5 15.0 26 22.5 20.0 17.5 40.0 27 7.5 37.5 40.0 15.0 28 17.5 32.5 30.0 20.0 29 20.0 30.0 25.0 25.0 30 22.5 32.5 27.500000000000004 17.5 31 10.0 27.500000000000004 35.0 27.500000000000004 32 7.5 27.500000000000004 25.0 40.0 33 30.0 30.0 12.5 27.500000000000004 34 20.0 35.0 30.0 15.0 35 25.0 30.0 27.500000000000004 17.5 36 30.0 25.0 27.500000000000004 17.5 37 25.0 32.5 20.0 22.5 38 15.0 30.0 30.0 25.0 39 20.0 30.0 22.5 27.500000000000004 40 7.5 37.5 30.0 25.0 41 20.0 22.5 47.5 10.0 42 27.500000000000004 20.0 20.0 32.5 43 25.0 20.0 40.0 15.0 44 15.0 35.0 27.500000000000004 22.5 45 22.5 35.0 22.5 20.0 46 20.0 22.5 32.5 25.0 47 7.5 32.5 35.0 25.0 48 25.0 32.5 15.0 27.500000000000004 49 27.500000000000004 22.5 25.0 25.0 50 2.5 27.500000000000004 32.5 37.5 51 17.5 27.500000000000004 32.5 22.5 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.5 27 1.0 28 1.5 29 2.0 30 3.5 31 5.0 32 3.5 33 2.0 34 2.5 35 3.0 36 1.5 37 0.0 38 3.0 39 6.0 40 4.0 41 2.0 42 2.0 43 2.0 44 3.0 45 4.0 46 3.0 47 2.0 48 2.0 49 2.0 50 2.5 51 3.0 52 3.5 53 4.0 54 2.5 55 1.0 56 1.0 57 1.0 58 0.5 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 40.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGACTCGATGAGTTAAAGATAGCTTCTCGTCAATAAGATCCTGGTATCTCT 1 2.5 No Hit GAGGAAGGATATCTTGCAATTTTTTGGTCTGAGGAGAACCGCTAACAACCC 1 2.5 No Hit ATTGTAGCATCTGTATTTCTGTCACCATTGAAAATCAGTTAGAAAACATCA 1 2.5 No Hit ATCTCTCTTTTTGCGTTCTGCTTCAATATCTGGTTGAACGGCGTCGCGTCG 1 2.5 No Hit GTTCTTCCGTCTTTCAATTTCACCTCAGTGACCTCTCCATTTGAGTTGGTA 1 2.5 No Hit GTGTAAAGAGTGTAATCCAAATTATCATCACCGACATCAATGGAATCAAAG 1 2.5 No Hit CTTAGTGACATTCCAGATGCGACCAGTACGACCTGTCTCTTATACACATCT 1 2.5 No Hit ACCACCGGCGACTCTGATCTCATCTCCTCAACCTCCGCATTAACCGGAGAC 1 2.5 No Hit CAAACATATGATGATGTTGATGATGATGATCAAGAACCGATCTAAATGAAA 1 2.5 No Hit TAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACA 1 2.5 No Hit ATCAAAGACATGCAACCTACGGAAACTACTCCAATGCTGCATTTCAATTTC 1 2.5 No Hit TCTTTTTTCTTATCTCTTGTTGTTTGATTCTTCAGGAACTTGTTAGAATCT 1 2.5 No Hit GTATCAACGCAGAGTCGGGGACCGAATCCAACCTAATACATGGAGTCTGTC 1 2.5 No Hit CCTTAAGCTTGCTCGGTAGGTCTAAGTCGCCGGCTCATGCTTCAATAGGCA 1 2.5 No Hit TTGAAGGAGTTCCTACTCCATATGACAAGATCAAGAGGATGGTCGTTCCTG 1 2.5 No Hit TTCCAATCCATTCCATTCCTTTCCATTCCACTCGAGTTGATTTCATTCCAT 1 2.5 No Hit GTGCTGGTGTGCTTTCCGGTTACGACAAATTGCAGCTGATTGTCTTCGGTA 1 2.5 No Hit GTACACGTATGTCTCCAGGGCATTCCTGGCGTCGATCTTCTCCTTCACCTT 1 2.5 No Hit TTAATAGACAAAGGAAGATCAAACTGAAAATCCATTAATCCAACAACAGCA 1 2.5 No Hit ACTATAGACAGTATAAAATCAACTGAAAAACAGTTTGTATTAATCCTGTCT 1 2.5 No Hit GCCCATATAAAGTCTTAACGGGCCAGACTAATTTAGTAAAACCATCTCCTA 1 2.5 No Hit TCATGAAACAAATACAATTGTAACACAAGAATAAAAGTGAGGCAGGTTTAT 1 2.5 No Hit ACATAACTGGATGACTGATGACCATAAAAGCATGTTGATCTCCACCTCTTC 1 2.5 No Hit ATCATCGTCGTCTTCATCATCAATATCAGCCCAAGACTTAACTTTCAAAGG 1 2.5 No Hit CTCTTCGTTCACGATGTGATCATTTCTGAATTTTTGGTTTTAGTACAAACT 1 2.5 No Hit GACTTTATGATAAAAGTAACCACATGCCATTTGTGTGATAAAAGCTTTGTA 1 2.5 No Hit CTGCTTCTCCCGTGTTTGTCCAAACCCCAGGAGAGAAGGGGTTCACTAACT 1 2.5 No Hit ATGGTGTACTCTCTGGTAATCACTTCCTCTTTCCTTCCCTTTTTCTCAGAC 1 2.5 No Hit CTGGTCTAGCTCCATGGCACCGAAAATGTATCAGGGCTCAAGTGATTCACC 1 2.5 No Hit CAACGCGTCAGTTTTTGACAGAATCGTTAGTTGATGGCGAAAGGTCGCAAA 1 2.5 No Hit CTTGACAAAGAAGAAACACAATGTCTATTTGCTCATAAAATTTTGAGAGAC 1 2.5 No Hit CATCGCAATCATGCCTTGATGAACGATATCACCCGCACCCGCTGACACCTG 1 2.5 No Hit GCTCTAGCTGTCTATGTTTTATCCAAGGGCCATCATCACGGCGAAGACCCT 1 2.5 No Hit GATCTATTCCTGAAGGAGCTGTTGTCTGCAACGTCGAGCATCACGTCGGTG 1 2.5 No Hit GGTTAAAACTTATTGTTGATTCTTCTCTATTCGTCGTCGACCTATTTTCCT 1 2.5 No Hit CAGTATACGTGGAAAGGGTTTTCCGCAATTCCAGGAGCATTGAAGGAGCTA 1 2.5 No Hit GAGAACTACTTAATGCTGTTCACATTAAAAACAATCAGACAGGTGAAGAAC 1 2.5 No Hit CTTATAATCCACGACAAAAAGGTAAGATCTTAACCGACAAAATCTACTCTT 1 2.5 No Hit ACATAATTCCCTTCATATTCCAGATTGCTGAAAAACTTAAGCGGGGAAGAG 1 2.5 No Hit GTCCAAGTGGAAGGATGTAGTTAGTTTTAGTAGGCAAATCCGCTAGACTAT 1 2.5 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 2.5 34 0.0 0.0 2.5 35 0.0 0.0 2.5 36 0.0 0.0 2.5 37 0.0 0.0 2.5 38 0.0 0.0 2.5 39 0.0 0.0 2.5 >>END_MODULE >>Kmer Content pass >>END_MODULE