FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_49.3410000000a8d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_49.3410000000a8d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574028
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC9010.15696098448159324RNA PCR Primer, Index 27 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1500.029.99912843
ACGGGCG300.005145868329.9991263
CAGGCAC300.005145868329.9991264
GCCCACG1600.029.53039418
CGTATGC1750.026.99921641
CCCACGA1750.026.99921619
CGAATTG1950.025.38387928
GTATGCC2000.024.7492842
TATCTCG1850.024.32361836
CGAGACG2000.023.62431323
ATGCCGT2000.023.62431344
ACGAATT2100.023.57074527
TCGTATG1950.023.07625440
GAATTGC2050.023.04811129
TAGACTG702.7005543E-522.4993465
ACCGCCT500.00226362122.4993468
TCCGAGC2250.021.99936113
GGTATCA2450.021.149471
TGCCGTC2250.020.9993945
GTATCAA5400.020.8599851
Produced by FastQC (version 0.11.2)