##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_3_61.3410000000a9a9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.0 0.0 0.0 0.0 0.0 0.0 2 38.0 0.0 0.0 0.0 0.0 0.0 3 37.53846153846154 0.0 0.0 0.0 0.0 0.0 4 38.0 0.0 0.0 0.0 0.0 0.0 5 37.53846153846154 0.0 0.0 0.0 0.0 0.0 6 39.84615384615385 0.0 0.0 0.0 0.0 0.0 7 39.84615384615385 0.0 0.0 0.0 0.0 0.0 8 39.84615384615385 0.0 0.0 0.0 0.0 0.0 9 39.84615384615385 0.0 0.0 0.0 0.0 0.0 10 40.0 0.0 0.0 0.0 0.0 0.0 11 40.0 0.0 0.0 0.0 0.0 0.0 12 39.53846153846154 0.0 0.0 0.0 0.0 0.0 13 39.53846153846154 0.0 0.0 0.0 0.0 0.0 14 39.69230769230769 0.0 0.0 0.0 0.0 0.0 15 39.69230769230769 0.0 0.0 0.0 0.0 0.0 16 40.0 0.0 0.0 0.0 0.0 0.0 17 39.84615384615385 0.0 0.0 0.0 0.0 0.0 18 39.84615384615385 0.0 0.0 0.0 0.0 0.0 19 40.0 0.0 0.0 0.0 0.0 0.0 20 40.0 0.0 0.0 0.0 0.0 0.0 21 39.69230769230769 0.0 0.0 0.0 0.0 0.0 22 39.69230769230769 0.0 0.0 0.0 0.0 0.0 23 40.0 0.0 0.0 0.0 0.0 0.0 24 39.53846153846154 0.0 0.0 0.0 0.0 0.0 25 40.0 0.0 0.0 0.0 0.0 0.0 26 39.69230769230769 0.0 0.0 0.0 0.0 0.0 27 39.84615384615385 0.0 0.0 0.0 0.0 0.0 28 39.84615384615385 0.0 0.0 0.0 0.0 0.0 29 37.76923076923077 0.0 0.0 0.0 0.0 0.0 30 38.69230769230769 0.0 0.0 0.0 0.0 0.0 31 37.61538461538461 0.0 0.0 0.0 0.0 0.0 32 38.84615384615385 0.0 0.0 0.0 0.0 0.0 33 39.69230769230769 0.0 0.0 0.0 0.0 0.0 34 39.84615384615385 0.0 0.0 0.0 0.0 0.0 35 38.84615384615385 0.0 0.0 0.0 0.0 0.0 36 39.69230769230769 0.0 0.0 0.0 0.0 0.0 37 39.53846153846154 0.0 0.0 0.0 0.0 0.0 38 39.38461538461539 0.0 0.0 0.0 0.0 0.0 39 39.53846153846154 0.0 0.0 0.0 0.0 0.0 40 39.53846153846154 0.0 0.0 0.0 0.0 0.0 41 39.69230769230769 0.0 0.0 0.0 0.0 0.0 42 39.84615384615385 0.0 0.0 0.0 0.0 0.0 43 39.69230769230769 0.0 0.0 0.0 0.0 0.0 44 37.92307692307692 0.0 0.0 0.0 0.0 0.0 45 37.30769230769231 0.0 0.0 0.0 0.0 0.0 46 38.69230769230769 0.0 0.0 0.0 0.0 0.0 47 37.76923076923077 0.0 0.0 0.0 0.0 0.0 48 37.76923076923077 0.0 0.0 0.0 0.0 0.0 49 38.84615384615385 0.0 0.0 0.0 0.0 0.0 50 38.69230769230769 0.0 0.0 0.0 0.0 0.0 51 37.61538461538461 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2204 1 0.0 2204 2 0.0 2204 3 0.0 2204 4 0.0 2204 5 0.0 2204 6 0.0 2204 7 0.0 2204 8 0.0 2204 9 0.0 2204 10 0.0 2204 11 0.0 2204 12 0.0 2204 13 0.0 2204 14 0.0 2204 15 0.0 2204 16 0.0 2204 17 0.0 2204 18 0.0 2204 19 0.0 2204 20 0.0 2204 21 0.0 2204 22 0.0 2204 23 0.0 2204 24 0.0 2204 25 0.0 2204 26 0.0 2204 27 0.0 2204 28 0.0 2204 29 0.0 2204 30 0.0 2204 31 0.0 2204 32 0.0 2204 33 0.0 2204 34 0.0 2204 35 0.0 2204 36 0.0 2204 37 0.0 2204 38 0.0 2204 39 0.0 2204 40 0.0 2204 41 0.0 2204 42 0.0 2204 43 0.0 2204 44 0.0 2204 45 0.0 2204 46 0.0 2204 47 0.0 2204 48 0.0 2204 49 0.0 2204 50 0.0 2204 51 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 36 1.0 37 1.0 38 1.0 39 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.76923076923077 23.076923076923077 7.6923076923076925 38.46153846153847 2 23.076923076923077 15.384615384615385 30.76923076923077 30.76923076923077 3 23.076923076923077 15.384615384615385 46.15384615384615 15.384615384615385 4 0.0 23.076923076923077 38.46153846153847 38.46153846153847 5 7.6923076923076925 46.15384615384615 23.076923076923077 23.076923076923077 6 23.076923076923077 46.15384615384615 23.076923076923077 7.6923076923076925 7 23.076923076923077 30.76923076923077 15.384615384615385 30.76923076923077 8 23.076923076923077 30.76923076923077 38.46153846153847 7.6923076923076925 9 38.46153846153847 15.384615384615385 7.6923076923076925 38.46153846153847 10 0.0 46.15384615384615 38.46153846153847 15.384615384615385 11 23.076923076923077 46.15384615384615 15.384615384615385 15.384615384615385 12 23.076923076923077 15.384615384615385 7.6923076923076925 53.84615384615385 13 15.384615384615385 38.46153846153847 15.384615384615385 30.76923076923077 14 7.6923076923076925 38.46153846153847 23.076923076923077 30.76923076923077 15 23.076923076923077 30.76923076923077 15.384615384615385 30.76923076923077 16 0.0 46.15384615384615 38.46153846153847 15.384615384615385 17 7.6923076923076925 23.076923076923077 53.84615384615385 15.384615384615385 18 38.46153846153847 23.076923076923077 23.076923076923077 15.384615384615385 19 15.384615384615385 30.76923076923077 46.15384615384615 7.6923076923076925 20 23.076923076923077 15.384615384615385 38.46153846153847 23.076923076923077 21 30.76923076923077 30.76923076923077 7.6923076923076925 30.76923076923077 22 7.6923076923076925 38.46153846153847 30.76923076923077 23.076923076923077 23 23.076923076923077 15.384615384615385 46.15384615384615 15.384615384615385 24 15.384615384615385 7.6923076923076925 46.15384615384615 30.76923076923077 25 7.6923076923076925 38.46153846153847 38.46153846153847 15.384615384615385 26 23.076923076923077 23.076923076923077 46.15384615384615 7.6923076923076925 27 7.6923076923076925 46.15384615384615 23.076923076923077 23.076923076923077 28 7.6923076923076925 38.46153846153847 30.76923076923077 23.076923076923077 29 15.384615384615385 30.76923076923077 38.46153846153847 15.384615384615385 30 46.15384615384615 7.6923076923076925 30.76923076923077 15.384615384615385 31 23.076923076923077 15.384615384615385 23.076923076923077 38.46153846153847 32 30.76923076923077 15.384615384615385 23.076923076923077 30.76923076923077 33 15.384615384615385 30.76923076923077 23.076923076923077 30.76923076923077 34 7.6923076923076925 38.46153846153847 38.46153846153847 15.384615384615385 35 30.76923076923077 15.384615384615385 30.76923076923077 23.076923076923077 36 15.384615384615385 30.76923076923077 38.46153846153847 15.384615384615385 37 23.076923076923077 30.76923076923077 38.46153846153847 7.6923076923076925 38 0.0 38.46153846153847 30.76923076923077 30.76923076923077 39 30.76923076923077 23.076923076923077 23.076923076923077 23.076923076923077 40 7.6923076923076925 23.076923076923077 23.076923076923077 46.15384615384615 41 30.76923076923077 30.76923076923077 7.6923076923076925 30.76923076923077 42 38.46153846153847 15.384615384615385 38.46153846153847 7.6923076923076925 43 30.76923076923077 7.6923076923076925 38.46153846153847 23.076923076923077 44 15.384615384615385 23.076923076923077 30.76923076923077 30.76923076923077 45 0.0 23.076923076923077 53.84615384615385 23.076923076923077 46 15.384615384615385 30.76923076923077 38.46153846153847 15.384615384615385 47 7.6923076923076925 15.384615384615385 61.53846153846154 15.384615384615385 48 23.076923076923077 38.46153846153847 15.384615384615385 23.076923076923077 49 23.076923076923077 23.076923076923077 30.76923076923077 23.076923076923077 50 15.384615384615385 15.384615384615385 30.76923076923077 38.46153846153847 51 0.0 38.46153846153847 30.76923076923077 30.76923076923077 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 0.5 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 1.0 31 2.0 32 1.5 33 1.0 34 1.0 35 1.0 36 1.0 37 1.0 38 1.0 39 1.0 40 0.5 41 0.0 42 0.5 43 1.0 44 0.5 45 0.0 46 0.5 47 1.0 48 0.5 49 0.0 50 0.0 51 0.0 52 0.5 53 1.0 54 1.0 55 1.0 56 1.0 57 1.0 58 0.5 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.5 71 1.0 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 13.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTAGCCAAGAAACAGCGACAGAGAAAGTGAAAATTAAGATGTAAAATT 1 7.6923076923076925 No Hit GTTCTACTTATGACATCAGGATTGTGAATGTGTTGATTGGAGCTTATGCTC 1 7.6923076923076925 No Hit GAGTACGGGCAACATTAGTGGCTCAATCGTCGAGGTGAGCCAGCCAAACAA 1 7.6923076923076925 No Hit GGCTAGCAATCCAGGACCTCCTTTCTCCATGGCTGAGCACAGGCATTCTGA 1 7.6923076923076925 No Hit CCTCCTTAGCACCCAATTTTGCCAATTATCGCGCTTCCTTATGAAGTCACC 1 7.6923076923076925 No Hit AGTTAAATCTACCATTTGACCATTAAATTTACCTGCTACAGCTTTCTTTCT 1 7.6923076923076925 No Hit CCAAAGAAATCTAACATTAAGTCTTTGAAGAACACAAAGCATAATCTAGCA 1 7.6923076923076925 No Hit CTGTAACACAACTCCTAAATAAGTTTCAAGCAAAATTATCCGGAAACTGCA 1 7.6923076923076925 No Hit CGACCTATGAACCACATCTTAAACGTAGCGCTTTCTACCTCTTCTTCACTT 1 7.6923076923076925 No Hit ATGATGATGAGGATGATGAAGAGTAAATCACCAATCAAGCCTTCTTTGTCT 1 7.6923076923076925 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA 1 7.6923076923076925 No Hit ACCAGTGGCTGCTCGAGAGCCAGCTGCTGGCCGATGGTCCGGCGCTGGTGC 1 7.6923076923076925 No Hit CTTCCATTGTTCGTCTTGTTCGTCCCACTCGTCCCGACAAGGCTCGTCGTC 1 7.6923076923076925 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 0.0 36 0.0 0.0 0.0 37 0.0 0.0 0.0 38 0.0 0.0 0.0 39 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE