FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_51.3410000000a908.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_51.3410000000a908.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707718
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC53890.761461486072136No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT7500.10597441353759549No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5050.039.65184445
TATACGG356.246639E-638.5698555
ATGCCGT5950.033.6540944
GCCCACG5900.033.55795318
CGAGCCC5900.033.55795315
TATGCCG6050.033.0978243
CGTATGC6250.032.03869241
AGCCCAC6200.031.93418117
CCGAGCC6200.031.93418114
GTATGCC6300.031.78441842
CCCACGA6400.031.63933219
GAGCCCA6400.030.93623716
CCACGAG6600.030.68056520
TCCGAGC6550.030.57127213
CTCCGAG6600.030.3396712
AGACTAG6800.030.10906425
TCGTATG6750.029.99877540
TATCTCG6850.029.8892936
CGAGACT6850.029.8892923
ACGAGAC6850.029.56083722
Produced by FastQC (version 0.11.2)