FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_6.3410000000a5fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_6.3410000000a5fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC32.1739130434782608No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC21.4492753623188406TruSeq Adapter, Index 12 (95% over 21bp)
CTCTTGTAGTGTTGGAGAATAGGACAAATGAAGAAGTTCTACATGCGACTA10.7246376811594203No Hit
GTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTTACGCAATG10.7246376811594203No Hit
AGGTAGGAGTGAGGTTCTTCCTCCTGCAGCCACCAGGCAGCTGATCACCAT10.7246376811594203No Hit
ACTGAAGAACAATGGTTTTGCTGTTGAGAAGAAGATCATGACTTCAACTGT10.7246376811594203No Hit
GTCCTCTGCACAAACCTAGGAGTCGCACAACCACCTCCTCTCAGAGGAATC10.7246376811594203No Hit
CTAGAGAACAGTCTGATTTCCACCTTGGTTCAAAGACTGTCTCTTATACAC10.7246376811594203No Hit
GAAGCAAGCTGTGAAGAAGGATAGTTTTCAGTTGTTTGCTGAGAAAGTTAG10.7246376811594203No Hit
ACATTACACATTCGATGCTGCCAATGACACAAAGGATACTGGCTGTCTCTT10.7246376811594203No Hit
GTTCTATGTGATCATGTTTCATATGTTTAGGCCTGAGGCTGTCTCTTATAC10.7246376811594203No Hit
CTTTTACACTCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT10.7246376811594203Illumina PCR Primer Index 8 (95% over 21bp)
GTCATGACCTGCTAAGTTCCAGTATGTATGTTGCTGTCTCTTATACACATC10.7246376811594203No Hit
AAGAAGCAGGAGAATAATGGAGTCTTGTGTGTTTTCTTCGGTGTGCGTCTT10.7246376811594203No Hit
GCGTGCAGCAGCAGGACGAGGGCGAGGGGCCTGTCTCTTATAATCTCCGAG10.7246376811594203No Hit
GGATCGTCAAAACTCCAGGAGGTAAATTGACGTACCAGACCACTAAGAAGA10.7246376811594203No Hit
ATCCTGATGCATACAGAGCACCGCTTCTTTATTTGAGGTTGGTTTCATGGC10.7246376811594203No Hit
GGTATCAACGCAGAGTACGGGGGTAACGGAGGAAGAAGAAGATCTGCCTGT10.7246376811594203No Hit
GTCCAGAATAATAAGCCGTATCAATTCCATTATCAGGTTCAGTGTCTCTTA10.7246376811594203No Hit
TAGCAACAACAGGGCCGGATACGATGTAGTCAACGAGTCCACTGAAGAAAG10.7246376811594203No Hit
GGGATGCAAGGATTGATCCTCCGATCCAGACACTGTATTTTCTCTCTGGCG10.7246376811594203No Hit
CCATTATAGTATACACCAACTTGGGTTTATTAGAGTTTGATGTTGCTGTTG10.7246376811594203No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC10.7246376811594203TruSeq Adapter, Index 12 (95% over 24bp)
GATCAATAAGAACGACGAAGAACCTTAGAAGGTGAAAATAGGTTAATCCCT10.7246376811594203No Hit
CTTTTATACATCCTCAAATCGATTTTCCTCAAGTACGGTGCTCCGTCCATT10.7246376811594203No Hit
GATATTCGAATGAGTCAAATTCAACAGCGATACATAATAGAGAGATAAAAG10.7246376811594203No Hit
CAAGAAGCCTTACGGATTCAAAATCAACGCTTCATACAAAGTAGCTGTTCT10.7246376811594203No Hit
GCCTTGTGATGGGTAACAGAGGCCTTGGTGGTCTTAAGCTGTCTCTTATAC10.7246376811594203No Hit
GATCTGTATCTAAGATCATTGCTTCTTCCGAAGCATCAGTCTCCAGATCTG10.7246376811594203No Hit
CATTTTAAGAAAAACAGACCAACTAAAGAGGTCTCTCTACCTGTCTCTTAT10.7246376811594203No Hit
ATCTCATTTCGGGATGCTTCTAGAGAACGAGGACGGTTCAGATATAACCTG10.7246376811594203No Hit
GTGATGCCCTTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACCTTGC10.7246376811594203No Hit
GAGTACGGGGACGTCAAACTCTCTCTATACACAGAGTTAAATTGAGTTTGT10.7246376811594203No Hit
CCTTGTAGTAAGCAAAGACCAAAGTGCTGTCATCATGCATACCCTCCCCAA10.7246376811594203No Hit
GTACACATTCTCGCCAACTTTCCATCCCGGAACATCTTTCATTACATCAGC10.7246376811594203No Hit
CGCCAAGCATAAGTACCGTATATTGCATCTTGTGCGGGCTCTAAAGGATGC10.7246376811594203No Hit
CTTCTGGTTTCTGTAAGTGTTGCTTGAACCTTGCCACGATGCCGAGCACTA10.7246376811594203No Hit
ATGTAGGAATTGGGAGTTTTTTTCAAGATTTCTTCAGCCTTCTGAATTGCT10.7246376811594203No Hit
CCCTTGAGGAAAGGGTACAAGTGAACAATCACCCAGAAGGCAAAGAACAAC10.7246376811594203No Hit
ACTTCGTCTCAAGATAAAATCAAAACCAACAGAGAGTCTAGAATAAAAGTG10.7246376811594203No Hit
GTGTAGGTAGGTCTCTCAATGTTAAGGGAGCGTCTACAGATGTCATAGATC10.7246376811594203No Hit
CCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGATCCGCACTCCTTGCGC10.7246376811594203No Hit
CCACCACAACCGTAGAAACTTCAGCCGGTTTTAAGCGGCTTTCCAAGCAGA10.7246376811594203No Hit
CACAAACTGAGACAAGCAGCGAGTCTGTAAAAGAAACGGGACTTGATGACT10.7246376811594203No Hit
GTGTGAAGCTATATCGATCATCAGTTCACGGATTAGACTCTGAAGAAGGTT10.7246376811594203No Hit
CCATCTCAAAGATGGGTTTCTCTCCGACAGATGTGTCAGGGTCCGACCCCA10.7246376811594203No Hit
GTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCCTGTCTCTTATACAC10.7246376811594203No Hit
CCCCTTATGTCCAGGGCTTCACACATGCTACAATGGCCAGTACAGAGGGCT10.7246376811594203No Hit
GATAAAAGCAATAAAATGCTAAGAAAGAAGAACCAAACCCAAAATATCAAT10.7246376811594203No Hit
CAACGAGGAAGCTTGGAGGTAAAAAACCAAACATTGATAGAGTTACATTAC10.7246376811594203No Hit
GTTCCTTGGCTCGTGCCGGTAAGGTGAGAGGTCAGACACCGAAAGTGGCAA10.7246376811594203No Hit
GAAAAACATGAATGGTTAAAAGTTGAGCCTGCAAAGAGAAAACAAGAACTA10.7246376811594203No Hit
GCATATACAGATCGTGTGGTGGAGGCAGTTGAAAATTCTTATCTCTGTCTC10.7246376811594203No Hit
GGTTAATCCCGGTACTGATGATAACAGCAATAATCAAACCATAGATACCCA10.7246376811594203No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCTGAATCTCGTATGCCGTC10.7246376811594203No Hit
AGGCATTACAGAGGAGTGAGGATGAGGCCGTGGGGGAAATTCGCGGCGGAG10.7246376811594203No Hit
GACGAAGAAACTGGAAAGATACTTAATTTAGTATTCGTATGGGCTGATGAT10.7246376811594203No Hit
TCCATGTACCTCTGGCATCAAATTCCTCAGGCATAGTTCAACCTGAATATG10.7246376811594203No Hit
ATACAGATCCTCAGGGATCTCAGGTGCAAGACCATGAGCTTTCAAAATTCT10.7246376811594203No Hit
GCACATGAGCTTCCTCAGTATGGACTCACTGTTGGTCTGTATCTTATACAC10.7246376811594203No Hit
GCTTGACACAGTGAGTTCTTCCCACGCTGTGTGACCCTAGGAGGGCGACGA10.7246376811594203No Hit
GCTCGTTACTTTGGATATCGATGCTTTCAACTTCACCAGAGAAAGAGAAAA10.7246376811594203No Hit
AATAGTGACAATGCTGTGTTGAGCCATGAGCTACGGTGTGATCTCTGTCTC10.7246376811594203No Hit
CCCCATTCGACCCGTCTTGAAACACGGACCAAGGAGTCTGACATGTGTGCG10.7246376811594203No Hit
CAATGAGTCATCTTTAGAGTTTCAGAGGAAGATTCTTGAACGTTCTGGTTT10.7246376811594203No Hit
ACTCCACATAGACCACATCTCCTAAATGGTCCTGCGCGTGATCCGTTATAC10.7246376811594203No Hit
ACAGAGGAGAAATTTCCAGAGTTCAAGAGATGGGTTAGGAATTTGGAGAAG10.7246376811594203No Hit
GCATATAAGGGACCTTATCAAACATTTCGTAGCTGTCTCTTATACACATCT10.7246376811594203No Hit
CTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTAAA10.7246376811594203No Hit
TCATTGGCCAATGCAGGCCGTTGTCGAAGACGGTTAGGTTCAATGTGCTGA10.7246376811594203No Hit
CTCCGAGCAAACCGAAAGATACGATGAAATGGTGGAGGCGATGACTGTCTC10.7246376811594203No Hit
CTCCCGGAGAATAAGGACATACCAAATCAAGCAACCGGAGATCCAACGGTG10.7246376811594203No Hit
CAAAGAGCGTCTGAGCAAAGGAGACAGTGTGAAAGTGGAGAAGAAGAAGAG10.7246376811594203No Hit
GTCTCAGCAATTCCAATTACCTCAAGGTAAGTAGTTAAAGGCGGTTGAGGA10.7246376811594203No Hit
GCCAAGCGCGACGAGAAAACTGTCAACGCCACTCGTCGATCCGGCGCCGAT10.7246376811594203No Hit
AAGGAGTACCAAATCATAGATCATCTTGTTGGTCCTACGTTGAAAGACGCG10.7246376811594203No Hit
GTCGTGGAGGGCGTGCCAGTGAAGAAGAGAAATGGGTTCCAGTGACCAAGC10.7246376811594203No Hit
ATCTAAGGCTATCACGGACCAGTATTCATCATCGAGTAAACTCAGGTAAAC10.7246376811594203No Hit
GGTATCAACGCAGAGTACGGGGCCTGCTGCTCGATACATTGTCTGTCTCTT10.7246376811594203No Hit
TCGTAGTACAACTAAAAATCTTACACAACAAGCTCCAACTGTCAAAAACCT10.7246376811594203No Hit
CCTCGTTGGATTGTTTGAAGACACCAATCTTTGCGCGATTCATGCTAAGAG10.7246376811594203No Hit
TCAACGACGCTTCGGCGAGATGCTACAAAGCCGAAGACGACGTCGAGGAGG10.7246376811594203No Hit
CAAGAAAGCTGGAACCTTTGATCCAAAGAAACTCATGGGTGTCACTATGCT10.7246376811594203No Hit
CCTCATGAGTCTCACAAGCCTCAGACCAAGAAGACTCATCACAAGTTGATG10.7246376811594203No Hit
TAGCAGCCATTTGTTCAAGTCGCAACTTTTCAGCAGCTTTTTGTTCACGCT10.7246376811594203No Hit
CTATGGTGTTGAGCTTGGGCGCAAGAAGAAGAGTGCTTCTTTCACCAAGAA10.7246376811594203No Hit
GTGTACAGGTTGAGGAGCTGGGTTGCGTGCAGCAGCAGGACTGTCTCTTAT10.7246376811594203No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT10.7246376811594203No Hit
GTGGTGGATTCTCTACTATTGCTGCCAAGAGTTCATAAAAGCTTGGCGGGT10.7246376811594203No Hit
TTGATAGACCTTATGGAAGATTTCAAAGGAGAAGTGAGATCAGAAGGAAGA10.7246376811594203No Hit
GTGCTATGTTGAGGAGCTCCAGTACCGTAATTGGCGTTTGCGTTTGTGCTA10.7246376811594203No Hit
AGCTATAGTAGGAGGATTCTCTCTTTTCATGGCTGCTCCTCTCATCAGTGG10.7246376811594203No Hit
AACATTCCCTGTTATGCGAAGATATGACAGACATACAATATCTCAAGCAGA10.7246376811594203No Hit
GGCTGATCATTTGGGTAACAACAATGGTGGTTCTTCTTCATCTCCAGTTAA10.7246376811594203No Hit
TGTGAGTAGAGACATTGCTTCCTCCTCCAAGTTTCCGGTAACGCTTCGTTC10.7246376811594203No Hit
GAGTGAAGGCACTCCTTGGCTTCTCATCTGCTCTTGTGCGCTCTTCATTTC10.7246376811594203No Hit
CTCTTTGTTCAAGGGAGTACTTGATTACACAGAATTCAATGATGTGGACAT10.7246376811594203No Hit
GACAAAAGAAGAAGAAAATGTTAGCAAGTAATTTATTTGCTACTTTAAGTA10.7246376811594203No Hit
GTCTCAATGATATTGATGAGGACTCTCAGAAATGATTATGCGAAGTATGCT10.7246376811594203No Hit
AATTATTAGAGCGAAGAACAAAGAGTATGAAATGATAAGAGCTGTATCTTA10.7246376811594203No Hit
GATTAATGAAGTTTTGAAACATTACCCACAATGTTATCACGGTGTTGACAA10.7246376811594203No Hit
GATTTACTTGTTTACTCTGATAATCTGTGTTCGTGGTGTCCTAAGTTAAAA10.7246376811594203No Hit
TCCCAAGGCCCCCGCCGACGTCTCCGGACTCCCTAACGTCTGTCTCTTATA10.7246376811594203No Hit
GGAATCCATTGTTGCTTCTGAGGTTACAGAGAAGCTAGATGCTGTCTCTTA10.7246376811594203No Hit
GATTAAAAAACCAAAAGCAAGAAGAGTCATAGACTCAAAAACATAACAAGA10.7246376811594203No Hit
AGTAATTTATTTGCTACTTTAAGTACTTTGTGTCTTGTTATGTCTGTCTCT10.7246376811594203No Hit
TCGGACACCTTCACACTTAGGATGCTGGCGTAATGGCTTTAAGTGGCCCGT10.7246376811594203No Hit
ATAATGGAGAAGTTAGAAAAAAGCAAGATGGATATTCAGCTCAAGAGATGA10.7246376811594203No Hit
CAACAAGATGATGCACCTTCGTATATTCAAGAAAATACCAAGGAAGGAGAC10.7246376811594203No Hit
GAATAGAATGGGAAGGAATGGAATGGAATGGAATTAACCCGAACAGAATGG10.7246376811594203No Hit
GAAGAAGCACTCTTCTTCTTGCGCCCAAGCTCAACACCATAGTGCGAGAGG10.7246376811594203No Hit
GTACTTTGTGTCTTGTTATGTTTTTGAGTCTATGACTCTTCTTGCTTTTGG10.7246376811594203No Hit
CTCCTACTCCTCTTGGAGAAGGCAAGAGTACTACCACTGTTGGACTTTGCC10.7246376811594203No Hit
GCCATCTTTAGACAAGTGCTTCAAGGATTAATCTATCTTCATCCTGTCTCT10.7246376811594203No Hit
GTACCTCACTCCGCAAGCGTTACAAAGCGTCTTCGGACCCATAGGTCCCGT10.7246376811594203No Hit
CTCACAAGTCCATACTCCCAAAAAAACCTTACGATGATTCCAAATTGAAAA10.7246376811594203No Hit
CACGAAGCGCCACCAGATTTTCGGGTACGGGACTCTCACCCCCTACGGCCT10.7246376811594203No Hit
GTCAATACGCGATTCAGAAATTCATAGAGGAGAAACATAAATCACTGCCTC10.7246376811594203No Hit
CCTTGGAACTTACCGTGGTCACAACGGTGCTGTTTGGTGCTGTGATGTCTC10.7246376811594203No Hit
ACCTGATATGCCTCTTAACTGGATATGACACTTTTTATGAAGTATGCTTCT10.7246376811594203No Hit
GATCGATTGTGGGAGTGCGTGTGTAGCACGGTGCAGGCTTTCGAGGAGGCC10.7246376811594203No Hit
CTCGAGGGTAACCAGTTGGCTGAGGCTGATGAGTTCGAAGACAAGATGAAG10.7246376811594203No Hit
ACCGAAAGAACTTATGGTTTGTCTTATTTTTCAAAAGAAACAGATAATGTA10.7246376811594203No Hit
CAGTTGTAGCTATTCCTGTATCACCAACACATTCAAATGAGAAATCTGCAC10.7246376811594203No Hit
CTTCAAGTCATTGAGCTTTGTTCTTCTGCATCTCTTTGGCTTCTACTGAAT10.7246376811594203No Hit
TTCATCAGCTGATGTGGTCATTATGTTCCATGGATGTTTTGCTGTCACTTC10.7246376811594203No Hit
GCTGTAAAGAAGTATATGAGGTTAATTGCTGAAAATCCAGTTTTGATGATC10.7246376811594203No Hit
GAGCAGATGCATCTGTGATCCATCCTCTTGCATCTGTCTCTTATACACATC10.7246376811594203No Hit
GGAACAGGGAGTTTCGGTAAGAGGAGGAACAAGAGTCACACACTCTGTGTG10.7246376811594203No Hit
GTTCTTAAGAAGAATCCATTGAAGAATCTCAATGTGATGTTGAAGTTGAAT10.7246376811594203No Hit
TCTACAGGATGCTGTTTTTTATACGCAGCAGGATTCGGCGTGTGCAAGAAC10.7246376811594203No Hit
CATTTTAATCCAAGATAACAGGTTGCGACTTTTTTATTTTATTTGTCAAAT10.7246376811594203No Hit
AGTGACTACTACCTCCCTTGTTTTCAGAGCAAGTTTCCTCATACGAATACG10.7246376811594203No Hit
TATCAGAATAAGGTACTTACCTACGGTATAGCTGGTGCAATTGCTGTCTCT10.7246376811594203No Hit
TGTGTAGAGCTCGTGGATGACATATATATTTTTCCGTTGTTAGCATCCATG10.7246376811594203No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers