FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_50.3410000000a8fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_50.3410000000a8fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954279
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC48520.508446691166839TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT12050.12627334353999198No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5300.040.7526345
ATGCCGT6700.032.90876444
TATGCCG7100.030.73786443
CGAGACT7250.030.72256523
CCCACGA7300.030.51213519
GCCCACG7350.030.3045718
CCACGAG7400.030.0998120
CGTATGC7300.029.58752441
GTATGCC7300.029.58752442
AGCCCAC7650.029.11615617
CTCGTAT7650.028.5279539
TCGTATG7700.028.34270340
CACGAGA8050.027.66939221
CGAGCCC8150.027.32988715
ACGAGAC8250.026.99861522
CCGAGCC8300.026.83597614
TCTCGTA8250.026.4531938
TCCGAGC8450.026.35959613
AATCTCG8200.026.34011536
CAGCGTC608.192901E-626.24865540
Produced by FastQC (version 0.11.2)