FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_39.3410000000a830.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_39.3410000000a830.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341630
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC20590.15347003272139115No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCGC301.1402407E-437.49878710
GAGTGCG250.00210756835.9988379
CTCGCGG250.00210756835.99883739
TGCCGTC2350.034.46697245
GCCCACG2500.031.49898518
CCCACGA2600.030.28748119
TATGCCG2750.029.45359443
CGAGCCC2800.028.92763715
AGCCCAC2850.027.63068817
ATGCCGT3000.026.99912844
CCGAGCC3150.025.71345314
CCACGAG3150.024.99919120
TCCGAGC3300.024.54466213
CGTATGC3400.023.8227641
TATCTCG3550.022.81616436
GTATGCC3700.022.49927342
TCGTATG3750.021.59930240
CTCCGAG3900.020.7685612
GAGCCCA3950.020.50566516
GCGGGAT550.00393761220.45388612
Produced by FastQC (version 0.11.2)