FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_3.3410000000a5c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_3.3410000000a5c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506284
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC15220.30062178540107926No Hit
GTACTTAAGAAGATTAAAAAACCAAAAGCAAGAAGAGTCATAGACTCAAAA7750.1530761390839924No Hit
ACATAATAGAGAGATAAAAGACATCAAACATAACCAACATGAAATCAACTA6160.12167084087192168No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6030.11910311208728698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2050.034.02274740
GCCCACG2150.033.48675518
CGTATGC2250.031.99845541
CCCACGA2250.031.99845519
GTATGCC2250.031.99845542
CGAGACT2300.031.30283523
TCCGAGC2350.030.63681813
CTCGTAT2400.029.9985539
CGAGCCC2400.029.9985515
TATGCCG2450.029.38633743
CCGAGCC2550.029.11624114
ATCCTAT2500.028.7986132
AGACTAG2550.028.2339325
ACGAGAC2550.028.2339322
GACTAGA2550.028.2339326
ATGCCGT2700.027.4986744
TATCTCG2550.027.35162236
TGCCGTC2650.027.16849945
GATCCTA2750.026.99869731
TCTCGTA2600.026.82562638
Produced by FastQC (version 0.11.2)