FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_38.3410000000a823.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_38.3410000000a823.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732290
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC14260.19473159540619153TruSeq Adapter, Index 12 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG2900.033.36080618
TATGCCG2900.033.36080643
CGTATGC3100.031.20849441
CCGAGCC3200.030.23322914
CTCGTAT3300.029.99886339
CCCACGA3400.029.1165419
ATGCCGT3350.028.87950144
CGAGACT3300.027.27169423
GTATGCC3550.027.25248742
TCGTATG3600.026.8739840
AGACTAC3500.026.35614425
CGAGCCC3700.026.14765715
AGCCCAC3800.026.05164317
TGCCGTC3750.025.7990245
CTCCGAG3850.025.12891612
ACGAGAC3850.025.12891622
GCGCGCG450.001226400724.9990529
CCGTGCG450.001226400724.9990529
CCGAGCG450.001226400724.9990529
TCCGAGC3950.024.49274313
Produced by FastQC (version 0.11.2)