FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_37.3410000000a809.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_37.3410000000a809.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202155
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC10720.5302861665553659RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.13108753184437683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1050.038.5703743
GCCCACG1100.036.8171718
CGTATGC1100.036.8171741
AGCCCAC1100.036.8171717
TATCTCG1100.036.8171736
ACGAATT1100.036.8171727
TCGCTCG250.002103295935.9990110
GTATGCC1150.035.21642342
ATGCCGT1150.035.21642344
CCCACGA1200.033.7490719
GAGCCCA1200.033.7490716
CGAGCCC1200.033.7490715
GACGAAT1200.033.7490726
CGAATTG1250.032.3991128
GCTATCT1300.031.1529934
CGAGACG1300.031.1529923
ACGAGAC1300.031.1529922
GTTCTGC300.005107028430.036321
CCGAGCC1350.029.99917614
CACGAGA1400.028.92777421
Produced by FastQC (version 0.11.2)