FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_62.3410000000a9c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_62.3410000000a9c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTCGATGAGTTAAAGATAGCTTCTCGTCAATAAGATCCTGGTATCTCT12.5No Hit
GAGGAAGGATATCTTGCAATTTTTTGGTCTGAGGAGAACCGCTAACAACCC12.5No Hit
ATTGTAGCATCTGTATTTCTGTCACCATTGAAAATCAGTTAGAAAACATCA12.5No Hit
ATCTCTCTTTTTGCGTTCTGCTTCAATATCTGGTTGAACGGCGTCGCGTCG12.5No Hit
GTTCTTCCGTCTTTCAATTTCACCTCAGTGACCTCTCCATTTGAGTTGGTA12.5No Hit
GTGTAAAGAGTGTAATCCAAATTATCATCACCGACATCAATGGAATCAAAG12.5No Hit
CTTAGTGACATTCCAGATGCGACCAGTACGACCTGTCTCTTATACACATCT12.5No Hit
ACCACCGGCGACTCTGATCTCATCTCCTCAACCTCCGCATTAACCGGAGAC12.5No Hit
CAAACATATGATGATGTTGATGATGATGATCAAGAACCGATCTAAATGAAA12.5No Hit
TAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACA12.5No Hit
ATCAAAGACATGCAACCTACGGAAACTACTCCAATGCTGCATTTCAATTTC12.5No Hit
TCTTTTTTCTTATCTCTTGTTGTTTGATTCTTCAGGAACTTGTTAGAATCT12.5No Hit
GTATCAACGCAGAGTCGGGGACCGAATCCAACCTAATACATGGAGTCTGTC12.5No Hit
CCTTAAGCTTGCTCGGTAGGTCTAAGTCGCCGGCTCATGCTTCAATAGGCA12.5No Hit
TTGAAGGAGTTCCTACTCCATATGACAAGATCAAGAGGATGGTCGTTCCTG12.5No Hit
TTCCAATCCATTCCATTCCTTTCCATTCCACTCGAGTTGATTTCATTCCAT12.5No Hit
GTGCTGGTGTGCTTTCCGGTTACGACAAATTGCAGCTGATTGTCTTCGGTA12.5No Hit
GTACACGTATGTCTCCAGGGCATTCCTGGCGTCGATCTTCTCCTTCACCTT12.5No Hit
TTAATAGACAAAGGAAGATCAAACTGAAAATCCATTAATCCAACAACAGCA12.5No Hit
ACTATAGACAGTATAAAATCAACTGAAAAACAGTTTGTATTAATCCTGTCT12.5No Hit
GCCCATATAAAGTCTTAACGGGCCAGACTAATTTAGTAAAACCATCTCCTA12.5No Hit
TCATGAAACAAATACAATTGTAACACAAGAATAAAAGTGAGGCAGGTTTAT12.5No Hit
ACATAACTGGATGACTGATGACCATAAAAGCATGTTGATCTCCACCTCTTC12.5No Hit
ATCATCGTCGTCTTCATCATCAATATCAGCCCAAGACTTAACTTTCAAAGG12.5No Hit
CTCTTCGTTCACGATGTGATCATTTCTGAATTTTTGGTTTTAGTACAAACT12.5No Hit
GACTTTATGATAAAAGTAACCACATGCCATTTGTGTGATAAAAGCTTTGTA12.5No Hit
CTGCTTCTCCCGTGTTTGTCCAAACCCCAGGAGAGAAGGGGTTCACTAACT12.5No Hit
ATGGTGTACTCTCTGGTAATCACTTCCTCTTTCCTTCCCTTTTTCTCAGAC12.5No Hit
CTGGTCTAGCTCCATGGCACCGAAAATGTATCAGGGCTCAAGTGATTCACC12.5No Hit
CAACGCGTCAGTTTTTGACAGAATCGTTAGTTGATGGCGAAAGGTCGCAAA12.5No Hit
CTTGACAAAGAAGAAACACAATGTCTATTTGCTCATAAAATTTTGAGAGAC12.5No Hit
CATCGCAATCATGCCTTGATGAACGATATCACCCGCACCCGCTGACACCTG12.5No Hit
GCTCTAGCTGTCTATGTTTTATCCAAGGGCCATCATCACGGCGAAGACCCT12.5No Hit
GATCTATTCCTGAAGGAGCTGTTGTCTGCAACGTCGAGCATCACGTCGGTG12.5No Hit
GGTTAAAACTTATTGTTGATTCTTCTCTATTCGTCGTCGACCTATTTTCCT12.5No Hit
CAGTATACGTGGAAAGGGTTTTCCGCAATTCCAGGAGCATTGAAGGAGCTA12.5No Hit
GAGAACTACTTAATGCTGTTCACATTAAAAACAATCAGACAGGTGAAGAAC12.5No Hit
CTTATAATCCACGACAAAAAGGTAAGATCTTAACCGACAAAATCTACTCTT12.5No Hit
ACATAATTCCCTTCATATTCCAGATTGCTGAAAAACTTAAGCGGGGAAGAG12.5No Hit
GTCCAAGTGGAAGGATGTAGTTAGTTTTAGTAGGCAAATCCGCTAGACTAT12.5No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers